Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,411,109	A→T	D280E (GAT→GAA)	ttcA ←	tRNA cytosine(32) 2‑sulfurtransferase TtcA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,411,109	0	A	T	100.0%	110.6 / NA	33	D280E (GAT→GAA)	ttcA	tRNA cytosine(32) 2‑sulfurtransferase TtcA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (21/12);  total (21/12)

GCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGTGGGTGATGCCTTTGAAATCGAACAGGTTGGTAT  >  NC_000913/1411036‑1411182                                                                          |                                                                          gCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGTTCAAACg                                                                    >  1:250527/1‑81 (MQ=255)     tAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGTTCAAACGc                                                                   >  2:250510/1‑78 (MQ=255)     tAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGTTCAAACGCCAg                                                                <  1:250470/81‑1 (MQ=255)       aCTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGTTCAAACGc                                                                   >  2:250535/1‑76 (MQ=255)        cTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGTTCAAACGCCAGAt                                                              >  1:250503/1‑80 (MQ=255)          cATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGTTCAAACGCCAGATCAc                                                           >  2:250531/1‑81 (MQ=255)             ccAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGTTCAAACGCCAGATCAcccc                                                        >  2:250505/1‑81 (MQ=255)              cAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGTTCAAACGCCAGATCAccccc                                                       <  1:250471/81‑1 (MQ=255)                 cTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGTTCAAACGCCAGATCACCCCCGtt                                                    <  1:250472/81‑1 (MQ=255)                    aTTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGTTCAAACGCCAGATCACCCCCGTTAAc                                                 <  2:250473/81‑1 (MQ=255)                         cATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGTTCAAACGCCAGATCACCCCCGTTAACCACt                                             <  1:250474/80‑1 (MQ=255)                            cttcttctGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGTTCAAACGCCAGATCACCCCCGTTAACCACtt                                            >  2:250508/1‑78 (MQ=255)                               cttctGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGTTCAAACGCCAGATCACCCCCGTTAACCACTTCAGa                                        <  1:250475/79‑1 (MQ=255)                                 tctGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGTTCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCg                                    >  1:250513/1‑81 (MQ=255)                                  ctGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGTTCAAACGCCAGATCACCCCCGTTAACCACTTCAGAAc                                      <  1:250476/78‑1 (MQ=255)                                  ctGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGTTCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGt                                   >  2:250507/1‑81 (MQ=255)                                   tGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGTTCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGTg                                  >  1:250532/1‑81 (MQ=255)                                    ggCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGTTCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGTgg                                 >  2:250518/1‑81 (MQ=255)                                     gCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGTTCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGTggg                                >  2:250515/1‑81 (MQ=255)                                     gCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGTTCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGTgg                                 <  1:250477/80‑1 (MQ=255)                                           aGCACGCCGGTTGTAGTGGGATCTCTTCGCGTTCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGTGGGTGATGc                          >  1:452271/1‑81 (MQ=255)                                             cACGCCGGTTGTAGTGGGATCTCTTCGCGTTCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGTGGGTGATGCCt                        >  1:250529/1‑81 (MQ=255)                                               cGCCGGTTGTAGTGGGATCTCTTCGCGTTCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGTGGGTGATGCCttt                      <  1:250478/81‑1 (MQ=255)                                                   ggTTGTAGTGGGATCTCTTCGCGTTCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGTGGGTGATGCCTTTGaaa                  >  1:250525/1‑81 (MQ=255)                                                       gTAGTGGGATCTCTTCGCGTTCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGTGGGTGATGCCTTTGAAATCg               >  2:250516/1‑80 (MQ=255)                                                           tGGGATCTCTTCGCGTTCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGTGGGTGATGCCTTTGAAATCGAACAg          <  1:250479/81‑1 (MQ=255)                                                             ggATCTCTTCGCGTTCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGTGGGTGATGCCTTTGAAATCGAACAGGt        <  1:250480/81‑1 (MQ=255)                                                             ggATCTCTTCGCGTTCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGTGGGTGATGCCTTTGAAATCGAACAGGt        >  2:250538/1‑81 (MQ=255)                                                              gATCTCTTCGCGTTCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGTGGGTGATGCCTTTGAAATCGAACAGGtt       <  2:250481/81‑1 (MQ=255)                                                              gATCTCTTCGCGTTCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGTGGGTGATGCCTTTGAAATCGAACAGGtt       >  1:250517/1‑81 (MQ=255)                                                                 ctctTCGCGTTCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGTGGGTGATGCCTTTGAAATCGAACAGGTTgg     >  1:250514/1‑80 (MQ=255)                                                                   ctTCGCGTTCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGTGGGTGATGCCTTTGAAATCGAACAGGTTGGTAt  >  2:250523/1‑81 (MQ=255)                                                                   ctTCGCGTTCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGTGGGTGATGCCTTTGAAATCGAACAGGTTGGTAt  >  2:250528/1‑81 (MQ=255)                                                                          |                                                                          GCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGTGGGTGATGCCTTTGAAATCGAACAGGTTGGTAT  >  NC_000913/1411036‑1411182

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑