Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,433,224	C→T	V151M (GTG→ATG)	pinR ←	Rac prophage; putative site‑specific recombinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,433,224	0	C	T	100.0%	47.5 / NA	16	V151M (GTG→ATG)	pinR	Rac prophage; putative site‑specific recombinase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (3/13);  total (3/13)

CACTTATACTTACACCTGACTTAATTCGTTCGAATACCACCTGTTTCTGTTCTTCATTTAACACAGGAGGTCGACCAAAACGTTTCCCTGCGCCGCGGGCTCTTACTATCCCGGAATGAGTGCGTTCAAGTAAAA  >  NC_000913/1433161‑1433295                                                                |                                                                        cACTTATACTTACACCTGACTTAATTCGTTCGAATACCACCTGTTTCTGTTCTTCATTTAACATAGGAGGTCGACCAAAAc                                                        <  2:253654/81‑1 (MQ=255)       taCTTACACCTGACTTAATTCGTTCGAATACCACCTGTTTCTGTTCTTCATTTAACATAGGAGGTCAACCAAAACGTTTc                                                   <  1:253655/80‑1 (MQ=255)         cTTACACCTGACTTAATTCGTTCGAATACCACCTGTTTCTGTTCTTCATTTAACATAGGAGGTCGACCAAAACGTTTCCCt                                                <  2:253656/81‑1 (MQ=255)                   aCTTAATTCGTTCGAATACCACCTGTTTCTGTTCTTCATTTAACATAGGAGGTCGACCAAAACGTTTCCCTGCGCCGCggg                                      <  1:253658/81‑1 (MQ=255)                   aCTTAATTCGTTCGAATACCACCTGTTTCTGTTCTTCATTTAACATAGGAGGTCGACCAAAACGTTTCCCTGCGCCGCgg                                       <  2:253657/80‑1 (MQ=255)                       aaTTCGTTCGAATACCACCTGTTTCTGTTCTTCATTTAACATAGGAGGTCGACCAAAACGTTTCCCTGCGCCGCGGGct                                    <  1:253659/79‑1 (MQ=255)                                gAATACCACCTGTTTCTGTTCTTCATTTAACATAGGAGGTCGACCAAAACGTTTCCCTGCGCCGCGGGCTCTTACTATccc                         <  1:253663/81‑1 (MQ=255)                                gAATACCACCTGTTTCTGTTCTTCATTTAACATAGGAGGTCGACCAAAACGTTTCCCTGCGCCGCGGGCTCTTACTATccc                         <  2:253660/81‑1 (MQ=255)                                gAATACCACCTGTTTCTGTTCTTCATTTAACATAGGAGGTCGACCAAAACGTTTCCCTGCGCCGCGGGCTCTTACTATccc                         <  2:253662/81‑1 (MQ=255)                                gAATACCACCTGTTTCTGTTCTTCATTTAACATAGGAGGTCGACCAAAACGTTTCCCTGCGCCGCGGGCTCTTACTATcc                          <  2:253661/80‑1 (MQ=255)                                      caccTGTTTCTGTTCTTCATTTAACATAGGAGGTCGACCAAAACGTTTCCCTGCGCCGCGGGCTCTTACTATCCCGGAAt                    >  2:253680/1‑80 (MQ=255)                                             ttCTGTTCTTCATTTAACATAGGAGGTCGACCAAAACGTTTCCCTGCGCCGCGGGCTCTTACTATCCCGGAATGAGTGCGt            >  2:253679/1‑81 (MQ=18)                                              tCTGTTCTTCATTTAACATAGGAGGTCGACCAAAACGTTTCCCTGCGCCGCGGGCTCTTACTATCCCGGAATGAGTGCGt            <  1:253664/80‑1 (MQ=21)                                                   tcttcATTTAACATAGGAGGTCGACCAAAACGTTTCCCTGCGCCGCGGGCTCTTACTATCCCGGAATGAGTGCGTTCAAg       >  2:253678/1‑80 (MQ=21)                                                    cttcATTTAACATAGGAGGTCGACCAAAACGTTTCCCTGCGCCGCGGGCTCTTACTATCCCGGAATGAGTGCGTTCAAGt      <  2:253665/80‑1 (MQ=21)                                                        aTTTAACATAGGAGGTCGACCAAAACGTTTCCCTGCGCCGCGGGCTCTTACTATCCCGGAATGAGTGCGTTCAAGTaaaa  <  2:253666/80‑1 (MQ=21)                                                                |                                                                        CACTTATACTTACACCTGACTTAATTCGTTCGAATACCACCTGTTTCTGTTCTTCATTTAACACAGGAGGTCGACCAAAACGTTTCCCTGCGCCGCGGGCTCTTACTATCCCGGAATGAGTGCGTTCAAGTAAAA  >  NC_000913/1433161‑1433295

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑