Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,527,350	G→A	intergenic (+198/‑540)	yncH → / → rhsE	DUF5445 domain‑containing protein YncH/RhsE protein in rhs element

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,527,350	0	G	A	100.0%	30.5 / NA	10	intergenic (+198/‑540)	yncH/rhsE	DUF5445 domain‑containing protein YncH/RhsE protein in rhs element
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (6/4);  total (6/4)

ATGTTGATTCTTAATATAACCTAATCAATAGATTATCAAGTGAGAGCTACACATTATTGGAGGGGCAGGGGAAAACACCATTTTGGGAACAATGCATCAGGTACTCTTACCACTGAAGCAAGATCATCCGGCAC  >  NC_000913/1527287‑1527420                                                                |                                                                       aTGTTGATTCTTAATATAACCTAATCAATAGATTATCAAGTGAGAGCTACACATTATTGGAGGAGCAGGGGAAAACACCAt                                                       >  2:268668/1‑81 (MQ=255)     tGATTCTTAATATAACCTAATCAATAGATTATCAAGTGAGAGCTACACATTATTGGAGGAGCAGGGGAAAACACCATTTTg                                                   >  1:268681/1‑81 (MQ=255)       aTTCTTAATATAACCTAATCAATAGATTATCAAGTGAGAGCTACACATTATTGGAGGAGCAGGGGAAAACACCATTTTgg                                                  <  2:268662/80‑1 (MQ=255)           ttAATATAACCTAATCAATAGATTATCAAGTGAGAGCTACACATTATTGGAGGAGCAGGGGAAAACACCATTTTGGGAAc                                              >  2:268683/1‑80 (MQ=255)                   aCCTAATCAATAGATTATCAAGTGAGAGCTACACATTATTGGAGGAGCAGGGGAAAACACCATTTTGGGAACAATGCATc                                      <  1:268663/80‑1 (MQ=255)                          cAATAGATTATCAAGTGAGAGCTACACATTATTGGAGGAGCAGGGGAAAACACCATTTTGGGAACAATGCATCAGGTActc                              >  2:268684/1‑81 (MQ=255)                            aTAGATTATCAAGTGAGAGCTACACATTATTGGAGGAGCAGGGGAAAACACCATTTTGGGAACAATGCATCAGGTActct                             >  1:268686/1‑80 (MQ=255)                               gATTATCAAGTGAGAGCTACACATTATTGGAGGAGCAGGGGAAAACACCA‑TTTGGGAACAATGCATCAGGTACTCTTAcc                         <  2:268664/80‑1 (MQ=255)                                      aaGTGAGAGCTACACATTATTGGAGGAGCAGGGGAAAACACCATTTTGGGAACAATGCATCAGGTACTCTTACCACTGAAg                  >  1:268682/1‑81 (MQ=255)                                                      ttattGGAGGAGCAGGGGAAAACACCATTTTGGGAACAATGCATCAGGTACTCTTACCACTGAAGCAAGATCATCCGGCAc  <  2:268665/81‑1 (MQ=255)                                                                |                                                                       ATGTTGATTCTTAATATAACCTAATCAATAGATTATCAAGTGAGAGCTACACATTATTGGAGGGGCAGGGGAAAACACCATTTTGGGAACAATGCATCAGGTACTCTTACCACTGAAGCAAGATCATCCGGCAC  >  NC_000913/1527287‑1527420

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑