Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,321,337	G→A	N16N (AAC→AAT)	trpD ←	anthranilate synthase subunit TrpD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,321,337	0	G	A	100.0%	121.7 / NA	35	N16N (AAC→AAT)	trpD	anthranilate synthase subunit TrpD
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (20/15);  total (20/15)

TCAATTAAGGTTTGCGCCGGAATATGGTTGCGGTAAATCACCACGTTATGCCCATTGCTGCGCAACTGATCTGCCAGGTTGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGTCTCCTGTGCATGATGCGCGGT  >  NC_000913/1321260‑1321413                                                                              |                                                                             tCAATTAAGGTTTGCGCCGGAATATGGTTGCGGTAAATCACCACGTTATGCCCATTGCTGCGCAACTGATCTGCCAGATTg                                                                           >  2:235716/1‑81 (MQ=255)             tGCGCCGGAATATGGTTGCGGTAAATCACCACGTTATGCCCATTGCTGCGCAACTGATCTGCCAGATTGTACGTAAAagag                                                               >  1:235705/1‑81 (MQ=255)                gcCGGAATATGGTTGCGGTAAATCACCACGTTATGCCCATTGCTGCGCAACTGATCTGCCAGATTGTACGTAAAAGAGTc                                                             >  2:235703/1‑80 (MQ=255)                      atatGGTTGCGGTAAATCACCACGTTATGCCCATTGCTGCGCAACTGATCTGCCAGATTGTACGTAAAAGAGTCGAtatta                                                      >  1:235712/1‑81 (MQ=255)                         tGGTTGCGGTAAATCACCACGTTATGCCCATTGCTGCGCAACTGATCTGCCAGATTGTACGTAAAAGAGTCGATATTATc                                                    <  2:235671/80‑1 (MQ=255)                              gCGGTAAATCACCACGTTATGCCCATTGCTGCGCAACTGATCTGCCAGATTGTACGTAAAAGAGTCGATATTATCGagcag                                              >  1:235719/1‑81 (MQ=255)                               cGGTAAATCACCACGTTATGCCCATTGCTGCGCAACTGATCTGCCAGATTGTACGTAAAAGAGTCGATATTATCGagcagc                                             <  1:235672/81‑1 (MQ=255)                                ggTAAATCACCACGTTATGCCCATTGCTGCGCAACTGATCTGCCAGATTGTACGTAAAAGAGTCGATATTATCGAgcagca                                            <  1:235673/81‑1 (MQ=255)                                     aTCACCACGTTATGCCCATTGCTGCGCAACTGATCTGCCAGATTGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATg                                       <  2:235674/81‑1 (MQ=255)                                      tCACCACGTTATGCCCATTGCTGCGCAACTGATCTGCCAGATTGTACGTAAAAGAGTCGATATTATCGGGCAGCAGAATGt                                      <  2:235675/81‑1 (MQ=255)                                        accacGTTATGCCCATTGCTGCGCAACTGATCTGCCAGATTGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGt                                      >  1:235709/1‑79 (MQ=255)                                        accacGTTATGCCCATTGCTGCGCAACTGATCTGCCAGATTGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTc                                     <  2:235676/80‑1 (MQ=255)                                            cGTTATGCCCATTGCTGCGCAACTGATCTGCCAGATTGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCa                                >  1:235706/1‑81 (MQ=255)                                             gTTATGCCCATTGCTGCGCAACTGATCTGCCAGATTGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCAt                               <  2:235677/81‑1 (MQ=255)                                             gTTATGCCCATTGCTGCGCAACTGATCTGCCAGATTGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCAt                               >  1:235722/1‑81 (MQ=255)                                              ttATGCCCATTGCTGCGCAACTGATCTGCCAGATTGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCAt                               >  2:235721/1‑80 (MQ=255)                                               tATGCCCATTGCTGCGCAACTGATCTGCCAGATTGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCAt                               >  1:235708/1‑79 (MQ=255)                                                  gCCCATTGCTGCGCAACTGATCTGCCAGATTGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGa                           >  2:235713/1‑80 (MQ=255)                                                   cccATTGCTGCGCAACTGATCTGCCAGATTGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGaaa                         <  1:235678/81‑1 (MQ=255)                                                   cccATTGCTGCGCAACTGATCTGCCAGATTGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGa                           >  2:235717/1‑79 (MQ=255)                                                     cATTGCTGCGCAACTGATCTGCCAGATTGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGt                       >  1:235729/1‑81 (MQ=255)                                                      aTTGCTGCGCAACTGATCTGCCAGATTGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGtc                      >  2:235718/1‑81 (MQ=255)                                                      aTTGCTGCGCAACTGATCTGCCAGATTGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGtc                      <  1:235679/81‑1 (MQ=255)                                                         gctgcGCAACTGATCTGCCAGATTGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGtctc                    <  2:235680/80‑1 (MQ=255)                                                         gctgcGCAACTGATCTGCCAGATTGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGTCTcc                   >  1:235714/1‑81 (MQ=255)                                                             cgcAACTGATCTGCCAGATTGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGTCTCCtgtg               <  1:235681/81‑1 (MQ=255)                                                             cgcAACTGATCTGCCAGATTGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGTCTCCtgtg               >  2:235715/1‑81 (MQ=255)                                                               cAACTGATCTGCCAGATTGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGTCTCCTGTGCa             <  2:235682/81‑1 (MQ=255)                                                                  cTGATCTGCCAGATTGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGTCTCCTGTGCatga          >  1:235733/1‑81 (MQ=255)                                                                    gATCTGCCAGATTGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGTCTCCTGTGCatgatg        >  2:235720/1‑81 (MQ=255)                                                                    gATCTGCCAGATTGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGTCTCCTGTGCatgatg        >  1:235699/1‑81 (MQ=255)                                                                     aTCTGCCAGATTGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGTCTCCTGTGCatgatg        <  2:235683/80‑1 (MQ=255)                                                                          ccAGATTGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGTCTCCTGTGCATGATgcgc     <  2:235685/78‑1 (MQ=255)                                                                          ccAGATTGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGTCTCCTGTGCATGATGCGCGGt  <  1:235686/81‑1 (MQ=255)                                                                          ccAGATTGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGTCTCCTGTGCATGATGCGCGGt  >  2:235736/1‑81 (MQ=255)                                                                              |                                                                             TCAATTAAGGTTTGCGCCGGAATATGGTTGCGGTAAATCACCACGTTATGCCCATTGCTGCGCAACTGATCTGCCAGGTTGTACGTAAAAGAGTCGATATTATCGAGCAGCAGAATGTCAGCCATCAGAAAGTCTCCTGTGCATGATGCGCGGT  >  NC_000913/1321260‑1321413

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑