Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,280,267	C→T	A135V (GCA→GTA)	narG →	nitrate reductase A subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,280,267	0	C	T	100.0%	105.7 / NA	31	A135V (GCA→GTA)	narG	nitrate reductase A subunit alpha
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (19/12);  total (19/12)

AATACCCGATGATGCGCAAACGCCTGATGAAAATGTGGCGTGAAGCGAAGGCGCTGCATAGCGATCCGGTTGAGGCATGGGCTTCTATCATTGAAGACGCCGATAAAGCGAAAAGCTTTAAGCAGGCGCGTGGACGCGGTGGATTTGTTCGTT  >  NC_000913/1280192‑1280344                                                                            |                                                                              aaTACCCGATGATGCGCAAACGCCTGATGAAAATGTGGCGTGAAGCGAAGGCGCTGCATAGCGATCCGGTTGAGGTATggg                                                                          <  1:229356/81‑1 (MQ=255)       cGATGATGCGCAAACGCCTGATGAAAATGTGGCGTGAAGCGAAGGCGCTGCATAGCGATCCGGTTGAGGTATGGGCTTCTa                                                                    >  2:229382/1‑81 (MQ=255)        gatgatGCGCAAACGCCTGATGAAAATGTGGCGTGAAGCGAAGGCGCTGCATAGCGATCCGGTTGAGGTATGGGCTTCTAt                                                                   >  1:229376/1‑81 (MQ=255)            atgCGCAAACGCCTGATGAAAATGTGGCGTGAAGCGAAGGCGCTGCATAGCGATCCGGTTGAGGTATGGGCTTCTATCAt                                                                <  1:229357/80‑1 (MQ=255)               cgcAAACGCCTGATGAAAATGTGGCGTGAAGCGAAGGCGCTGCATAGCGATCCGGTTGAGGTATGGGCTTCTATCATTg                                                              >  2:229379/1‑79 (MQ=255)               cgcAAACGCCTGATGAAAATGTGGCGTGAAGCGAAGGCGCTGCATAGCGATCCGGTTGAGGTATGGGCTTCTATCATTGaa                                                            <  1:229358/81‑1 (MQ=255)                gcAAACGCCTGATGAAAATGTGGCGTGAAGCGAAGGCGCTGCATAGCGATCCGGTTGAGGTATGGGCTTCTATCATTg                                                              >  1:229378/1‑78 (MQ=255)                  aaaCGCCTGATGAAAATGTGGCGTGAAGCGAAGGCGCTGCATAGCGATCCGGTTGAGGTATGGGCTTCTATCATTGAAGAc                                                         >  2:229377/1‑81 (MQ=255)                    aCGCCTGATGAAAATGTGGCGTGAAGCGAAGGCGCTGCATAGCGATCCGGTTGAGGTATGGGCTTCTATCATTGAAGACGc                                                       >  1:229386/1‑81 (MQ=255)                      gCCTGATGAAAATGTGGCGTGAAGCGAAGGCGCTGCATAGCGATCCGGTTGAGGTATGGGCTTCTATCATTGAAGACGCCg                                                     <  1:229359/81‑1 (MQ=255)                        cTGATGAAAATGTGGCGTGAAGCGAAGGCGCTGCATAGCGATCCGGTTGAGGTATGGGCTTCTATCATTGAAGACGCCGa                                                    >  2:229388/1‑80 (MQ=255)                             gaAAATGTGGCGTGAAGCGAAGGCGCTGCATAGCGATCCGGTTGAGGTATGGGCTTCTATCATTGAAGACGCCGATAAAGc                                              >  1:229392/1‑81 (MQ=255)                               aaaTGTGGCGTGAAGCGAAGGCGCTGCATAGCGATCCGGTTGAGGTATGGGCTTCTATCATTGAAGACGCCGATAAAGCGa                                            <  1:229360/81‑1 (MQ=255)                                        gTGAAGCGAAGGCGCTGCATAGCGATCCGGTTGAGGTATGGGCTTCTATCATTGAAGACGCCGATAAAGCGAAAAGCTTTa                                   <  1:229361/81‑1 (MQ=255)                                           aaGCGAAGGCGCTGCATAGCGATCCGGTTGAGGTATGGGCTTCTATCATTGAAGACGCCGATAAAGCGAAAAGCTTTAAGc                                <  1:229362/81‑1 (MQ=255)                                            aGCGAAGGCGCTGCATAGCGATCCGGTTGAGGTATGGGCTTCTATCATTGAAGACGCCGATAAAGCGAAAAGCTTTAAg                                 >  2:229385/1‑79 (MQ=255)                                              cGAAGGCGCTGCATAGCGATCCGGTTGAGGTATGGGCTTCTATCATTGAAGACGCCGATAAAGCGAAAAGCTTTAAGCAgg                             <  2:229363/81‑1 (MQ=255)                                                 aGGCGCTGCATAGCGATCCGGTTGAGGTATGGGCTTCTATCATTGAAGACGCCGATAAAGCGAAAAGCTTTAAGCAGgcgc                          <  2:229364/81‑1 (MQ=255)                                                  ggCGCTGCATAGCGATCCGGTTGAGGTATGGGCTTCTATCATTGAAGACGCCGATAAAGCGAAAAGCTTTAAGCAGGcgcg                         >  1:229397/1‑81 (MQ=255)                                                    cgcTGCATAGCGTTCCGGTTGAGGTATGGGCTTCTATCATTGAAGACGCCGATAAAGCGAAAAGCTTTAAGCAGGCGCGTg                       >  2:229400/1‑81 (MQ=255)                                                    cgcTGCATAGCGATCCGGTTGAGGTATGGGCTTCTATCATTGAAGACGCCGATAAAGCGAAAAGCTTTAAGCAGGCGCGTg                       <  2:229365/81‑1 (MQ=255)                                                    cgcTGCATAGCGATCCGGTTGAGGTATGGGCTTCTATCATTGAAGACGCCGATAAAGCGAAAAGCTTTAAGCAGGCGCGTg                       >  1:229398/1‑81 (MQ=255)                                                     gcTGCATAGCGATCCGGTTGAGGTATGGGCTTCTATCATTGAAGACGCCGATAAAGCGAAAAGCTTTAAGCAGGCGCGTgg                      <  2:229366/81‑1 (MQ=255)                                                     gcTGCATAGCGATCCGGTTGAGGTATGGGCTTCTATCATTGAAGACGCCGATAAAGCGAAAAGCTTTAAGCAGGCGCGTg                       >  2:229393/1‑80 (MQ=255)                                                          aTAGCGATCCGGTTGAGGTATGGGCTTCTATCATTGAAGACGCCGATAAAGCGAAAAGCTTTAAGCAGGCGCGTGGAcgcg                 >  2:229390/1‑81 (MQ=255)                                                           tAGCGATCCGGTTGAGGTATGGGCTTCTATCATTGAAGACGCCGATAAAGCGAAAAGCTTTAAGCAGGCGCGTGGACGCgg                >  1:229413/1‑81 (MQ=255)                                                                  ccGGTTGAGGTATGGGCTTCTATCATTGAAGACGCCGATAAAGCGAAAAGCTTTAAGCAGGCGCGTGGACGCGGTGGAttt         >  2:229383/1‑81 (MQ=255)                                                                  ccGGTTGAGGTATGGGCTTCTATCATTGAAGACGCCGATAAAGCGAAAAGCTTTAAGCAGGCGCGTGGACGCGGTGGAttt         >  2:229399/1‑81 (MQ=255)                                                                  ccGGTTGAGGTATGGGCTTCTATCATTGAAGACGCCGATAAAGCGAAAAGCTTTAAGCAGGCGCGTGGACGCGGTGGAttt         >  1:229384/1‑81 (MQ=255)                                                                         aGGTATGGGCTTCTATCATTGAAGACGCCGATAAAGCGAAAAGCTTTAAGCAGGCGCGTGGACGCGGTGGATTTgttcgtt  >  1:229391/1‑81 (MQ=255)                                                                         aGGTATGGGCTTCTATCATTGAAGACGCCGATAAAGCGAAAAGCTTTAAGCAGGCGCGTGGACGCGGTGGATTTgttcgt   <  2:229367/80‑1 (MQ=255)                                                                            |                                                                              AATACCCGATGATGCGCAAACGCCTGATGAAAATGTGGCGTGAAGCGAAGGCGCTGCATAGCGATCCGGTTGAGGCATGGGCTTCTATCATTGAAGACGCCGATAAAGCGAAAAGCTTTAAGCAGGCGCGTGGACGCGGTGGATTTGTTCGTT  >  NC_000913/1280192‑1280344

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑