Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	94,873	T→A	L75Q (CTG→CAG)	murF →	D‑alanyl‑D‑alanine‑adding enzyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	94,873	0	T	A	100.0%	221.7 / NA	62	L75Q (CTG→CAG)	murF	D‑alanyl‑D‑alanine‑adding enzyme
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (29/33);  total (29/33)

ATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTACTGGTTAGCCGTCCGCTGGACATCGACCTGCCGCAGTTAATCGTCAAGGATACGCGTCTGGCGTTTGGTGAACTGGCTGCATGGGTTCGCCAGCAAGTTCCGGCGC  >  NC_000913/94795‑94950                                                                               |                                                                              aTGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTACTGGTTAGCCGTCCGCTGGACATCGACCAGc                                                                             >  1:19317/1‑81 (MQ=255)  tGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTACTGGTTAGCCGTCCGCTGGACATCGACCAGcc                                                                            <  1:19252/81‑1 (MQ=255)    cccACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTACTGGTTAGCCGTCCGCTGGACATCGACCAGCCg                                                                           >  2:19333/1‑80 (MQ=255)    cccACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTACTGGTTAGCCGTCCGCTGGACATCGACCAGCCGc                                                                          <  1:19253/81‑1 (MQ=255)     ccACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTACTGGTTAGCCGTCCGCTGGACATCGACCAGCCGCa                                                                         <  1:19254/81‑1 (MQ=255)      cACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTACTGGTTAGCCGTCCGCTGGACATCGACCAGCCGCAg                                                                        <  2:19255/81‑1 (MQ=255)       aCGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTACTGGTTAGCCGTCCGCTGGACATCGACCAGCCGCAGt                                                                       <  2:19256/81‑1 (MQ=255)         gATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTACTGGTTAGCCGTCCGCTGGACATCGACCAGCCGCAGtt                                                                      >  2:19326/1‑80 (MQ=255)         gATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTACTGGTTAGCCGTCCGCTGGACATCGACCAGCCGCAGTTa                                                                     <  2:19257/81‑1 (MQ=255)          aTTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTACTGGTTAGCCGTCCGCTGGACATCGACCAGCCGCAGTTaa                                                                    <  1:19258/81‑1 (MQ=255)          aTTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTACTGGTTAGCCGTCCGCTGGACATCGACCAGCCGCAGTTaa                                                                    >  2:19313/1‑81 (MQ=255)             ttGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTACTGGTTAGCCGTCCGCTGGACATCGACCAGCCGCAGTTAATCg                                                                 >  2:19354/1‑81 (MQ=255)             ttGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTACTGGTTAGCCGTCCGCTGGACATCGACCAGCCGCAGTTAATCg                                                                 >  2:19315/1‑81 (MQ=255)             ttGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTACTGGTTAGCCGTCCGCTGGACATCGACCAGCCGCAGTTAATCg                                                                 >  1:19322/1‑81 (MQ=255)             ttGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTACTGGTTAGCCGTCCGCTGGACATCGACCAGCCGCAGTTAATCg                                                                 >  1:19312/1‑81 (MQ=255)              tGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTACTGGTTAGCCGTCCGCTGGACATCGACCAGCCGCAGTTAATCg                                                                 <  1:19259/80‑1 (MQ=255)               gCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTACTGGTTAGCCGTCCGCTGGACATCGACCAGCCGCAGTTAATCGt                                                                >  2:19320/1‑80 (MQ=255)                ccGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTACTGGTTAGCCGTCCGCTGGACATCGACCAGCCGCAGTTAATCGTc                                                               <  2:19261/80‑1 (MQ=255)                ccGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTACTGGTTAGCCGTCCGCTGGACATCGACCAGCCGCAGTTAATCGTc                                                               <  2:19260/80‑1 (MQ=255)                 cGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTACTGGTTAGCCGTCCGCTGGACATCGACCAGCCGCAGTTAATCGTCaa                                                             <  1:19262/81‑1 (MQ=255)                  gACCAGGCGAAAGCTGGCGGCGCAGGCGCACTACTGGTTAGCCGTCCGCTGGACATCGACCAGCCGCAGTTAATCGTCAAg                                                            <  2:19263/81‑1 (MQ=255)                     cAGGCGAAAGCTGGCGGCGCAGGCGCACTACTGGTTAGCCGTCCGCTGGACATCGACCAGCCGCAGTTAATCGTCAAGGa                                                          >  2:19327/1‑80 (MQ=255)                      aGGCGAAAGCTGGCGGCGCAGGCGCACTACTGGTTAGCCGTCCGCTGGACATCGACCAGCCGCAGTTAATCGTCAAGGATa                                                        >  1:19310/1‑81 (MQ=255)                         cGAAAGCTGGCGGCGCAGGCGCACTACTGGTTAGCCGTCCGCTGGACATCGACCAGCCGCAGTTAATCGTCAAGGATAcgc                                                     <  1:19264/81‑1 (MQ=255)                         cGAAAGCTGGCGGCGCAGGCGCACTACTGGTTAGCCGTCCGCTGGACATCGACCAGCCGCAGTTAATCGTCAAGGATAcgc                                                     <  1:19265/81‑1 (MQ=255)                          gAAAGCTGGCGGCGCAGGCGCACTACTGGTTAGCCGTCCGCTGGACATCGACCAGCCGCAGTTAATCGTCAAGGATAcgcg                                                    >  2:19349/1‑81 (MQ=255)                          gAAAGCTGGCGGCGCAGGCGCACTACTGGTTAGCCGTCCGCTGGACATCGACCAGCCGCAGTTAATCGTCAAGGATAcgcg                                                    >  1:19339/1‑81 (MQ=255)                          gAAAGCTGGCGGCGCAGGCGCACTACTGGTTAGCCGTCCGCTGGACATCGACCAGCCGCAGTTAATCGTCAAGGATAcgcg                                                    >  2:19316/1‑81 (MQ=255)                           aaaGCTGGCGGCGCAGGCGCACTACTGGTTAGCCGTCCGCTGGACATCGACCAGCCGCAGTTAATCGTCAAGGATACGCGt                                                   >  1:19389/1‑81 (MQ=255)                             aGCTGGCGGCGCAGGCGCACTACTGGTTAGCCGTCCGCTGGACATCGACCAGCCGCAGTTAATCGTCAAGGATACGCGTCt                                                 >  1:19325/1‑81 (MQ=255)                              gCTGGCGGCGCAGGCGCACTACTGGTTAGCCGTCCGCTGGACATCGACCAGCCGCAGTTAATCGTCAAGGATACGCGTCTg                                                >  2:19346/1‑81 (MQ=255)                               cTGGCGGCGCAGGCGCACTACTGGTTAGCCGTCCGCTGGACATCGACCAGCCGCAGTTAATCGTCAAGGATACGCGTCTgg                                               <  1:19266/81‑1 (MQ=255)                                    ggcgcaggcgcaCTACTGGTTAGCCGTCCGCTGGACATCGACCAGCCGCAGTTAATCGTCAAGGATACGCGTCTGGCGttt                                          >  2:19335/1‑81 (MQ=255)                                         aggcgcaCTACTGGTTAGCCGTCCGCTGGACATCGACCAGCCGCAGTTAATCGTCAAGGATACGCGTCTGGCGTTTGGTGa                                     <  2:19267/81‑1 (MQ=255)                                           gcgcaCTACTGGTTAGCCGTCCGCTGGACATCGACCAGCCGCAGTTAATTGTCAAGGATACGCGTCTGGCGTTTGGTGAAc                                   >  2:19341/1‑81 (MQ=255)                                           gcgcaCTACTGGTTAGCCGTCCGCTGGACATCGACCAGCCGCAGTTAATCGTCAAGGATACGCGTCTGGCGTTTGGTGAAc                                   >  2:19321/1‑81 (MQ=255)                                             gcaCTACTGGTTAGCCGTCCGCTGGACATCGACCAGCCGCAGTTAATCGTCAAGGATACGCGTCTGGCGTTTGGTGAACTg                                 <  2:19268/81‑1 (MQ=255)                                             gcaCTACTGGTTAGCCGTCCGCTGGACATCGACCAGCCGCAGTTAATCGTCAAGGATACGCGTCTGGCGTTTGGTGAACTg                                 >  1:19336/1‑81 (MQ=255)                                                   ctGGTTAGCCGTCCGCTGGACATCGACCAGCCGCAGTTAATCGTCAAGGATACGCGTCTGGCGTTTGGTGAACTGGCTGCa                           >  1:19353/1‑81 (MQ=255)                                                    tGGTTAGCCGTCCGCTGGACATCGACCAGCCGCAGTTAATCGTCAAGGATACGCGTCTGGCGTTTGGTGAACTGGCTGCAt                          >  1:19340/1‑81 (MQ=255)                                                      gTTAGCCGTCCGCTGGACATCGACCAGCCGCAGTTAATCGTCAAGGATACGCGTCTGGCGTTTGGTGAACTGGCTGCATgg                        <  2:19269/81‑1 (MQ=255)                                                          gCCGTCCGCTGGACATCGACCAGCCGCAGTTAATCGTCAAGGATACGCGTCTGGCGTTTGGTGAACTGGTTGCATGGGTTc                    <  1:19272/81‑1 (MQ=255)                                                          gCCGTCCGCTGGACATCGACCAGCCGCAGTTAATCGTCAAGGATACGCGTCTGGCGTTTGGTGAACTGGCTGCATGGGtt                     <  1:19270/80‑1 (MQ=255)                                                          gCCGTCCGCTGGACATCGACCAGCCGCAGTTAATCGTCAAGGATACGCGTCTGGCGTTTGGTGAACTGGCTGCATGGGTTc                    <  2:19273/81‑1 (MQ=255)                                                          gCCGTCCGCTGGACATCGACCAGCCGCAGTTAATCGTCAAGGATACGCGTCTGGCGTTTGGTGAACTGGCTGCATGGGTTc                    <  2:19271/81‑1 (MQ=255)                                                           ccGTCCGCTGGACATCGACCAGCCGCAGTTAATCGTCAAGGATACGCGTCTGGCGTTTGGTGAACTGGCTGCATGGGTTCg                   <  2:19274/81‑1 (MQ=255)                                                              tCCGCTGGACATCGACCAGCCGCAGTTAATCGTCAAGGATACGCGTCTGGCGTTTGGTGAACTGGCTGCATGGGTTCGCCa                <  2:19275/81‑1 (MQ=255)                                                                 gCTGGACATCGACCAGCCGCAGTTAATCGTCAAGGATACGCGTCTGGCGTTTGGTGAACTGGCTGCATGGGTTCGCCAGc              >  2:19328/1‑80 (MQ=255)                                                                 gCTGGACATCGACCAGCCGCAGTTAATCGTCAAGGATACGCGTCTGGCGTTTGGTGAACTGGCTGCATGGGTTCGCCAGc              >  1:19331/1‑80 (MQ=255)                                                                  cTGGACATCGACCAGCCGCAGTTAATCGTCAAGGATACGCGTCTGGCGTTTGGTGAACTGGCTGCATGGGTTCGCCAGc              >  2:19350/1‑79 (MQ=255)                                                                  cTGGACATCGACCAGCCGCAGTTAATCGTCAAGGATACGCGTCTGGCGTTTGGTGAACTGGCTGCATGGGTTCGCCAGc              >  1:19329/1‑79 (MQ=255)                                                                     gACATCGACCAGCCGCAGTTAATCGTCAAGGATACGCGTCTGGCGTTTGGTGAACTGGCTGCATGGGTTCGCCAGCAAg           <  1:19276/79‑1 (MQ=255)                                                                     gACATCGACCAGCCGCAGTTAATCGTCAAGGATACGCGTCTGGCGTTTGGTGAACTGGCTGCATGGGTTCGCCAGCAAGtt         <  1:19277/81‑1 (MQ=255)                                                                      aCATCGACCAGCCGCAGTTAATCGTCAAGGATACGCGTCTGGCGTTTGGTGAACTGGCTGCATGGGTTCGCCAGCAAGTTc        <  1:19278/81‑1 (MQ=255)                                                                        aTCGACCAGCCGCAGTTAATCGTCAAGGATACGCGTCTGGCGTTTGGTGAACTGGCTGCATGGGTTCGCCAGCAAGTTCCg      >  2:19338/1‑81 (MQ=255)                                                                        aTCGACCAGCCGCAGTTAATCGTCAAGGATACGCGTCTGGCGTTTGGTGAACTGGCTGCATGGGTTCGCCAGCAAGTTCCg      <  2:19279/81‑1 (MQ=255)                                                                        aTCGACCAGCCGCAGTTAATCGTCAAGGATACGCGTCTGGCGTTTGGTGAACTGGCTGCATGGGTTCGCCAGCAAGTTCCg      >  2:19351/1‑81 (MQ=255)                                                                           gACCAGCCGCAGTTAATCGTCAAGGATACGCGTCTGGCGTTTGGTGAACTGGCTGCATGGGTTCGCCAGCAAGTTCCGgcg   <  2:19280/81‑1 (MQ=255)                                                                           gACCAGCCGCAGTTAATCGTCAAGGATACGCGTCTGGCGTTTGGTGAACTGGCTGCATGGGTTCGCCAGCAAGTTCCGgc    <  1:19281/80‑1 (MQ=255)                                                                           gACCAGCCGCAGTTAATCGTCAAGGATACGCGTCTGGCGTTTGGTGAACTGGCTGCATGGGTTCGCCAGCAAGTTCCGgc    <  1:19283/80‑1 (MQ=255)                                                                           gACCAGCCGCAGTTAATCGTCAAGGATACGCGTCTGGCGTTTGGTGAACTGGCTGCATGGGTTCGCCAGCAAGTTCCGgc    <  1:19282/80‑1 (MQ=255)                                                                            aCCAGCCGCAGTTAATCGTCAAGGATACGCGTCTGGCGTTTGGTGAACTGGCTGCATGGGTTCGCCAGCAAGTTCCGgcgc  <  1:19284/81‑1 (MQ=255)                                                                               |                                                                              ATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTACTGGTTAGCCGTCCGCTGGACATCGACCTGCCGCAGTTAATCGTCAAGGATACGCGTCTGGCGTTTGGTGAACTGGCTGCATGGGTTCGCCAGCAAGTTCCGGCGC  >  NC_000913/94795‑94950

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑