Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,319,991	G→A	P465L (CCG→CTG)	trpD ←	anthranilate synthase subunit TrpD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,319,991	0	G	A	100.0%	100.5 / NA	29	P465L (CCG→CTG)	trpD	anthranilate synthase subunit TrpD
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (16/13);  total (16/13)

CGCAGCGACGGCTGCTTCATGGGCGGCGTCGCCTTTACCTTGTAACAAACGTGTTAAAATGTCACGGTTTTCTTCCGGTGTTCCGCCTGCCAGTTGCTCCTGGTGGTAGGGTGTCAGGCCAAAGTCTTCTGCGGTGAGCTGATAGCTTT  >  NC_000913/1319915‑1320063                                                                             |                                                                         cGCAGCGACGGCTGCTTCATGGGCGGCGTCGCCTTTACCTTGTAACAAACGTGTTAAAATGTCACGGTTTTCTTCCAgtgt                                                                      >  1:235468/1‑81 (MQ=255) cGCAGCGACGGCTGCTTCATGGGCGGCGTCGCCTTTACCTTGTAACAAACGTGTTAAAATGTCACGGTTTTCTTCCAgtgt                                                                      >  1:235458/1‑81 (MQ=255)  gCAGCGACGGCTGCTTCATGGGCGGCGTCGCCTTTACCTTGTAACAAACGTGTTAAAATGTCACGGTTTTCTTCCAgtgt                                                                      <  1:235428/80‑1 (MQ=255)  gCAGCGACGGCTGCTTCATGGGCGGCGTCGCCTTTACCTTGTAACAAACGTGTTAAAATGTCACGGTTTTCTTCCAGTGtt                                                                     >  2:235453/1‑81 (MQ=255)  gCAGCGACGGCTGCTTCATGGGCGGCGTCGCCTTTACCTTGTAACAAACGTGTTAAAATGTCACGGTTTTCTTCCAGTGtt                                                                     <  1:235427/81‑1 (MQ=255)       gACGGCTGCTTCATGGGCGGCGTCGCCTTTACCTTGTAACAAACGTGTTAAAATGTCACGGTTTTCTTCCAGTGTTCCGcc                                                                >  1:235466/1‑81 (MQ=255)       gACGGCTGCTTCATGGGCGGCGTCGCCTTTACCTTGTAACAAACGTGTTAAAATGTCACGGTTTTCTTCCAGTGTTCCGcc                                                                >  1:235467/1‑81 (MQ=255)           gctgctTCATGGGCGGCGTCGCCTTTACCTTGTAACAAACGTGTTAAAATGTCACGGTTTTCTTCCAGTGTTCCGCCTGcc                                                            <  1:235429/81‑1 (MQ=255)               ctTCATGGGCGGCGTCGCCTTTACCTTGTAACAAACGTGTTAAAATGTCACGGTTTTCTTCCAGTGTTCCGCCTGCCAg                                                          <  1:235430/79‑1 (MQ=255)                  cATGGGCGGCGTCGCCTTTACCTTGTAACAAACGTGTTAAAATGTCACGGTTTTCTTCCAGTGTTCCGCCTGCCAGTTGCt                                                     >  1:235462/1‑81 (MQ=255)                   aTGGGCGGCGTCGCCTTTACCTTGTAACAAACGTGTTAAAATGTCACGGTTTTCTTCTAGTGTTCCGCCTGCCAGTTGCTc                                                    >  1:235460/1‑81 (MQ=255)                     gggCGGCGTCGCCTTTACCTTGTAACAAACGTGTTAAAATGTCACGGTTTTCTTCCAGTGTTCCGCCTGCCAGTTGCTCCt                                                  >  1:235473/1‑81 (MQ=255)                      ggcggcGTCGCCTTTACCTTGTAACAAACGTGTTAAAATGTCACGGTTTTCTTCCAGTGTTCCGCCTGCCAGTTGCTCCtg                                                 >  2:235475/1‑81 (MQ=255)                        cggcgTCGCCTTTACCTTGTAACAAACGTGTTAAAATGTCACGGTTTTCTTCCAGTGTTCCGCCTGCCAGTTGCTCCtggt                                               <  1:235431/81‑1 (MQ=255)                         ggcgTCGCCTTTACCTTGTAACAAACGTGTTAAAATGTCACGGTTTTCTTCCAGTGTTCCGCCTGCCAGTTGCTCCtggtg                                              >  2:235463/1‑81 (MQ=255)                            gTCGCCTTTACCTTGTAACAAACGTGTTAAAATGTCACGGTTTTCTTCCAGTGTTCCGCCTGCCAGTTGCTCCtggtgg                                             >  2:235461/1‑79 (MQ=255)                                  tttACCTTGTAACAAACGTGTTAAAATGTCACGGTTTTCTTCCAGTGTTCCGCCTGCCAGTTGCTCCTGGTGGTAGGgtgt                                     >  1:235469/1‑81 (MQ=255)                                    tACCTTGTAACAAACGTGTTAAAATGTCACGGTTTTCTTCCAGTGTTCCGCCTGCCAGTTGCTCCTGGTGGTAGGGTGTc                                    <  2:235432/80‑1 (MQ=255)                                          gTAACAAACGTGTTAAAATGTCACGGTTTTCTTCCAGTGTTCCGCCTGCCAGTTGCTCCTGGTGGTAGGGTGTCAGGcc                               <  1:235433/79‑1 (MQ=255)                                             acgaaCGTGTTAAAATGTCACGGTTTTCTTCCAGTGTTCCGCCTGCCAGTTGCTCCTGGTGGTAGGGTGTCAGGCCAAAGt                          <  2:235434/78‑1 (MQ=255)                                                aaCGTGTTAAAATGTCACGGTTTTCTTCCAGTGTTCCGCCTGCCAGTTGCTCCTGGTGGTAGGGTGTCAGGCCAAAGtctt                       >  1:235464/1‑81 (MQ=255)                                                      ttAAAATGTCACGGGTTTCTTCCAGTGTTCCGCCTGCCAGTTGCTCCTGGTGGTAGGGTGTCAGGCCAAAGTCTTCTGCg                  <  2:235435/80‑1 (MQ=255)                                                         aaaTGTCACGGTTTTCTTCCAGTGTTCCGCCTGCCAGTTGCTCCTGGTGGTAGGGTGTCAGGCCAAAGTCTTCTGCGGTg               <  2:235436/80‑1 (MQ=255)                                                          aaTGTCACGGTTTTCTTCCAGTGTTCCGCCTGCCAGTTGCTCCTGGTGGTAGGGTGTCAGGCCAAAGTCTTCTGCGGTGAg             <  1:235437/81‑1 (MQ=255)                                                             gTCACGGTTTTCTTCCAGTGTTCCGCCTGCCAGTTGCTCCTGGTGGTAGGGTGTCAGGCCAAAGTCTTCTGCGGTGAGCt           >  1:235471/1‑80 (MQ=255)                                                               cACGGTTTTCTTCCAGTGTTCCGCCTGCCAGTTGCTCCTGGTGGTAGGGTGTCAGGCCAAAGTCTTCTGCGGTGAGCTGa         >  2:235474/1‑80 (MQ=255)                                                                aCGGTTTTCTTCCAGTGTTCCGCC‑GCCAGTTGCTCCTGGTGGTAGGGTGTCAGGCCAAAGTCTTCTGCGGTGAGCTGATAg      <  2:235438/81‑1 (MQ=255)                                                                   gTTTTCTTCCAGTGTTCCGCCTGCCAGTTGCTCCTGGTGGTAGGGTGTCAGGCCAAAGTCTTCTGCGGTGAGCTGATAGCt    <  1:235439/81‑1 (MQ=255)                                                                     cttcttcCAGTGTTCCGCCTGCCAGTCGCTCCTGGTGGTAGGGTGTCAGGCCAAAGTCCTCTGCGGTGAGCTGATAGCttt  >  2:235470/2‑81 (MQ=255)                                                                             |                                                                         CGCAGCGACGGCTGCTTCATGGGCGGCGTCGCCTTTACCTTGTAACAAACGTGTTAAAATGTCACGGTTTTCTTCCGGTGTTCCGCCTGCCAGTTGCTCCTGGTGGTAGGGTGTCAGGCCAAAGTCTTCTGCGGTGAGCTGATAGCTTT  >  NC_000913/1319915‑1320063

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑