Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,229,626	T→C	T257A (ACT→GCT)	hlyE ←	hemolysin E

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,229,626	0	T	C	100.0%	42.1 / NA	13	T257A (ACT→GCT)	hlyE	hemolysin E
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (5/8);  total (5/8)

TTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTATTTCGGTGGTTAATTTCAATTTGGCGGCATCGATATCTTTATT  >  NC_000913/1229567‑1229698                                                            |                                                                         ttcttTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGCTTCCGTTTTTATCTCACCGAt                                                     >  2:221243/1‑81 (MQ=255)      ttAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGCTTCCGTTTTTATCTCACCGATggcgg                                                >  2:221227/1‑81 (MQ=255)      ttAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGCTTCCGTTTTTATCTCACCGATggcg                                                 <  1:221212/80‑1 (MQ=255)         gCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGCTTCCGTTTTTATCTCACCGATGGCGGCTa                                             >  2:221233/1‑81 (MQ=255)                     ttAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGCTTCCGTTTTTATCTCACCGATGGCGGCTATTTCGGTGGtt                                  >  2:221230/1‑80 (MQ=255)                                  aTCAACGTAGAATCTGGTTGTTTCAGCTTCCGTTTTTATCTCACCGATGGCGGCTATTTCGGTGGTTAATTTCAATTTggc                    <  1:221213/81‑1 (MQ=255)                                       cGTAGAATCTGGTTGTTTCAGCTTCCGTTTTTATCTCACCGATGGCGGCTATTTCGGTGGTTAATTTCAATTTGGCGGCAt               <  1:221214/81‑1 (MQ=255)                                        gTAGAATCTGGTTGTTTCAGCTTCCGTTTTTATCTCACCGATGGCGGCTATTTCGGTGGTTAATTTCAATTTGGCGGCATc              >  1:221231/1‑81 (MQ=255)                                        gTAGAATCTGGTTGTTTCAGCTTCCGTTTTTATCTCACCGATGGCGGCTATTTCGGTGGTTAATTTCAATTTGGCGGCATc              <  2:221215/81‑1 (MQ=255)                                         tAGAATCTGGTTGTTTCAGCTTCCGTTTTTATCTCACCGATGGCGGCTATTTCGGTGGTTAATTTCAATTTGGCGGCATCg             <  2:221216/81‑1 (MQ=255)                                                  gttgttTCAGCTTCCGTTTTTATCTCACCGATGGCGGCTATTTCGGTGGTTAATTTCAATTTGGCGGCATCGATATCtt      <  1:221217/79‑1 (MQ=255)                                                     gttTCAGCTTCCGTTTTTATCTCACCGATGGCGGCTATTTCGGTGGTTAATTTCAATTTGGCGGCATCGATATCTTTAtt  <  1:221218/80‑1 (MQ=255)                                                     gttTCAGCTTCCGTTTTTATCTCACCGATGGCGGCTATTTCGGTGGTTAATTTCAATTTGGCGGCATCGATATCTTTAtt  <  1:221219/80‑1 (MQ=255)                                                            |                                                                         TTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTATTTCGGTGGTTAATTTCAATTTGGCGGCATCGATATCTTTATT  >  NC_000913/1229567‑1229698

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑