Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,297,921	G→A	I67I (ATC→ATT)	adhE ←	aldehyde‑alcohol dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,297,921	0	G	A	100.0%	26.7 / NA	9	I67I (ATC→ATT)	adhE	aldehyde‑alcohol dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (6/3);  total (6/3)

AGACAGAACACCACAGGTTTTTTCATCTTTATAGGCGTTGTAGATATATTCAGAAGCAAAGTGGTTTTTGATCACTTTATCTTCGACGATACCCATGCCGGATTCGGCAACGGCCATTTTCGCGAGTGGGATTCGAG  >  NC_000913/1297852‑1297988                                                                      |                                                                    agacagaACACCACAGGTTTTTTCATCTTTATAGGCGTTGTAGATATATTCAGAAGCAAAGTGGTTTTTAATCACTTTAt                                                           <  2:232291/80‑1 (MQ=255)   acagaACACCACAGGTTTTTTCATCTTTATAGGCGTTGTAGATATATTCAGAAGCAAAGTGGTTTTTAATCACTTTATCtt                                                        >  2:232309/1‑81 (MQ=255)    cagaACACCACAGGTTTTTTCATCTTTATAGACGTTGTAGATATATTCAGAAGCAAAGTGGTTTTTAATCACTTTATCTTc                                                       <  2:232292/81‑1 (MQ=255)         caccacAGGTTTTTTCATCTTTATGGGCGTTGTAGATATATTCAGAAGCAAAGTGGTTTTTAATCACTTTATCTTcgacga                                                  <  1:232293/81‑1 (MQ=255)                                 aGGCGTTGTAGATATATTCAGAAGCAAAGTGGTTTTTAATCACTTTATCTTCGACGATACCCATGCCGGATTCGGCAACgg                          >  1:232310/1‑81 (MQ=255)                                         tAGATATATTCAGAAGCAAAGTGGTTTTTAATCACTTTATCTTCGACGATACCCATGCCGGATTCGGCAACGGCCATTTTc                  >  1:232312/1‑81 (MQ=255)                                                    aGAAGCAAAGTGGTTTTTAATCACTTTATCTTCGACGATACCCATGCCGGATTCGGCAACGGCCATTTTCGCGAGTGGGAt       >  2:232317/1‑81 (MQ=255)                                                          aaaGTGGTTTTTAATCACTTTATCTTCGACGATACCCATGCCGGATTCGGCAACGGCCATTTTCGCGAGTGGGATTCGAg  >  2:232314/1‑80 (MQ=255)                                                          aaaGTGGTTTTTAATCACTTTATCTTCGACGATACCCATGCCGGATTCGGCAACGGCCATTTTCGCGAGTGGGATTCGAg  >  2:232319/1‑80 (MQ=255)                                                                      |                                                                    AGACAGAACACCACAGGTTTTTTCATCTTTATAGGCGTTGTAGATATATTCAGAAGCAAAGTGGTTTTTGATCACTTTATCTTCGACGATACCCATGCCGGATTCGGCAACGGCCATTTTCGCGAGTGGGATTCGAG  >  NC_000913/1297852‑1297988

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑