Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,338,149	A→T	G773G (GGA→GGT)	acnA →	aconitate hydratase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,338,149	0	A	T	100.0%	101.9 / NA	30	G773G (GGA→GGT)	acnA	aconitate hydratase 1
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (14/16);  total (14/16)

ATGATGCTGCGATGCGCTATAAGCAGGAGCAAACGCCGCTGGCGGTGATTGCCGGGAAAGAGTATGGATCAGGCTCCAGTCGTGACTGGGCGGCAAAAGGTCCGCGTCTGCTTGGTATTCGTGTGGTGATTGCCGAATCGTTT  >  NC_000913/1338082‑1338224                                                                    |                                                                            atgatgCTGCGATGCGCTATAAGCAGGAGCGAACGCCGCTGGCGGTGATTGCCGGGAAAGAGTATGGTTCAGGCTCCAGt                                                                 <  2:238264/80‑1 (MQ=255)  tgatgCTGCGATGCGCTATAAGCAGGAGCAAACGCCGCTGGCGGTGATTGCCGGGAAAGAGTATGGTTCAGGCTCCAGTc                                                                <  2:238265/80‑1 (MQ=255)    atgCTGCGATGCGCTATAAGCAGGAGCAAACGCCGCTGGCGGTGATTGCCGGGAAAGAGTATGGTTCAGGCTCCAGTCGt                                                              >  2:238302/1‑80 (MQ=255)          cGATGCGCTATAAGCAGGAGCAAACGCCGCTGGCGGTGATTGCCGGGAAAGAGTATGGTTCAGGCTCCAGTCGTGACTggg                                                       <  2:238266/81‑1 (MQ=255)          cGATGCGCTATAAGCAGGAGCAAACGCCGCTGGCGGTGATTGCCGGGAAAGAGTATGGTTCAGGCTCCAGTCGTGACTggg                                                       >  1:238301/1‑81 (MQ=255)           gATGCGCTATAAGCAGGAGCAAACGCCGCTGGCGGTGATTGCCGGGAAAGAGTATGGTTCAGGCTCCAGTCGTGACTGggc                                                      >  1:238303/1‑81 (MQ=255)            aTGCGCTATAAGCAGGAGCAAACGCCGCTGGCGGTGATTGCCGGGAAAGAGTATGGTTCAGGCTCCAGTCGTGACTGggcg                                                     >  1:238314/1‑81 (MQ=255)             tGCGCTATAAGCAGGAGCAAACGCCGCTGGCGGTGATTGCCGGGAAAGAGTATGGTTCAGGCTCCAGTCGTGACTGggcgg                                                    >  1:238310/1‑81 (MQ=255)              gcgcTATAAGCAGGAGCAAACGCCGCTGGCGGTGATTGCCGGGAAAGAGTATGGTTCAGGCTCCAGTCGTGACTGggcgg                                                    >  1:238319/1‑80 (MQ=255)               cgcTATAAGCAGGAGCAAACGCCGCTGGCGGTGATTGCCGGGAAAGAGTATGGTTCAGGCTCCAGTCGTGACTGggcggc                                                   <  1:238267/80‑1 (MQ=255)                      aGCAGGAGCAAACGCCGCTGGCGGTGATTGCCGGGAAAGAGTATGGTTCAGGCTCCAGTCGTGACTGGGCGGCAAAAGGt                                            <  2:238268/80‑1 (MQ=255)                       gCAGGAGCAAACGCCGCTGGCGGTGATTGCCGGGAAAGAGTATGGTTCAGGCTCCAGTCGTGACTGGGCGGCAAAAGGTcc                                          >  2:238305/1‑81 (MQ=255)                               aaaCGCCGCTGGCGGTGATTGCCGGGAAAGAGTATGGTTCAGGCTCCAGTCGTGACTGGGCGGCAAAAGGTCCGCGTCTGc                                  <  1:238269/81‑1 (MQ=255)                               aaaCGCCGCTGGCGGTGATTGCCGGGAAAGAGTATGGTTCAGGCTCCAGTCGTGACTGGGCGGCAAAAGGTCCGCGTCTGc                                  >  2:238316/1‑81 (MQ=255)                                aaCGCCGCTGGCGGTGATTGCCGGGAAAGAGTATGGTTCAGGCTCCAGTCGTGACTGGGCGGCAAAAGGTCCGCGTCTGCt                                 <  1:238270/81‑1 (MQ=255)                                 aCGCCGCTGGCGGTGATTGCCGGGAAAGAGTATGGTTCAGGCTCCAGTCGTGACTGGGCGGCAAAAGGTCCGCGTCTGCtt                                <  2:238271/81‑1 (MQ=255)                                 aCGCCGCTGGCGGTGATTGCCGGGAAAGAGTATGGTTCAGGCTCCAGTCGTGACTGGGCGGCAAAAGGTCCGCGTCTGCtt                                <  1:238272/81‑1 (MQ=255)                                  cgccgcTGGCGGTGATTGCCGGGAAAGAGTATGGTTCAGGCTCCAGTCGTGACTGGGCGGCAAAAGGTCCGCGTCTGCTTg                               >  2:238309/1‑81 (MQ=255)                                    ccgcTGGCGGTGATTGCCGGGAAAGAGTATGGTTCAGGCTCCAGTCGTGACTGGGCGGCAAAAGGTCCGCGTCTGCTTGGt                             <  2:238273/81‑1 (MQ=255)                                      gcTGGCGGTGATTGCCGGGAAAGAGTATGGTTCAGGCTCCAGTCGTGACTGGGCGGCAAAAGGTCCGCGTCTGCTTGGTAt                           >  1:238313/1‑81 (MQ=255)                                       cTGGCGGTGATTGCCGGGAAAGAGTATGGTTCAGGCTCCAGTCGTGACTGGGCGGCAAAAGGTCCGCGTCTGCTTGGTAtt                          >  2:238325/1‑81 (MQ=255)                                          gCGGTGATTGCCGGGAAAGAGTATGGTTCGGGCTCCAGTCGTGACTGGGCGGCAAAAGGTCCGCGTCTGCTTGGTATTc                         <  2:238274/79‑1 (MQ=255)                                              tGATTGCCGGGAAAGAGTATGGTTCAGTCTCCAGTCGTGACTGGGCGGCAAAAGGTCCGCGTCTGCTTGGTATTCGTgtgg                   >  1:238294/1‑81 (MQ=255)                                               gATTGCCGGGAAAGAGTATGGTTCAGGCTCCAGTCGTGACTGGGCGGCAAAAGGTCCGCGTCTGCTTGGTATTCGTgtggt                  >  2:238311/1‑81 (MQ=255)                                                aTTGCCGGGAAAGAGTATGGTTCAGGCTCCAGTCGTGACTGGGCGGCAAAAGGTCCGCGTCTGCTTGGTATTCGTgtggtg                 <  1:238275/81‑1 (MQ=255)                                                    ccGGGAAAGAGTATGGTTCAGGCTCCAGTCGTGACTGGGCGGCAAAAGGTCCGCGTCTGCTTGGTATTCGTGTGGTGAtt              <  1:238276/80‑1 (MQ=255)                                                          aaGAGTATGGTTCAGGCTCCAGTCGTGACTGGGCGGCAAAAGGTCCGCGTCTGCTTGGTGTTCGTGTGGTGATTGCCGAAt       <  2:238277/81‑1 (MQ=255)                                                           agagTATGGTTCAGGCTCCAGTCGTGACTGGGCGGCAAAAGGTCCGCGTCTGCTTGGTATTCGTGTGGTGATTGCCGAATc      <  1:238278/81‑1 (MQ=255)                                                              gTATGGTTCAGGCTCCAGTCGTGACTGGGCGGCAAAAGGTCCGCGTCTGCTTGGTATTCGTGTGGTGATTGCCGAATCGtt   <  1:238279/81‑1 (MQ=255)                                                               tATGGTTCAGGCTCCAGTCGTGACTGGGCGGCAAAAGGTCCGCGTCTGCTTGGTATTCGTGTGGTGATTGCCGAATCGttt  >  2:238312/1‑81 (MQ=255)                                                                    |                                                                            ATGATGCTGCGATGCGCTATAAGCAGGAGCAAACGCCGCTGGCGGTGATTGCCGGGAAAGAGTATGGATCAGGCTCCAGTCGTGACTGGGCGGCAAAAGGTCCGCGTCTGCTTGGTATTCGTGTGGTGATTGCCGAATCGTTT  >  NC_000913/1338082‑1338224

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑