Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,301,472	C→T	V97V (GTC→GTT)	oppA →	oligopeptide ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,301,472	0	C	T	100.0%	117.3 / NA	34	V97V (GTC→GTT)	oppA	oligopeptide ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (14/20);  total (14/20)

CTGGTCAGCGATCTTGACGGTCATCCAGCACCTGGCGTCGCTGAATCCTGGGATAATAAAGACGCGAAAGTCTGGACCTTCCATTTGCGTAAAGATGCGAAATGGTCTGATGGCACGCCAGTCACAGCACAAGACTTTGTGTATAGCTG  >  NC_000913/1301401‑1301549                                                                        |                                                                              cTGGTCAGCGATCTTGACGGTCATCCAGCACCTGGCGTCGCTGAATCCTGGGATAATAAAGACGCGAAAGTTTGGACCTTc                                                                      <  2:232757/81‑1 (MQ=255)  tGGTCAGCGATCTTGACGGTCATCCAGCACCTGGCGTCGCTGAATCCTGGGATAATAAAGACGCGAAAGTTTGGACCTTc                                                                      >  2:232791/1‑80 (MQ=255)    gTCAGCGATCTTGACGGTCATCCAGCACCTGGCGTCGCTGAATCCTGGGATAATAAAGACGCGAAAGTTTGGACCTTCCAt                                                                   <  1:232758/81‑1 (MQ=255)        gCGATCTTGACGGTCATCCAGCACCTGGCGTCGCTGAATCCTGGGATAATAAAGACGCGAAAGTTTGGACCTTCCATTTGc                                                               >  2:232800/1‑81 (MQ=255)          gATCTTGACGGTCATCCAGCACCTGGCGTCGCTGAATCCTGGGATAATAAAGACGCGAAAGTTTGGACCTTCCATTTGCg                                                              >  2:232793/1‑80 (MQ=255)                gACGGTCATCCAGCACCTGGCGTCGCTGAATCCTGGGATAATAAAGACGCGAAAGTTTGGACCTTCCATTTGCGTaaa                                                          <  2:232760/78‑1 (MQ=255)                gACGGTCATCCAGCACCTGGCGTCGCTGAATCCTGGGATAATAAAGACGCGAAAGTTTGGACCTTCCATTTGCGTAAAGa                                                        <  2:232759/80‑1 (MQ=255)                 aCGGTCATCCAGCACCTGGCGTCGCTGAATCCTGGGATAATAAAGACGCGAAAGTTTGGACCTTCCATTTGCGTAAAGATg                                                      >  2:232804/1‑81 (MQ=255)                  cGGTCATCCAGCACCTGGCGTCGCTGAATCCTGGGATAATAAAGACGCGAAAGTTTGGACCTTCCATTTGCGTAAAGATGc                                                     <  2:232761/81‑1 (MQ=255)                   ggTCATCCAGCACCTGGCGTCGCTGAATCCTGGGATAATAAAGACGCGAAAGTTTGGACCTTCCATTTGCGTAAAGATGCg                                                    <  2:232762/81‑1 (MQ=255)                      cATCCAGCACCTGGCGTCGCTGAATCCTGGGATAATAAAGACGCGAAAGTTTGGACCTTCCATTTGCGTAAAGATGCGaaa                                                 >  2:232802/1‑81 (MQ=255)                          cAGCACCTGGCGTCGCTGAATCCTGGGATAATAAAGACGCGAAAGTTTGGACCTTCCATTTGCGTAAAGATGCGAAATGGt                                             <  2:232763/81‑1 (MQ=255)                           aGCACCTGGCGTCGCTGAATCCTGGGATAATAAAGACGCGAAAGTTTGGACCTTCCATTTGCGTAAAGATGCGAAATGGTc                                            >  1:232816/1‑81 (MQ=255)                           aGCACCTGGCGTCGCTGAATCCTGGGATAATAAAGACGCGAAAGTTTGGACCTTCCATTTGCGTAAAGATGCGAAATGGTc                                            >  2:232810/1‑81 (MQ=255)                            gCACCTGGCGTCGCTGAATCCTGGGATAATAAAGACGCGAAAGTTTGGACCTTCCATTTGCGTAAAGATGCGAAATGGTCt                                           <  1:232765/81‑1 (MQ=255)                            gCACCTGGCGTCGCTGAATCCTGGGATAATAAAGACGCGAAAGTTTGGACCTTCCATTTGCGTAAAGATGCGAAATGGTCt                                           <  1:232764/81‑1 (MQ=255)                                        gCTGAATCCTGGGATAATAAAGACGCGAAAGTTTGGACCTTCCATTTGCGTAAAGATGCGAAATGGTCTGATGGCACGc                                 >  2:232801/1‑79 (MQ=255)                                        gCTGAATCCTGGGATAATAAAGACGCGAAAGTTTGGACCTTCCATTTGCGTAAAGATGCGAAATGGTCTGATGGCACGc                                 >  2:232803/1‑79 (MQ=255)                                            aaTCCTGGGATAATAAAGACGCGAAAGTTTGGACCTTCCATTTGCGTAAAGATGCGAAATGGTCTGATGGCACGCCAGTc                            <  2:232766/80‑1 (MQ=255)                                            aaTCCTGGGATAATAAAGACGCGAAAGTTTGGACCTTCCATTTGCGTAAAGATGCGAAATGGTCTGATGGCACGCCAGTc                            <  2:232767/80‑1 (MQ=255)                                             aTCCTGGGATAATAAAGACGCGAAAGTTTGGACCTTCCATTTGCGTAAAGATGCGAAATGGTCTGATGGCACGCCAGTcac                          <  1:232768/81‑1 (MQ=255)                                             aTCCTGGGATAATAAAGACGCGAAAGTTTGGACCTTCCATTTGCGTAAAGATGCGAAATGGTCTGATGGCACGCCAGTca                           >  1:232821/1‑80 (MQ=255)                                                   ggATAATAAAGACGCGAAAGTTTGGACCTTCCATTTGCGTAAAGATGCGAAATGGTCTGATGGCACGCCAGTCACAGcac                     >  2:232809/1‑80 (MQ=255)                                                   ggATAATAAAGACGCGAAAGTTTGGACCTTCCATTTGCGTAAAGATGCGAAATGGTCTGATGGCACGCCAGTCACAGca                      <  2:232769/79‑1 (MQ=255)                                                     ataataAAGACGCGAAAGTTTGGACCTTCCATTTGCGTAAAGATGCGAAATGGTCTGATGGCACGCCAGTCACAGCACAAg                  <  2:232770/81‑1 (MQ=255)                                                      taataaAGACGCGAAAGTTTGGACCTTCCATTTGCGTAAAGATGCGAAATGGTCTGATGGCACGCCAGTCACAGCACAAGa                 >  2:232812/1‑81 (MQ=255)                                                         taaAGACGCGAAAGTTTGGACCTTCCATTTGCGTAAAGATGCGAAATGGTCTGATGGCACGCCAGTCACAGCACAAGACtt              >  1:232806/1‑81 (MQ=255)                                                           aaGACGCGAAAGTTTGGACCTTCCATTTGCGTAAAGATGCGAAATGGTCTGATGGCACGCCAGTCACAGCACAAGACttt             <  1:232772/80‑1 (MQ=255)                                                           aaGACGCGAAAGTTTGGACCTTCCATTTGCGTAAAGATGCGAAATGGTCTGATGGCACGCCAGTCACAGCACAAGACttt             <  1:232771/80‑1 (MQ=255)                                                              aCGCGAAAGTTTGGACCTTCCATTTGCGTAAAGATGCGAAATGGTCTGATGGCACGCCAGTCACAGCACAAGACTTTgtgt         <  2:232773/81‑1 (MQ=255)                                                              aCGCGAAAGTTTGGACCTTCCATTTGCGTAAAGATGCGAAATGGTCTGATGGCACGCCAGTCACAGCACAAGACTTTgtgt         <  1:232774/81‑1 (MQ=255)                                                               cgcgAAAGTTTGGACCTTCCATTTGCGTAAAGATGCGAAATGGTCTGATGGCACGCCAGTCACAGCACAAGACTTTGTGta        <  2:232775/81‑1 (MQ=255)                                                                  gAAAGTTTGGACCTTCCATTTGCGTAAAGATGCGAAATGGTCTGATGGCACGCCAGTCACAGCACAAGACTTTGTGTATAg     >  1:232813/1‑81 (MQ=255)                                                                     aGTTTGGACCTTCCATTTGCGTAAAGATGCGAAATGGTCTGATGGCACGCCAGTCACAGCACAAGACTTTGTGTATAGCTg  <  2:232776/81‑1 (MQ=255)                                                                        |                                                                              CTGGTCAGCGATCTTGACGGTCATCCAGCACCTGGCGTCGCTGAATCCTGGGATAATAAAGACGCGAAAGTCTGGACCTTCCATTTGCGTAAAGATGCGAAATGGTCTGATGGCACGCCAGTCACAGCACAAGACTTTGTGTATAGCTG  >  NC_000913/1301401‑1301549

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑