Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,424,387	T→C	C202C (TGT→TGC)	trkG →	Rac prophage; K(+) transporter TrkG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,424,387	0	T	C	100.0%	72.3 / NA	21	C202C (TGT→TGC)	trkG	Rac prophage; K(+) transporter TrkG
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (12/9);  total (12/9)

CTCCCCGCCTGGCCGATACGTCACGGACACTGTGGATAACTTATTCTTTATTAGGTATTGCTTGTATTGTCTGTTATAGACTTGCAGGAATGCCTTTGTTTGATGCTATTTGTCACGGGATTTCCACAGTTTCGCTTGGTGGTTTCTC  >  NC_000913/1424314‑1424461                                                                          |                                                                           ctcCCCGCCTGGCCGATACGTCACGGACACTGTGGATAACTTATTCTTTATTAGGTATTGCTTGTATTGTCTGCTATAGAc                                                                     >  2:252288/1‑81 (MQ=255)                aTACGTCACGGACACTGTGGATAACTTATTCTTTATTAGGTATTGCTTGTATTGTCTGCTATAGACTTGCAGGAATGCCtt                                                      >  2:252291/1‑81 (MQ=255)                aTACGTCACGGACACTGTGGATAACTTATTCTTTATTAGGTATTGCTTGTATTGTCTGCTATAGACTTGCAGGAATGCCtt                                                      >  2:252286/1‑81 (MQ=255)                aTACGTCACGGACACTGTGGATAACTTATTCTTTATTAGGTATTGCTTGTATTGTCTGCTATAGACTTGCAGGAATGCCtt                                                      <  1:252268/81‑1 (MQ=255)                           acacTGTGGATAACTTATTCTTTATTAGGTATTGCTTGTATTGTCTGCTATAGACTTGCAGGAATGCCTTTGTTTGATGc                                            >  1:252301/1‑80 (MQ=255)                           acacTGTGGATAACTTATTCTTTATTAGGTATTGCTTGTATTGTCTGCTATAGACTTGCAGGAATGCCTTTGTTTGATGCt                                           <  2:252269/81‑1 (MQ=255)                             acTGTGGATAACTTATTCTTTATTAGGTATTGCTTGTATTGTCTGCTATAGACTTGCAGGAATGCCTTTGTTTGATGCTAt                                         <  1:252270/81‑1 (MQ=255)                                   gATAACTTATTCTTTATTAGGTATTGCTTGTATTGTCTGCTATAGACTTGCAGGAATGCCTTTGTTTGATGCTATTTGTCa                                   >  1:252289/1‑81 (MQ=255)                                           aTTCTTTATTAGGTATTGCTTGTATTGTCTGCTATAGACTTGCAGGAATGCCTTTGTTTGATGCTATTTGTCACGGGAt                             <  2:252271/79‑1 (MQ=255)                                             tCTTTATTAGGTATTGCTTGTATTGTCTGCTATAGACTTGCAGGAATGCCTTTGTTTGATGCTATTTGTCACGGGATTTc                          >  2:252290/1‑80 (MQ=255)                                              cTTTATTAGGTATTGCTTGTATTGTCTGCTATAGACTTGCAGGAATGCCTTTGTTTGATGCTATTTGTCACGGGATTTCca                        >  2:252287/1‑81 (MQ=255)                                                  attaGGTATTGCTTGTATTGTCTGCTATAGACTTGCAGGAATGCCTTTGTTTGATGCTATTTGTCACGGGATTTCCACAg                     <  2:252272/80‑1 (MQ=255)                                                  attaGGTATTGCTTGTATTGTCTGCTATAGACTTGCAGGAATGCCTTTGTTTGATGCTATTTGTCACGGGATTTCCACAg                     <  1:252273/80‑1 (MQ=255)                                                     aGGTATTGCTTGTATTGTCTGCTATAGACTTGCAGGAATGCCTTTGTTTGATGCTATTTGTCACGGGATTTCCACAGTTTc                 >  1:252297/1‑81 (MQ=255)                                                      ggTATTGCTTGTATTGTCTGCTATAGACTTGCAGGAATGCCTTTGTTTGATGCTATTTGTCACGGGATTTCCACAGTTTc                 <  2:252275/80‑1 (MQ=255)                                                      ggTATTGCTTGTATTGTCTGCTATAGACTTGCAGGAATGCCTTTGTTTGATGCTATTTGTCACGGGATTTCCACAGTTTc                 <  1:252274/80‑1 (MQ=255)                                                        tATTGCTTGTATTGTCTGCTATAGACTTGCAGGAATGCCTTTGTTTGATGCTATTTGTCACGGGATTTCCACAGTTTCGCt              >  2:252292/1‑81 (MQ=255)                                                         aTTGCTTGTATTGTCTGCTATAGACTTGCAGGAATGCCTTTGTTTGATGCTATTTGTCACGGGATTTCCACAGTTTCGCtt             >  2:252303/1‑81 (MQ=255)                                                              ttGTATTGTCTGCTATAGACTTGCAGGAATGCCTTTGTTTGATGCTATTTGTCACGGGATTTCCACAGTTTCGCTtggtgg        >  1:252294/1‑81 (MQ=255)                                                                   ttGTCTGCTATAGACTTGCAGGAATGCCTTTGTTTGATGCTATTTGTCACGGGATTTCCACAGTTTCGCTTGGTGGTTtc    <  2:252276/80‑1 (MQ=255)                                                                     gTCTGCTATAGACTTGCAGGAATGCCTTTGTTTGATGCTATTTGTCACGGGATTTCCACAGTTTCGCTTGGTGGTTtctc  >  1:252300/1‑80 (MQ=255)                                                                          |                                                                           CTCCCCGCCTGGCCGATACGTCACGGACACTGTGGATAACTTATTCTTTATTAGGTATTGCTTGTATTGTCTGTTATAGACTTGCAGGAATGCCTTTGTTTGATGCTATTTGTCACGGGATTTCCACAGTTTCGCTTGGTGGTTTCTC  >  NC_000913/1424314‑1424461

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑