Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,332,269	T→C	Y408H (TAT→CAT)	topA →	DNA topoisomerase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,332,269	0	T	C	100.0%	93.2 / NA	27	Y408H (TAT→CAT)	topA	DNA topoisomerase 1
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (16/11);  total (16/11)

GAAGCAGATGCGCAGAAACTGTACCAGTTAATCTGGCGTCAGTTCGTTGCCTGCCAGATGACCCCAGCGAAATATGACTCCACGACGCTGACCGTTGGTGCGGGCGATTTCCGCCTGAAAGCACGCGGTCGTATTTTGCGTTTTGATGG  >  NC_000913/1332197‑1332345                                                                         |                                                                             gAAGCAGATGCGCAGAAACTGTACCAGTTAATCTGGCGTCAGTTCGTTGCCTGCCAGATGACCCCAGCGAAACATGACTc                                                                       >  2:237456/1‑80 (MQ=255)  aaGCAGATGCGCAGAAACTGTACCAGTTAATCTGGCGTCAGTTCGTTGCCTGCCAGATGACCCCAGCGAAACATGACTCCa                                                                     >  2:237455/1‑81 (MQ=255)   aGCAGATGCGCAGAAACTGTACCAGTTAATCTGGCGTCAGTTCGTTGCCTGCCAGATGACCCCAGCGAAACATGACTCCac                                                                    >  1:237446/1‑81 (MQ=255)          gcgcAGAAACTGTACCAGTTAATCTGGCGTCAGTTCGTTGCCTGCCAGATGACCCCAGCGAAACATGACTCCACGACGCt                                                              >  1:237450/1‑80 (MQ=255)             cAGAAACTGTACCAGTTAATCTGGCGTCAGTTCGTTGCCTGCCAGATGACCCCAGCGAAACATGACTCCACGACGCTGAcc                                                          <  1:237417/81‑1 (MQ=255)                 aaCTGTACCAGTTAATCTGGCGTCAGTTCGTTGCCTGCCAGATGACCCCAGCGAAACATGACTCCACGACGCTGACCGTTg                                                      <  2:237418/81‑1 (MQ=255)                     gTACCAGTTAATCTGGCGTCAGTTCGTTGCCTGCCAGATGACCCCAGCGAAACATGACTCCACGACGCTGACCGTTGGTGc                                                  >  2:237457/1‑81 (MQ=255)                             tAATCTGGCGTCAGTTCGTTGCCTGCCAGATGACCCCAGCGAAACATGACTCCACGACGCTGACCGTTGGTGCGGGCGAtt                                          >  2:237463/1‑81 (MQ=255)                              aaTCTGGCGTCAGTTCGTTGCCTGCCAGATGACCCCAGCGAAACATGACTCCACGACGCTGACCGTTGGTGCGGGCGAtt                                          <  2:237419/80‑1 (MQ=255)                                 cTGGCGTCAGTTCGTTGCCTGCCAGATGACCCCAGCGAAACATGACTCCACGACGCTGACCGTTGGTGCGGGCGATTTCCg                                      >  2:237451/1‑81 (MQ=255)                                    gCGTCAGTTCGTTGCCTGCCAGATGACCCCAGCGAAACATGACTCCACGACGCTGACCGTTGGTGCGGGCGGTTTcc                                       <  2:237421/77‑1 (MQ=255)                                    gCGTCAGTTCGTTGCCTGCCAGATGACCCCAGCGAAACATGACTCCACGACGCTGACCGTTGGTGCGGGCGATTTcc                                       <  1:237420/77‑1 (MQ=255)                                    gCGTCAGTTCGTTGCCTGCCAGATGACCCCAGCGAAACATGACTCCACGACGCTGACCGTTGGTGCGGGCGATTTcc                                       <  1:237422/77‑1 (MQ=255)                                        cAGTTCGTTGCCTGCCAGATGACCCCAGCGAAACATGACTCCACGACGCTGACCGTTGGTGCGGGCGATTTCCGCCTGa                                 >  2:237473/1‑79 (MQ=255)                                        cAGTTCGTTGCCTGCCAGATGACCCCAGCGAAACATGACTCCACGACGCTGACCGTTGGTGCGGGCGATTTCCGCCTGa                                 >  2:237442/1‑79 (MQ=255)                                         aGTTCGTTGCCTGCCAGATGACCCCAGCGAAACATGACTCCACGACGCTGACCGTTGGTGCGGGCGATTTCCGCCTGAAAg                              >  1:237465/1‑81 (MQ=255)                                              gTTGCCTGCCAGATGACCCCAGCGAAACATGACTCCACGACGCTGACCGTTGGTGCGGGCGATTTCCGCCTGAAAGCAcgc                         >  1:237466/1‑81 (MQ=255)                                                        aGATGACCCCAGCGAAACATGACTCCACGACGCTGACCGTTGGTGCGGGCGATTTCCGCCTGAAAGCACGCGGTCGTAttt               <  1:237423/81‑1 (MQ=255)                                                         gATGACCCCAGCGAAACATGACTCCACGACGCTGACCGTTGGTGCGGGCGATTTCCGCCTGAAAGCACGCGGTCGTAtttt              <  2:237424/81‑1 (MQ=255)                                                          aTGACCCCAGCGAAACATGACTCCACGACGCTGACCGTTGGTGCGGGCGATTTCCGCCTGAAAGCACGCGGTCGTATTTTg             >  1:237458/1‑81 (MQ=255)                                                           tGACCCCAGCGAAACATGACTCCACGACGCTGACCGTTGGTGCGGGCGATTTCCGCCTGAAAGCACGCGGTCGTATTTTGc            >  2:237459/1‑81 (MQ=255)                                                             aCCCCAGCGAAACATGACTCCACGACGCTGACCGTTGGTGCGGGCGATTTCCGCCTGAAAGCACGCGGTCGTATTTTGCg           <  2:237425/80‑1 (MQ=255)                                                             aCCCCAGCGAAACATGACTCCACGACGCTGACCGTTGGTGCGGGCGATTTACGCCTGAAAGCACGCGGTCGTATTTTGCGt          >  2:237467/1‑81 (MQ=255)                                                              ccccAGCGAAACATGACTCCACGACGCTGACCGTTGGTGCGGGCGATTTCCGCCTGAAAGCACGCGGTCGTATTTTGCGtt         <  2:237426/81‑1 (MQ=255)                                                                ccAGCGAAACATGACTCCACGACGCTGACCGTTGGTGCGGGCGATTTCCGCCTGAAAGCACGCGGTCGTATTTTGCGtttt       >  2:237471/1‑81 (MQ=255)                                                                     gAAACATGACTCCACGACGCTGACCGTTGGTGCGGGCGATTTCCGCCTGAAAGCACGCGGTCGTATTTTGCGTTTTGATgg  <  2:237427/81‑1 (MQ=255)                                                                     gAAACATGACTCCACGACGCTGACCGTTGGTGCGGGCGATTTCCGCCTGAAAGCACGCGGTCGTATTTTGCGTTTTGATgg  >  2:237460/1‑81 (MQ=255)                                                                         |                                                                             GAAGCAGATGCGCAGAAACTGTACCAGTTAATCTGGCGTCAGTTCGTTGCCTGCCAGATGACCCCAGCGAAATATGACTCCACGACGCTGACCGTTGGTGCGGGCGATTTCCGCCTGAAAGCACGCGGTCGTATTTTGCGTTTTGATGG  >  NC_000913/1332197‑1332345

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑