Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,371,484	T→C	I423I (ATT→ATC)	ycjM →	glucosylglycerate phosphorylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,371,484	0	T	C	100.0%	50.3 / NA	15	I423I (ATT→ATC)	ycjM	glucosylglycerate phosphorylase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (3/12);  total (3/12)

CGCCAGGTTTATCCTTGCCCATGCGATTTTGTTAAGTTTCCCCGGTGTGCCAGCGATATATATTCAAAGTATTCTTGGCTCGCGTAATGATTACGCAGGTGTCGAAAAACTCGGATATAACCGTGCGATTAACCGTAAA  >  NC_000913/1371421‑1371559                                                                |                                                                            cgcCAGGTTTATCCTTGCCCATGCGATTTTGTTAAGTTTCCCCGGTGTGCCAGCGATATATATCCTAAGTATTCTTGGCt                                                             <  2:243872/80‑1 (MQ=255)     aGGTTTATCCTTGCCCATGCGATTTTGTTAAGTTTCCCCGGTGTGCCAGCGATATATATCCAAAGTATTCTTGGCTCGCGt                                                        <  2:243873/81‑1 (MQ=255)        tttATCCTTGCCCATGCGATTTTGTTAAGTTTCCCCGGTGTGCCAGCGATATATATCCAAAGTATTCTTGGCTCGCGTAAt                                                     >  1:243895/1‑81 (MQ=255)          tATCCTTGCCCATGCGATTTTGTTAAGTTTCCCCGGTGTGCCAGCGATATATATCCAAAGTATTCTTGGCTCGCGTAATg                                                    <  2:243874/80‑1 (MQ=255)            tCCTTGCCCATGCGATTTTGTTAAGTTTCCCCGGTGTGCCAGCGATATATATCCAAAGTATTCTTGGCTCGCGTAATGAt                                                  <  1:243875/80‑1 (MQ=255)              cTTGCCCATGCGATTTTGTTAAGTTTCCCCGGTGTGCCAGCGATATATATCCAAAGTATTCTTGGCTCGCGTAATGATTAc                                               <  2:243876/81‑1 (MQ=255)                     aTGCGATTTTGTTAAGTTTCCCCGGTGTGCCAGCGATATATATCCAAAGTATTCTTGGCTCGCGTAATGATTACGCAGgtg                                        >  2:243900/1‑81 (MQ=255)                        cGATTTTGTTAAGTTTCCCCGGTGTGCCAGCGATATATATCCAAAGTATTCTTGGCTCGCGTAATGATTACGCAGGTGTc                                      <  2:243877/80‑1 (MQ=255)                             ttGTTAAGTTTCCCCGGTGTGCCAGCGATATATATCCAAAGTATTCTTGGCTCGCGTAATGATTACGCAGGTGTCGaaaa                                 <  1:243878/80‑1 (MQ=255)                              tGTTAAGTTTCCCCGGTGTGCCAGCGATATATATCCAAAGTATTCTTGGCTCGCGTAATGATTACGCAGGTGTCGaaaaa                                <  2:243879/80‑1 (MQ=255)                                   aGTTTCCCCGGTGTGCCAGCGATATATATCCAAAGTATTCTTGGCTCGCGTAATGATTACGCAGGTGTCGAAAAACTCGGa                          >  2:243899/1‑81 (MQ=255)                                         cccGGTGTGCCAGCGATATATATCCAAAGTATTCTTGGCTCGCGTAATGATTACGCAGGTGTCGAAAAACTCGGATATaa                     <  1:243880/80‑1 (MQ=255)                                           cGGTGTGCCAGCGATATATATCCAAAGTATTCTTGGCTCGCGTAATGATTACGCAGGTGTCGAAAAACTCGGATATAACCg                  <  2:243881/81‑1 (MQ=255)                                                     gCGATATATATCCAAAGTATTCTTGGCTCGCGTAATGATTACGCAGGTGTCGAAAAACTCGGATATAACCGTGCGATTAAc        <  2:243882/81‑1 (MQ=255)                                                           tatatCCAAAGTATTCTTGGCTCGCGTAATGATTACGCAGGTGTCGAAAAACTCGGATATAACCGTGCGATTAACCGTaaa  <  1:243883/81‑1 (MQ=255)                                                                |                                                                            CGCCAGGTTTATCCTTGCCCATGCGATTTTGTTAAGTTTCCCCGGTGTGCCAGCGATATATATTCAAAGTATTCTTGGCTCGCGTAATGATTACGCAGGTGTCGAAAAACTCGGATATAACCGTGCGATTAACCGTAAA  >  NC_000913/1371421‑1371559

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑