Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,353,437	A→T	T64T (ACT→ACA)	sapD ←	putrescine ABC exporter ATP binding protein SapD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,353,437	0	A	T	100.0%	97.7 / NA	28	T64T (ACT→ACA)	sapD	putrescine ABC exporter ATP binding protein SapD
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (11/17);  total (11/17)

TTATGGCCAACCAGTTTGCGCCGTTCGCGTGCGGAGAGACGCAGCAAATCGATATCATCAAAACGCATACGGTCAGCAGTAACACGCCAGTTATCTTTATTCACCCCACAAATCGCTTTCGCAATCAAACTCTTGCCGGAACCGGATTCACCAA  >  NC_000913/1353360‑1353513                                                                              |                                                                             ttATGGCCAACCAGTTTGCGCCGTTCGCGTGCGGAGAGACGCAGCAAATCGATATCATCAAAACGCATACGGTCAGCTGTa                                                                           <  2:240737/81‑1 (MQ=255)   aTGGCCAACCAGTTTGCGCCGTTCGCGTGCGGAGAGACGCAGCAAATCGATATCATCAAAACGCATACGGTCAGCTGTaa                                                                          >  2:240769/1‑80 (MQ=255)      gCCAACCAGTTTGCGCCGTTCGCGTGCGGAGAGACGCAGCAAATCGATATCATCAAAACGCATACGGTCAGCTGTAACACg                                                                      >  2:240772/1‑81 (MQ=255)        cAACCAGTTTGCGCCGTTCGCGTGCGGAGAGACGCAGCAAATCGATATCATCAAAACGCATACGGTCAGCTGTAACACGcc                                                                    <  2:240738/81‑1 (MQ=255)         aaCCAGTTTGCGCCGTTCGCGTGCGGAGAGACGCAGCAAATCGATATCATCAAAACGCATACGGTCAGCTGTAACACGCCa                                                                   <  2:240739/81‑1 (MQ=255)                ttGCGCCGTTCGCGTGCGGAGAGACGCAGCAAATCGATATCATCAAAACGCATACGGTCAGCTGTAACACGCCAGTTATCt                                                            >  2:240785/1‑81 (MQ=255)                   cgcCGTTCGCGTGCGGAGAGACGCAGCAAATCGATATCATCAAAACGCATACGGTCAGCTGTAACACGCCAGTTATCttt                                                          <  1:240740/80‑1 (MQ=255)                        ttCGCGTGCGGAGAGACGCAGCAAATCGATATCATCAAAACGCATACGGTCAGCTGTAACACGCCAGTTATCTTTATTCAc                                                    >  2:240775/1‑81 (MQ=255)                        ttCGCGTGCGGAGAGACGCAGCAAATCGATATCATCAAAACGCATACGGTCAGCTGTAACACGCCAGTTATCTTTATTCAc                                                    <  2:240741/81‑1 (MQ=255)                          cgcgTGCGGAGAGACGCAGCAAATCGATATCATCAAAACGCATACGGTCAGCTGTAACACGCCAGTTATCTTTATTCAcc                                                   <  2:240742/80‑1 (MQ=255)                            cgTGCGGAGAGACGCAGCAAATCGATATCATCAAAACGCATACGGTCAGCTGTAACACGCCAGTTATCTTTATTCACCCca                                                >  2:240787/1‑81 (MQ=255)                                cGGAGAGACGCAGCAAATCGATATCATCAAAACGCATACGGTCAGCTGTAACACGCCAGTTATCTTTATTCACCCCACaa                                             <  1:240743/80‑1 (MQ=255)                                 ggAGAGACGCAGCAAATCGATATCATCAAAACGCATACGGTCAGCTGTAACACGCCAGTTATCTTTATTCACCCCACAAAt                                           <  1:240744/81‑1 (MQ=255)                                 ggAGAGACGCAGCAAATCGATATCATCAAAACGCATACGGTCAGCTGTAACACGCCAGTTATCTTTATTCACCCCACAAAt                                           <  1:240745/81‑1 (MQ=255)                                         gcagcaAATCGATATCATCAAAACGCATACGGTCAGCTGTAACACGCCAGTTATCTTTATTCACCCCACAAATCGCTTTCg                                   <  1:240746/81‑1 (MQ=255)                                               aaTCGATATCATCAAAACGCATACGGTCAGCTGTAACACGCCAGTTATCTTTATTCACCCCACAAATCGCTTTCGCAATc                              >  2:240781/1‑80 (MQ=255)                                               aaTCGATATCATCAAAACGCATACGGTCAGCTGTAACACGCCAGTTATCTTTATTCACCCCACAAATCGCTTTCGCAATCa                             <  2:240747/81‑1 (MQ=255)                                                     tatCATCAAAACGCATACGGTCAGCTGTAACACGCCAGTTATCTTTATTCACCCCACAAATCGCTTTCGCAATCAAActct                       <  1:240748/81‑1 (MQ=255)                                                      atcatcAAAACGCATACGGTCAGCTGTAACACGCCAGTTATCTTTATTCACCCCACAAATCGCTTTCGCAATCAAACTCtt                      >  1:240793/1‑81 (MQ=255)                                                       tcatcaAAACGCATACGGTCAGCTGTAACACGCCAGTTATCTTTATTCACCCCACAAATCGCTTTCGCAATCAAACTCtt                      <  2:240749/80‑1 (MQ=255)                                                             aaaCGCATACGGTCAGCTGTAACACGCCAGTTATCTTTATTCACCCCACAAATCGCTTTCGCAATCAAACTCTTGCCGGaa               <  1:240750/81‑1 (MQ=255)                                                             aaaCGCATACGGTCAGCTGTAACACGCCAGTTATCTTTATTCACCCCACAAATCGCTTTCGCAATCAAACTCTTGCCGGaa               >  1:240779/1‑81 (MQ=255)                                                              aaCGCATACGGTCAGCTGTAACACGCCAGTTATCTTTATTCACCCCACAAATCGCTTTCGCAATCAAACTCTTGCCGGAAc              <  2:240752/81‑1 (MQ=255)                                                              aaCGCATACGGTCAGCTGTAACACGCCAGTTATCTTTATTCACCCCACAAATCGCTTTCGCAATCAAACTCTTGCCGGAAc              <  2:240753/81‑1 (MQ=255)                                                              aaCGCATACGGTCAGCTGTAACACGCCAGTTATCTTTATTCACCCCACAAATCGCTTTCGCAATCAAACTCTTGCCGGAAc              <  2:240751/81‑1 (MQ=255)                                                                 gCATACGGTCAGCTGTAACACGCCAGTTATCTTTATTCACCCCACAAATCGCTTTCGCAATCAAACTCTTGCCGGAACCg            >  2:240782/1‑80 (MQ=255)                                                                  cATACGGTCAGCTGTAACACGCCAGTTATCTTTATTCACCCCACAAATCGCTTTCGCAATCAAACTCTTGCCGGAACCGGa          >  2:240783/1‑81 (MQ=255)                                                                       ggTCAGCTGTAACACGCCAGTTATCTTTATTCACCCCACAAATCGCTTTCGCAATCAAACTCTTGCCGGAACCGGATTCAc     >  1:240794/1‑81 (MQ=255)                                                                          cagctgTAACACGCCAGTTATCTTTATTCACCCCACAAATCGCTTTCGCAATCAAACTCTTGCCGGAACCGGATTCACcaa  >  1:240786/1‑81 (MQ=255)                                                                              |                                                                             TTATGGCCAACCAGTTTGCGCCGTTCGCGTGCGGAGAGACGCAGCAAATCGATATCATCAAAACGCATACGGTCAGCAGTAACACGCCAGTTATCTTTATTCACCCCACAAATCGCTTTCGCAATCAAACTCTTGCCGGAACCGGATTCACCAA  >  NC_000913/1353360‑1353513

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑