Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,325,841	A→G	T366A (ACG→GCG)	yciQ →	DUF2207 domain‑containing protein YciQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,325,841	0	A	G	100.0%	82.7 / NA	24	T366A (ACG→GCG)	yciQ	DUF2207 domain‑containing protein YciQ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (7/17);  total (7/17)

ATGCGGTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTC  >  NC_000913/1325770‑1325917                                                                        |                                                                             atGCGGTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTGCGGGGAGAc                                                                     <  2:236362/81‑1 (MQ=255)               cccGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTGCGGGGAGACGCAACAGAGTCt                                                         <  2:236363/79‑1 (MQ=255)               cccGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTGCGGGGAGACGCAACAGAGTCTTa                                                       <  1:236364/81‑1 (MQ=255)                ccGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTGCGGGGAGACGCAACAGAGTCTTAc                                                      <  2:236365/81‑1 (MQ=255)                  gCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTGCGGGGAGACGCAACAGAGTCTTACGc                                                    <  2:236366/81‑1 (MQ=255)                              ttaCTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTGCGGGGAGACGCAACAGAGTCTTACGCAGGGCAttttt                                         <  1:236367/80‑1 (MQ=255)                              ttaCTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTGCGGGGAGACGCAACAGAGTCTTACGCAGGGCAttttt                                         <  2:236368/80‑1 (MQ=255)                                  tGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTGCGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGa                                    >  2:236394/1‑81 (MQ=255)                                    gAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTGCGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATg                                  <  1:236369/81‑1 (MQ=255)                                       gCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTGCGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAg                               <  2:236370/81‑1 (MQ=255)                                          ttATTTCGTAAAGGCGATAAGGTCGTTCTTGCGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGaaa                            >  1:236400/1‑81 (MQ=255)                                          ttATTTCGTAAAGGCGATAAGGTCGTTCTTGCGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGaaa                            >  1:236401/1‑81 (MQ=255)                                               tCGTAAAGGCGATAAGGTCGTTCTTGCGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAAtttt                        <  1:236371/80‑1 (MQ=255)                                                   aaaGGCGATAAGGTCGTTCTTGCGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTg                   >  1:236399/1‑81 (MQ=255)                                                     aGGCGATAAGGTCGTTCTTGCGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGcc                 >  1:236396/1‑81 (MQ=255)                                                      ggCGATAAGGTCGTTCTTGCGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCcg                <  2:236372/81‑1 (MQ=255)                                                       gCGATAAGGTCGTTCTTGCGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCcgc               <  1:236373/81‑1 (MQ=255)                                                       gCGATAAGGTCGTTCTTGCGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCcgc               <  2:236374/81‑1 (MQ=255)                                                        cGATAAGGTCGTTCTTGCGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCcgcg              <  2:236375/81‑1 (MQ=255)                                                         gATAAGGTCGTTCTTGCGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCcgcg              >  1:236395/1‑80 (MQ=255)                                                         gATAAGGTCGTTCTTGCGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCcgcg              >  1:236397/1‑80 (MQ=255)                                                           tAAGGTCGTTCTTGCGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTaa           <  1:236376/81‑1 (MQ=255)                                                             aGGTCGTTCTTGCGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTaaaa         <  2:236377/81‑1 (MQ=255)                                                                     cTTGCGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTc  <  2:236378/80‑1 (MQ=255)                                                                        |                                                                             ATGCGGTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTC  >  NC_000913/1325770‑1325917

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑