Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,513,132	G→A	V106M (GTG→ATG)	ydcT →	putative ABC transporter ATP‑binding protein YdcT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,513,132	0	G	A	100.0%	100.5 / NA	29	V106M (GTG→ATG)	ydcT	putative ABC transporter ATP‑binding protein YdcT
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (17/12);  total (17/12)

GTCTTTCAGGACTACGCGCTATTTCCGCATATGTCGATTCTTGACAATGTCGCCTATGGGCTGATGGTCAAAGGCGTGAATAAAAAGCAGCGGCACGCAATGGCGCAAGAGGCGCTGGAGAAAGTGGCGTTGGGGTTTGTACATCAACGTA  >  NC_000913/1513057‑1513207                                                                            |                                                                            gTCTTTCAGGACTACGCGCTATTTCCGCATATGTCGATTCTTGACAATGTCGCCTATGGGCTGATGGTCAAAGGCATGaa                                                                         >  1:266310/1‑80 (MQ=255)  tCTTTCAGGACTACGCGCTATTTCCGCATATGTCGATTCTTGACAATGTCGCCTATGGGCTGATGGTCAAAGGCATGAAt                                                                        >  2:266311/1‑80 (MQ=255)      tCAGGACTACGCGCTATTTCCGCATATGTCGATTCTTGACAATGTCGCCTATGGGCTGATGGTCAAAGGCATGAATaaa                                                                     >  2:266309/1‑79 (MQ=255)      tCAGGACTACGCGCTATTTCCGCATATGTCGATTCTTGACAATGTCGCCTATGGGCTGATGGTCAAAGGCATGAATaa                                                                      <  2:266265/78‑1 (MQ=255)                gcgcTATTTCCGCATATGTCGATTCTTGACAATGTCGCCTATGGGCTGATGGTCAAAGGCATGAATAAAAAGCAGCGGCAc                                                         <  1:266267/81‑1 (MQ=255)                gcgcTATTTCCGCATATGTCGATTCTTGACAATGTCGCCTATGGGCTGATGGTCAAAGGCATGAATAAAAAGCAGCGGCAc                                                         <  1:266266/81‑1 (MQ=255)                gcgcTATTTCCGCATATGTCGATTCTTGACAATGTCGCCTATGGGCTGATGGTCAAAGGCATGAATAAAAAGCAGCGGCAc                                                         >  1:266301/1‑81 (MQ=255)                 cgcTATTTCCGCATATGTCGATTCTTGACAATGTCGCCTATGGGCTGATGGTCAAAGGCATGAATAAAAAGCAGCGGCACg                                                        <  1:266268/81‑1 (MQ=255)                     aTTTCCGCATATGTCGATTCTTGACAATGTCGCCTATGGGCTGATGGTCAAAGGCATGAATAAAAAGCAGCGGCACGCAAt                                                    >  2:266300/1‑81 (MQ=255)                          cGCATATGTCGATTCTTGACAATGTCGCCTATGGGCTGATGGTCAAAGGCATGAATAAAAAGCAGCGGCACGCAATGgcg                                                >  1:266299/1‑80 (MQ=255)                            cATATGTCGATTCTTGACAATGTCGCCTATGGGCTGATGGTCAAAGGCATGAATAAAAAGCAGCGGCACGCAATGGCGCaa                                             <  1:266269/81‑1 (MQ=255)                             atatGTCGATTCTTGACAATGTCGCCTATGGGCTGATGGTCAAAGGCATGAATAAAAAGCAGCGGCACGCAATGGCGCAag                                            <  2:266270/81‑1 (MQ=255)                                tGTCGATTCTTGACAATGTCGCCTATGGGCTGATGGTCAAAGGCATGAATAAAAAGCAGCGGCACGCAATGGCGCAAGAgg                                         >  1:266293/1‑81 (MQ=255)                                   cGATTCTTGACAATGTCGCCTATGGGCTGATGGTCAAAGGCATGAATAAAAAGCAGCGGCACGCAATGGCGCAAGAGgcgc                                      >  2:266298/1‑81 (MQ=255)                                    gATTCTTGACAATGTCGCCTATGGGCTGATGGTCAAAGGCATGAATAAAAAGCAGCGGCACGCAATGGCGCAAGAGGCGCt                                     >  1:266304/1‑81 (MQ=255)                                             cAATGTCGCCTATGGGCTGATGGTCAAAGGCATGAATAAAAAGCAGCGGCACGCAATGGCGCAAGAGGCGCTGGAGAAAGt                            <  1:266271/81‑1 (MQ=255)                                               aTGTCGCCTATGGGCTGATGGTCAAAGGCATGAATAAAAAGCAGCGGCACGCAATGGCGCAAGAGGCGCTGGAGAAAGTgg                          >  1:266307/1‑81 (MQ=255)                                               aTGTCGCCTATGGGCTGATGGTCAAAGGCATGAATAAAAAGCAGCGGCACGCAATGGCGCAAGAGGCGCTGGAGAAAGTgg                          >  2:266305/1‑81 (MQ=255)                                               aTGTCGCCTATGGGCTGATGGTCAAAGGCATGAATAAAAAGCAGCGGCACGCAATGGCGCAAGAGGCGCTGGAGAAAGTgg                          <  2:266272/81‑1 (MQ=255)                                                    gCCTATGGGCTGATGGTCAAAGGCATGAATAAAAAGCAGCGGCACGCAATGGCGCAAGAGGCGCTGGAGAAAGTGGCGtt                      <  1:266273/80‑1 (MQ=255)                                                        aTGGGCTGATGGTCAAAGGCATGAATAAAAAGCAGCGGCACGCAATGGCGCAAGAGGCGCTGGAGAAAGTGGCGTTGGGGt                 >  2:266325/1‑81 (MQ=255)                                                        aTGGGCTGATGGTCAAAGGCATGAATAAAAAGCAGCGGCACGCAATGGCGCAAGAGGCGCTGGAGAAAGTGGCGTTGGGGt                 >  1:266315/1‑81 (MQ=255)                                                         tGGGCTGATGGTCAAAGGCATGAATAAAAAGCAGCGGCACGCAATGGCGCAAGAGGCGCTGGAGAAAGTGGCGTTGGGGtt                <  1:266274/81‑1 (MQ=255)                                                          gggCTGATGGTCAAAGGCATGAATAAAAAGCAGCGGCACGCAATGGCGCAAGAGGCGCTGGAGAAAGTGGCGTTGGGGttt               >  1:266308/1‑81 (MQ=255)                                                                 tGGTCAAAGGCATGAATAAAAAGCAGCGGCACGCAATGGCGCAAGAGGCGCTGGAGAAAGTGGCGTTGGGGTTTGTACAt         >  2:266320/1‑80 (MQ=255)                                                                     cAAAGGCATGAATAAAAAGCAGCGGCACGCAATGGCGCAAGAGGCGCTGGAGAAAGTGGCGTTGGGGTTTGTACATCAACg    >  1:266322/1‑81 (MQ=255)                                                                     cAAAGGCATGAATAAAAAGCAGCGGCACGCAATGGCGCAAGAGGCGCTGGAGAAAGTGGCGTTGGGGTTTGTACATCAACg    >  2:266306/1‑81 (MQ=255)                                                                      aaaGGCATGAATAAAAAGCAGCGGCACGCAATGGCGCAAGAGGCGCTGGAGAAAGTGGCGTTGGGGTTTGTACATCAACg    <  2:266275/80‑1 (MQ=255)                                                                       aaGGCATGAATAAAAAGCAGCGGCACGCAATGGCGCAAGAGGCTCTGGAGAAAGTGGCGTTGGGGTTTGTACATCAACGTa  <  2:266276/81‑1 (MQ=255)                                                                            |                                                                            GTCTTTCAGGACTACGCGCTATTTCCGCATATGTCGATTCTTGACAATGTCGCCTATGGGCTGATGGTCAAAGGCGTGAATAAAAAGCAGCGGCACGCAATGGCGCAAGAGGCGCTGGAGAAAGTGGCGTTGGGGTTTGTACATCAACGTA  >  NC_000913/1513057‑1513207

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑