Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	113,776	A→G	E111E (GAA→GAG)	guaC →	GMP reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	113,776	0	A	G	100.0%	87.3 / NA	25	E111E (GAA→GAG)	guaC	GMP reductase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/10);  total (15/10)

GCGTTTATCAACAATTCTTCCGCTGATGTGCTGAAACATGTGATGGTTTCTACCGGTACGTCTGATGCGGATTTCGAAAAAACTAAACAGATTCTCGACCTGAACCCGGCATTAAACTTCGTTTGTATTGACGTGGCGAATGGTTATTCC  >  NC_000913/113699‑113848                                                                              |                                                                         gCGTTTATCAACAATTCTTCCGCTGATGTGCTGAAACATGTGATGGTTTCTACCGGTACGTCTGATGCGGATTTCGagaaa                                                                       <  2:23176/81‑1 (MQ=255)      tATCAACAATTCTTCCGCTGATGTGCTGAAACATGTGATGGTTTCTACCGGTACGTCTGATGCGGATTTCGAGAAAACTa                                                                   <  2:23177/80‑1 (MQ=255)        tCAACAATTCTTCCGCTGATGTGCTGAAACATGTGATGGTTTCTACCGGTACGTCTGATGCGGATTTCGAGAAAACTAAAc                                                                <  1:23178/81‑1 (MQ=255)              aTTCTTCCGCTGATGTGCTGAAACATGTGATGGTTTCTACCGGTACGTCTGATGCGGATTTCGAGAAAACTAAACAGATtc                                                          <  2:23179/81‑1 (MQ=255)               ttcttcCGCTGATGTGCTGAAACATGTGATGGTTTCTACCGGTACGTCTGATGCGGATTTCGAGAAAACTAAACAGATtct                                                         <  1:23180/81‑1 (MQ=255)                 cttcCGCTGATGTGCTGAAACATGTGATGGTTTCTACCGGTACGTCTGATGCGGATTTCGAGAAAACTAAACAGATtctc                                                        <  1:23181/80‑1 (MQ=255)                  ttcCGCTGATGTGCTGAAACATGTGATGGTTTCTACCGGTACGTCTGATGCGGATTTCGAGAAAACTAAACAGATTCTCGa                                                      >  1:23230/1‑81 (MQ=255)                       cTGATGTGCTGAAACATGTGATGGTTTCTACCGGTACGTCTGATGCGGATTTCGAGAAAACTAAACAGATTCTCGACCTg                                                  >  2:23204/1‑80 (MQ=255)                        tGATGTGCTGAAACATGTGATGGTTTCTACCGGTACGTCTGATGCGGATTTCGAGAAAACTAAACAGATTCTCGACCTGaa                                                >  1:23214/1‑81 (MQ=255)                         gATGTGCTGAAACATGTGATGGTTTCTACCGGTACGTCTGATGCGGATTTCGAGAAAACTAAACAGATTCTCGACCTGAAc                                               >  1:23218/1‑81 (MQ=255)                            gtgCTGAAACATGTGATGGTTTCTACCGGTACGTCTGATGCGGATTTCGAGAAAACTAAACAGATTCTCGACCTGAACCCg                                            >  1:23213/1‑81 (MQ=255)                              gCTGAAACATGTGATGGTTTCTACCGGTACGTCTGATGCGGATTTCGAGAAAACTAAACAGATTCTCGACCTGAACCCgg                                           >  2:23206/1‑80 (MQ=255)                               cTGAAACATGTGATGGTTTCTACCGGTACGTCTGATGCGGATTTCGAGAAAACTAAACAGATTCTCGACCTGAACCCGGc                                          <  1:23182/80‑1 (MQ=255)                                  aaaCATGTGATGGTTTCTACCGGTACGTCTGATGCGGATTTCGAGAAAACTAAACAGATTCTCGACCTGAACCCGGCAtt                                       <  1:23183/80‑1 (MQ=255)                                       tgtgATGGTTTCTACCGGTACGTCTGATGCGGATTTCGAGAAAACTAAACAGATTCTCGACCTGAACCCGGCATTAAACtt                                 <  1:23184/81‑1 (MQ=255)                                        gtgATGGTTTCTACCGGTACGTCTGATGCGGATTTCGAGAAAACTAAACAGATTCTCGACCTGAACCCGGCATTAAACTTc                                >  2:23219/1‑81 (MQ=255)                                           aTGGTTTCTACCGGTACGTCTGATGCGGATTTCGAGAAAACTAAACAGATTCTCGACCTGAACCCGGCATTAAACTTCGtt                             <  2:23185/81‑1 (MQ=255)                                             ggTTTCTACCGGTACGTCTGATGCGGATTTCGAGAAAACTAAACAGATTTTCGACCTGAACCCGGCATTAAACTTCGTTTg                           >  2:23224/1‑81 (MQ=255)                                                  cTACCGGTACGTCTGATGCGGATTTCGAGAAAACTAAACAGATTCTCGACCTGAACCCGGCATTAAACTTCGTTTGTAtt                       >  2:23199/1‑80 (MQ=255)                                                    aCCGGTACGTCTGACGCGGACTTCGAGAAAACTAAACAGATTCCCGACCTGAACCCGGCATTAAACTTCGTTTGTATTGAc                    >  2:23225/1‑81 (MQ=255)                                                     ccGGTACGTCTGATGCGGATTTCGAGAAAACTAAACAGATTCTCGACCTGAACCCGGCATTAAACTTCGTTTGTATTg                      <  1:23186/78‑1 (MQ=255)                                                          aCGTCTGATGCGGATTTCGAGAAAACTAAACAGATTCTCGACCTGAACCCGGCATTAAACTTCGTTTGTATTGACGTGGc               >  1:23227/1‑80 (MQ=255)                                                            gTCTGATGCGGATTTCGAGAAAACTAAACAGATTCTCGACCTGAACCCGGCATTAAACTTCGTTTGTATTGACGTGGCGaa            >  1:23226/1‑81 (MQ=255)                                                                   gCGGATTTCGAGAAAACTAAACAGATTCTCGACCTGAACCCGGCATTAAACTTCGTTTGTATTGACGTGGCGAATGGTTAt     >  1:23221/1‑81 (MQ=255)                                                                       aTTTCGAGAAAACTAAACAGATTCTCGACCTGAACCCGGCATTAAACTTCGTTTGTATTGACGTGGCGAATGGTTATTcc  >  1:23210/1‑80 (MQ=255)                                                                       aTTTCGAGAAAACTAAACAGATTCTCGACCTGAACCCGGCATTAAACTTCGTTTGTATTGACGTGGCGAATGGTTATTcc  >  2:23229/1‑80 (MQ=255)                                                                              |                                                                         GCGTTTATCAACAATTCTTCCGCTGATGTGCTGAAACATGTGATGGTTTCTACCGGTACGTCTGATGCGGATTTCGAAAAAACTAAACAGATTCTCGACCTGAACCCGGCATTAAACTTCGTTTGTATTGACGTGGCGAATGGTTATTCC  >  NC_000913/113699‑113848

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑