Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,290,291	A→C	Q17P (CAG→CCG)	rssB →	regulator of RpoS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,290,291	0	A	C	100.0%	74.6 / NA	22	Q17P (CAG→CCG)	rssB	regulator of RpoS
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (17/5);  total (17/5)

AGTAAAGCCAGTCAGGGGAGAGAACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGTTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGAT  >  NC_000913/1290217‑1290367                                                                           |                                                                             aGTAAAGCCAGTCAGGGGAGAGAACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCCGGTAtt                                                                        >  2:231210/1‑81 (MQ=255)  gTAAAGCCAGTCAGGGGAGAGAACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCCGGTAttt                                                                       <  2:231192/81‑1 (MQ=255)    aaaGCCAGTCAGGGGAGAGAACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCCGGTATTtcg                                                                     >  2:231213/1‑81 (MQ=255)         cAGTCAGGGGAGAGAACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCCGGTATTtcgctcgc                                                                >  1:231214/1‑81 (MQ=255)          aGTCAGGGGAGAGAACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCCGGTATTTcgctcgct                                                               <  2:231193/81‑1 (MQ=255)              aGGGGAGAGAACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCCGGTATTTCGCTCGCTTCt                                                            <  1:231194/80‑1 (MQ=255)                          aTGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCCGGTATTTCGCTCGCTTCTGGATTCATGGttt                                               >  1:231208/1‑81 (MQ=255)                            gACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCCGGTATTTCGCTCGCTTCTGGATTCATGGTTTTc                                             >  2:231227/1‑81 (MQ=255)                                  gCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCCGGTATTTCGCTCGCTTCTGGATTCATGGTTTTCCTCAtt                                       <  1:231195/81‑1 (MQ=255)                                       tGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCCGGTATTTCGCTCGCTTCTGGATTCATGGTTTTCCTCATTGGGAg                                  >  2:231224/1‑81 (MQ=255)                                        ggTCGGAAAACAGATTCTCATTGTTGAAGATGAGCCGGTATTTCGCTCGCTTCTGGATTCATGGTTTTCCTCATTGGGAGc                                 >  1:231218/1‑81 (MQ=255)                                         gTCGGAAAACAGATTCTCATTGTTGAAGATGAGCCGGTATTTCGCTCGCTTCTGGATTCATGGTTTTCCTCATTGGGAGc                                 >  2:231220/1‑80 (MQ=255)                                           cGGAAAACAGATTCTCATTGTTGAAGATGAGCCGGTATTTCGCTCGCTTCTGGATTCATGGTTTTCCTCATTGGGAGCGa                               >  1:231226/1‑80 (MQ=255)                                            ggAAAACAGATTCTCATTGTTGAAGATGAGCCGGTATTTCGCTCGCTTCTGGATTCATGGTTTTCCTCATTGGGAGCGAc                              >  1:231222/1‑80 (MQ=255)                                                 aCAGATTCTCATTGTTGAAGATGAGCCGGTATTTCGCTCGCTTCTGGATTCATGGTTTTCCTCATTGGGAGCGACAACgg                         >  1:231223/1‑80 (MQ=255)                                                              gttgAAGATGAGCCGGTATTTCGCTCGCTTCTGGATTCATGGTTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGAt           >  2:231225/1‑81 (MQ=255)                                                                tgAAGATGAGCCGGTATTTCGCTCGCTTCTGGATTCATGGTTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATgg         >  1:231228/1‑81 (MQ=255)                                                                 gAAGATGAGCCGGTATTTCGCTCGCTTCTGGATTCATGGTTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATggg        >  1:231233/1‑81 (MQ=255)                                                                   aGATGAGCCGGTATTTCGCTCGCTTCTGGATTCATGGTTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGt      >  2:231221/1‑81 (MQ=255)                                                                   aGATGAGCCGGTATTTCGCTCGCTTCTGGATTCATGGTTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGt      <  1:231196/81‑1 (MQ=255)                                                                    gATGAGCCGGTATTTCGCTCGCTTCTGGATTCATGGTTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTg     >  1:231232/1‑81 (MQ=255)                                                                       gAGCCGGTATTTCGCTCGCTTCTGGATTCATGGTTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGAt  >  1:231235/1‑81 (MQ=255)                                                                           |                                                                             AGTAAAGCCAGTCAGGGGAGAGAACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGTTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGAT  >  NC_000913/1290217‑1290367

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑