Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,294,564	G→T	pseudogene (759/897 nt)	insZ ←	putative transposase InsZ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,294,564	0	G	T	100.0%	62.1 / NA	19	pseudogene (759/897 nt)	insZ	putative transposase InsZ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (7/12);  total (7/12)

GGCCTTGATGGTCGGGGGCGTTTTGTTATGAACACCACTTCCAGAGCACCCCGAAGATGCTCCAGTCGTTTCGGGATGGTCCCTGGTGACGCCGTGTTTCCCAGCTCTATCATTTCTGATGCGATATTCTTCCACGCAGGCAGTAGC  >  NC_000913/1294490‑1294636                                                                           |                                                                         ggCCTTGATGGTCGGGGGCGTTTTGTTATGAACACCACTTCCAGAGCACCCCGAAGATGCTCCAGTCGTTTCGGTATGGTc                                                                    <  2:231715/81‑1 (MQ=255)        aTGGTCGGGGGCGTTTTGTTATGAACACCACTTCCAGAGCACCCCGAAGATGCTCCAGTCGTTTCGGTATGGTCCCTGGTg                                                             <  1:231716/81‑1 (MQ=255)              gggggCGTTTTGTTATGAACACCACTTCCAGAGCACCCCGAAGATGCTCCAGTCGTTTCGGTATGGTCCCTGGTGACGcc                                                        <  2:231717/80‑1 (MQ=255)                gggCGTTTTGTTATGAACACCACTTCCAGAGCACCCCGAAGATGCTCCAGTCGTTTCGGTATGGTCCCTGGTGACGCCgtg                                                     >  1:231738/1‑81 (MQ=255)                    gTTTTGTTATGAACACCACTTCCAGAGCACCCCGAAGATGCTCCAGTCGTTTCGGTATGGTCCCTGGTGACGCCGTGTTTc                                                 <  2:231718/81‑1 (MQ=255)                     ttttGTTATGAACACCACTTCCAGAGCACCCCGAAGATGCTCCAGTCGTTTCGGTATGGTCCCTGGTGACGCCGTGTTTcc                                                >  2:231741/1‑81 (MQ=255)                        tGTTATGAACACCACTTCCAGAGCACCCCGAAGATGCTCCAGTCGTTTCGGTATGGTCCCTGGTGACGCCGTGTTTCCCa                                              <  1:231719/80‑1 (MQ=255)                          ttATGAACACCACTTCCAGAGCACCCCGAAGATGCTCCAGTCGTTTCGGTATGGTCCCTGGTGACGCCGTGTTTCCCAGct                                           <  1:231720/81‑1 (MQ=255)                               aaCACCACTTCCAGAGCACCCCGAAGATGCTCCAGTCGTTTCGGTATGGTCCCTGGTGACGCCGTGTTTCCCAGCTCTATc                                      <  2:231721/81‑1 (MQ=255)                                  accacTTCCAGAGCACCCCGAAGATGCTCCAGTCGTTTCGGTATGGTCCCTGGTGACGCCGTGTTTCCCAGCTCTATCAtt                                   <  1:231722/81‑1 (MQ=255)                                      cTTCCAGAGCACCCCGAAGATGCTCCAGTCGTTTCGGTATGGTCCCTGGTGACGCCGTGTTTCCCAGCTCTATCATTTCTg                               <  2:231723/81‑1 (MQ=255)                                             agCACCCCGAAGATGCTCCAGTCGTTTCGGTATGGTCCCTGGTGACGCCGTGTTTCCCAGCTCTATCATTTCTGATGCGat                        <  2:231724/81‑1 (MQ=255)                                              gCACCCCGAAGATGCTCCAGTCGTTTCGGTATGGTCCCTGGTGACGCCGTGTTTCCCAGCTCTATCATTTCTGATGCGata                       >  2:231742/1‑81 (MQ=255)                                                     gAAGATGCTCCAGTCGTTTCGGTATGGTCCCTGGTGACGCCGTGTTTCCCAGCTCTATCATTTCTGATGCGATAttcttc                 >  2:231749/1‑80 (MQ=255)                                                              ccAGTCGTTTCGGTATGGTCCCTGGTGACGCCGTGTTTCCCAGCTCTATTATTTCTGATGCGATATTCTTCCACgcagg         >  2:231743/1‑79 (MQ=255)                                                                aGTCGTTTCGGTATGGTCCCTGGTGACGCCGTGTTTCCCAGCTCTATCATTTCTGATGCGATATTCTTCCACgcaggcag      >  1:231745/1‑80 (MQ=255)                                                                 gTCGTTTCGGTATGGTCCCTGGTGACGCCGTGTTTCCCAGCTCTATCATTTCTGATGCGATATTCTTCCACgcaggcag      >  1:231739/1‑79 (MQ=255)                                                                 gTCGTTTCGGTATGGTCCCTGGTGACGCCGTGTTTCCCAGCTCTATCATTTCTGATGCGATATTCTTCCACGCAGGCAGTa    <  2:231725/81‑1 (MQ=255)                                                                    gTTTCGGTATGGTCCCTGGTGACGCCGTGTTTCCCAGCTCTATCATTTCTGATGCGATATTCTTCCACGCAGGCAGTAGc  <  1:231726/80‑1 (MQ=255)                                                                           |                                                                         GGCCTTGATGGTCGGGGGCGTTTTGTTATGAACACCACTTCCAGAGCACCCCGAAGATGCTCCAGTCGTTTCGGGATGGTCCCTGGTGACGCCGTGTTTCCCAGCTCTATCATTTCTGATGCGATATTCTTCCACGCAGGCAGTAGC  >  NC_000913/1294490‑1294636

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑