Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,261,459	G→A	F139F (TTC→TTT)	prs ←	ribose‑phosphate diphosphokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,261,459	0	G	A	100.0%	96.5 / NA	28	F139F (TTC→TTT)	prs	ribose‑phosphate diphosphokinase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (12/16);  total (12/16)

GGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTG  >  NC_000913/1261385‑1261535                                                                           |                                                                             gggTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCAAAGaaa                                                                        >  1:226362/1‑81 (MQ=255)        ccAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCAAAGAAACCCTGaa                                                                 >  2:226351/1‑81 (MQ=255)        ccAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCAAAGAAACCCTGaa                                                                 >  1:226352/1‑81 (MQ=255)           gATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCAAAGAAACCCTGAATCt                                                              >  2:226359/1‑81 (MQ=255)                aGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCAAAGAAACCCTGAATCTGTTc                                                          <  2:226316/80‑1 (MQ=255)                 gCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCAAAGAAACCCTGAATCTGTTCAg                                                        <  1:226317/81‑1 (MQ=255)                  cTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCAAAGAAACCCTGAATCTGTTCAGc                                                       >  2:226365/1‑81 (MQ=255)                     cagcaTGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCAAAGAAACCCTGAATCTGTTCAGCGTg                                                    <  2:226318/81‑1 (MQ=255)                          tGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCAAAGAAACCCTGAATCTGTTCAGCGTGCAGAt                                               <  2:226319/81‑1 (MQ=255)                            tCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCAAAGAAACCCTGAATCTGTTCAGCGTGCAGATc                                              <  2:226320/80‑1 (MQ=255)                                 cagcagGATCGGGCTACCAAATACGTTATCAACCGGAACGTCAAAGAAACCCTGAATCTGTTCAGCGTGCAGATCCACTGt                                        >  1:226366/1‑81 (MQ=255)                                  agcagGATCGGGCTACCAAATACGTTATCAACCGGAACGTCAAAGAAACCCTGAATCTGTTCAGCGTGCAGATCCACTGTc                                       <  1:226321/81‑1 (MQ=255)                                      ggATCGGGCTACCAAATACGTTATCAACCGGAACGTCAAAGAAACCCTGAATCTGTTCAGCGTGCAGATCCACTGTCAGca                                   >  1:226370/1‑81 (MQ=255)                                       gATCGGGCTACCAAATACGTTATCAACCGGAACGTCAAAGAAACCCTGAATCTGTTCAGCGTGCAGATCCACTGTCAGcac                                  <  2:226322/81‑1 (MQ=255)                                         tCGGGCTACCAAATACGTTATCAACCGGAACGTCAAAGAAACCCTGAATCTGTTCAGCGTGCAGATCCACTGTCAGCacac                                <  1:226323/81‑1 (MQ=255)                                          cGGGCTACCAAATACGTTATCAACCGGAACGTCAAAGAAACCCTGAATCTGTTCAGCGTGCAGATCCACTGTCAGCACACg                               >  1:226369/1‑81 (MQ=255)                                          cGGGCTACCAAATACGTTATCAACCGGAACGTCAAAGAAACCCTGAATCTGTTCAGCGTGCAGATCCACTGTCAGCACACg                               >  2:226364/1‑81 (MQ=255)                                           gggCTACCAAATACGTTATCAACCGGAACGTCAAAGAAACCCTGAATCTGTTCAGCGTGCAGATCCACTGTCAGCACACgg                              >  1:226368/1‑81 (MQ=255)                                              cTACCAAATACGTTATCAACCGGAACGTCAAAGAAACCCTGAATCTGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCa                           <  1:226324/81‑1 (MQ=255)                                                 ccAAATACGTTATCAACCGGAACGTCAAAGAAACCCTGAATCTGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAac                         <  2:226325/80‑1 (MQ=255)                                                        cGTTATCAACCGGAACGTCAAAGAAACCCTGAATCTGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGc                 >  2:226361/1‑81 (MQ=255)                                                            aTCAACCGGAACGTCAAAGAAACCCTGAATCTGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTgg              <  1:226327/80‑1 (MQ=255)                                                            aTCAACCGGAACGTCAAAGAAACCCTGAATCTGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTgg              <  2:226326/80‑1 (MQ=255)                                                                aCCGGAACGTCAAAGAAACCCTGAATCTGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAgg         <  2:226328/81‑1 (MQ=255)                                                                  cGGAACGTCAAAGAAACCCTGAATCTGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAgg         >  1:226377/1‑79 (MQ=255)                                                                    gAACGTCAAAGAAACCCTGAATCTGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGt     <  1:226329/81‑1 (MQ=255)                                                                     aaCGTCAAAGAAACCCTGAATCTGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTc    <  1:226330/81‑1 (MQ=255)                                                                       cGTCAAAGAAACCCTGAATCTGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTg  <  2:226331/81‑1 (MQ=255)                                                                           |                                                                             GGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTG  >  NC_000913/1261385‑1261535

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑