Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	88,646	G→A	D207N (GAT→AAT)	cra →	DNA‑binding transcriptional dual regulator Cra

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	88,646	0	G	A	100.0%	125.8 / NA	36	D207N (GAT→AAT)	cra	DNA‑binding transcriptional dual regulator Cra
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (13/23);  total (13/23)

ACGGTGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCGGGAGGCGGCTGCCCAGTTATTCGAAAAAT  >  NC_000913/88568‑88721                                                                               |                                                                            aCGGTGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAaat                                                                           <  1:17909/81‑1 (MQ=255)    gTGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAaatgat                                                                        <  1:17910/81‑1 (MQ=255)       cTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAAATGATcc                                                                      <  1:17912/80‑1 (MQ=255)       cTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAAATGATcc                                                                      <  2:17911/80‑1 (MQ=255)                ggTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAAATGATCCGCGCGAAGTg                                                            >  2:349776/1‑81 (MQ=255)                 gTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAAATGATCCGCGCGAAGTGc                                                           <  2:17913/81‑1 (MQ=255)                   gcgcTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAAATGATCCGCGCGAAGTGCAt                                                         >  1:17960/1‑81 (MQ=255)                    cgcTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAAATGATCCGCGCGAAGTGCAt                                                         <  1:17914/80‑1 (MQ=255)                      cTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAAATGATCCGCGCGAAGTGCATTTc                                                      <  2:17915/81‑1 (MQ=255)                         ccGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAAATGATCCGCGCGAAGTGCATTTCCt                                                    <  1:17917/80‑1 (MQ=255)                         ccGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAAATGATCCGCGCGAAGTGCATTTCCTg                                                   <  1:17916/81‑1 (MQ=255)                          cGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAAATGATCCGCGCGAAGTGCATTTCCTGt                                                  <  1:17918/81‑1 (MQ=255)                          cGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAAATGATCCGCGCGAAGTGCATTTCCTGt                                                  <  1:17919/81‑1 (MQ=255)                          cGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAAATGATCCGCGCGAAGTGCATTTCCTGt                                                  <  1:17920/81‑1 (MQ=255)                           ggAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAAATGATCCGCGCGAAGTGCATTTCCTGTa                                                 >  1:17973/1‑81 (MQ=255)                            gAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAAATGATCCGCGCGAAGTGCATTTCCTGTAt                                                <  2:17921/81‑1 (MQ=255)                            gAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAAATGATCCGCGCGAAGTGCATTTCCTGTAt                                                >  2:17951/1‑81 (MQ=255)                             aGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAAATGATCCGCGCGAAGTGCATTTCCTGTATg                                               <  2:17922/81‑1 (MQ=255)                                tttCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAAATGATCCGCGCGAAGTGCATTTCCTGTATGcc                                             >  1:17962/1‑80 (MQ=255)                                 ttCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAAATGATCCGCGCGAAGTGCATTTCCTGTATGCCaa                                           >  1:17956/1‑81 (MQ=255)                                      tCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAAATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAg                                        <  2:17923/79‑1 (MQ=255)                                             ccTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAAATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGc                                <  1:17924/80‑1 (MQ=255)                                              cTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAAATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCgg                              <  2:17925/81‑1 (MQ=255)                                                 cGTGAACAAGGTTTCCGTACTGCCTGGAAAAATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCGGGAg                           >  2:17978/1‑81 (MQ=255)                                                     aaCAAGGTTTCCGTACTGCCTGGAAAAATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCGGGAggcgg                       >  2:17968/1‑81 (MQ=255)                                                     aaCAAGGTTTCCGTACTGCCTGGAAAAATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCGGGAggcgg                       >  2:17958/1‑81 (MQ=255)                                                       cAAGGTTTCCGTACTGCCTGGAAAAATGATCCGCACGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCGGGAGGCGGCt                     <  2:17926/81‑1 (MQ=255)                                                         aGGTTTCCGTACTGCCTGGAAAAATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCGGGAGGCGGCTGc                   <  2:17927/81‑1 (MQ=255)                                                          ggTTTCCGTACTGCCTGGAAAAATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCGGGAGGCGGCTGcc                  <  2:17928/81‑1 (MQ=255)                                                            tttCCGTACTGCCTGGAAAAATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCGGGAGGCGGCTGCCCa                <  1:17929/81‑1 (MQ=255)                                                            tttCCGTACTGCCTGGAAAAATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCGGGAGGCGGCTGCCCa                <  2:17930/81‑1 (MQ=255)                                                                cGTACTGCCTGGAAAAATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCGGGAGGCGGCTGCCCAGTTa            >  1:17963/1‑81 (MQ=255)                                                                 gTACTGCCTGGAAAAATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCGGGAGGCGGCTGCCCAGTTAt           <  1:17932/81‑1 (MQ=255)                                                                 gTACTGCCTGGAAAAATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCGGGAGGCGGCTGCCCAGTTAt           <  1:17931/81‑1 (MQ=255)                                                                  tACTGCCTGGAAAAATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCGGGAGGCGGCTGCCCAGTTAtt          <  1:17933/81‑1 (MQ=255)                                                                   aCTGCCTGGAAAAATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCGGGAGGCGGCTGCCCAGTTATTc         >  1:17971/1‑81 (MQ=255)                                                                      gCCTGGAAAAATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCGGGAGGCGGCTGCCCAGTTATTCGa       >  2:17983/1‑80 (MQ=255)                                                                          ggAAAAATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCGGGAGGCGGCTGCCCAGTTATTCGAAAAAt  >  1:17970/1‑81 (MQ=255)                                                                               |                                                                            ACGGTGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCGGGAGGCGGCTGCCCAGTTATTCGAAAAAT  >  NC_000913/88568‑88721

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑