Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,385,122	G→A	A336T (GCC→ACC)	ycjX →	DUF463 domain‑containing protein YcjX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,385,122	0	G	A	100.0%	90.0 / NA	26	A336T (GCC→ACC)	ycjX	DUF463 domain‑containing protein YcjX
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (16/10);  total (16/10)

ATGCAAAGTTTTCACTACGGGCAGCGTACCCTGTTCCGGCGTTTGTTTTCGCCGGTTATCGATAAGCTATTGTTTGCTGCCACTAAAGCGGACCATGTGACCATCGATCAGCACGCTAATATGGTTTCATTACTGCAACAACTGATTCAGGATGC  >  NC_000913/1385044‑1385198                                                                               |                                                                             aTGCAAAGTTTTCACTACGGGCAGCGTACCCTGTTCCGGCGTTTGTTTTCGCCGGTTATCGATAAGCTATTGTTTGCTAcc                                                                            <  2:246340/81‑1 (MQ=255)   gCAAAGTTTTCACTACGGGCAGCGTACCCTGTTCCGGCGTTTGTTTTCGCCGGTTATCGATAAGCTATTGTTTGCTACCAc                                                                          >  1:246367/1‑81 (MQ=255)     aaaGTTTTCACTACGGGCAGCGTACCCTGTTCCGGCGTTTGTTTTCGCCGGTTATCGATAAGCTATTGTTTGCTACCACt                                                                         >  2:246381/1‑80 (MQ=255)      aaGTTTTCACTACGGGCAGCGTACCCTGTTCCGGCGTTTGTTTTCGCCGGTTATCGATAAGCTATTGTTTGCTACCACTaa                                                                       <  1:246341/81‑1 (MQ=255)       aGTTTTCACTACGGGCAGCGTACCCTGTTCCGGCGTTTGTTTTCGCCGGTTATCGATAAGCTATTGTTTGCTACCACTa                                                                        >  2:246380/1‑79 (MQ=255)              aCTACGGGCAGCGTACCCTGTTCCGGCGTTTGTTTTCGCCGGTTATCGATAAGCTATTGTTTGCTACCACTAAAGCGGAc                                                                >  2:246372/1‑80 (MQ=255)                  cGGGCAGCGTACCCTGTTCCGGCGTTTGTTTTCGCCGGTTATCGATAAGCTATTGTTTGCTACCACTAAAGCGGACCAtgt                                                           <  1:246343/81‑1 (MQ=255)                  cGGGCAGCGTACCCTGTTCCGGCGTTTGTTTTCGCCGGTTATCGATAAGCTATTGTTTGCTACCACTAAAGCGGACCAtg                                                            <  2:246342/80‑1 (MQ=255)                       aGCGTACCCTGTTCCGGCGTTTGTTTTCGCCGGTTATCGATAAGCTATTGTTTGCTACCACTAAAGCGGACCATGTGACCa                                                      >  1:246370/1‑81 (MQ=255)                          gTACCCTGTTCCGGCGTTTGTTTTCGCCGGTTATCGATAAGCTATTGTTTGCTACCACTAAAGCGGACCATGTGACCATCg                                                   <  2:246344/81‑1 (MQ=255)                            aCCCTGTTCCGGCGTTTGTTTTCGCCGGTTATCGATAAGCTATTGTTTGCTACCACTAAAGCGGACCATGTGACCATCGAt                                                 >  1:246387/1‑81 (MQ=255)                             cccTGTTCCGGCGTTTGTTTTCGCCGGTTATCGATAAGCTATTGTTTGCTACCACTAAAGCGGACCATGTGACCATCGAt                                                 <  1:246345/80‑1 (MQ=255)                               cTGTTCCGGCGTTTGTTTTCGCCGGTTATCGATAAGCTATTGTTTGCTACCACTAAAGCGGACCATGTGACCATCGATCAg                                              >  1:246376/1‑81 (MQ=255)                               cTGTTCCGGCGTTTGTTTTCGCCGGTTATCGATAAGCTATTGTTTGCTACCACTAAAGCGGACCATGTGACCATCGATCAg                                              >  1:246393/1‑81 (MQ=255)                                  ttCCGGCGTTTGTTTTCGCCGGTTATCGATAAGCTATTGTTTGCTACCACTAAAGCGGACCATGTGACCATCGATCAGCAc                                           <  1:246346/81‑1 (MQ=255)                                     cGGCGTTTGTTTTCGCCGGTTATCGATAAGCTATTGTTTGCTACCACTAAAGCGGACCATGTGACCATCGATCAGCACGc                                         <  2:246347/80‑1 (MQ=255)                                        cGTTTGTTTTCGCCGGTTATCGATAAGCTATTGTTTGCTACCACTAAAGCGGACCATGTGACCATCGATCAGCACGCTaa                                      >  1:246373/1‑80 (MQ=255)                                                  cGCCGGTTATCGATAAGCTATTGTTTGCTACCACTAAAGCGGACCATGTGACCATCGATCAGCACGCTAATATGGTTTCAt                           >  2:246395/1‑81 (MQ=255)                                                        ttATCGATAAGCTATTGTTTGCTACCACTAAAGCGGACCATGTGACCATCGATCAGCACGCTAATATGGTTTCATTACTGc                     <  2:246348/81‑1 (MQ=255)                                                             gATAAGCTATTGTTTGCTACCACTAAAGCGGACCATGTGACCATCGATCAGCACGCTAATATGGTTTCATTACTGcaacaa                >  1:246369/1‑81 (MQ=255)                                                                 aGCTATTGTTTGCTACCACTAAAGCGGACCATGTGACCATCGATCAGCACGCTAATATGGTTTCATTACTGCAACAACTGa            >  1:246403/1‑81 (MQ=255)                                                                    tATTGTTTGCTACCACTAAAGCGGACCATGTGACCATCGATCAGCACGCTAATATGGTTTCATTACTGCAACAACTGAtt          >  1:246399/1‑80 (MQ=255)                                                                         tttGCTACCACTAAAGCGGACCATGTGACCATCGATCAGCACGCTAATATGGTTTCATTACTGCAACAACTGATTCAGGa     <  1:246349/80‑1 (MQ=255)                                                                         tttGCTACCACTAAAGCGGACCATGTGACCATCGATCAGCACGCTAATATGGTTTCATTACTGCAACAACTGATTCAGGAt    >  1:246390/1‑81 (MQ=255)                                                                         tttGCTACCACTAAAGCGGACCATGTGACCATCGATCAGCACGCTAATATGGTTTCATTACTGCAACAACTGATTCAGGAt    >  1:246382/1‑81 (MQ=255)                                                                          ttGCTACCACTAAAGCGGACCATGTGACCATCGATCAGCACGCTAATATGGTTTCATTACTGCAACAACTGATTCAGGATg   >  1:246374/1‑81 (MQ=255)                                                                           tgctacCACTAAAGCGGACCATGTGACCATCGATCAGCACGCTAATATGGTTTCATTACTGCAACAACTGATTCAGGATGc  >  2:246389/1‑81 (MQ=255)                                                                               |                                                                             ATGCAAAGTTTTCACTACGGGCAGCGTACCCTGTTCCGGCGTTTGTTTTCGCCGGTTATCGATAAGCTATTGTTTGCTGCCACTAAAGCGGACCATGTGACCATCGATCAGCACGCTAATATGGTTTCATTACTGCAACAACTGATTCAGGATGC  >  NC_000913/1385044‑1385198

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑