Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,539,530	A→G	S1021P (TCC→CCC)	narZ ←	nitrate reductase Z subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,539,530	0	A	G	100.0%	96.5 / NA	29	S1021P (TCC→CCC)	narZ	nitrate reductase Z subunit alpha
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (8/21);  total (8/21)

ATAGAGCTGCTGGCGTCCCGACAGCGTGCGCCACGGAATTAACTCATGAACGTTGGTGTATCCCGCGTTATAGGAGACGTGATCGCTTTCCAGACCGGACCAGGTGGGGCTGGAGATAATTTTACGCGGCTGCGCCTGAATATCGCGAAA  >  NC_000913/1539456‑1539605                                                                           |                                                                            aTAGAGCTGCTGGCGTCCCGACAGCGTGCGCCACGGAATTAACTCATGAACGTTGGTGTATCCCGCGTTATAGGGGACGTg                                                                       <  2:270560/81‑1 (MQ=255) aTAGAGCTGCTGGCGTCCCGACAGCGTGCGCCACGGAATTAACTCATGAACGTTGGTGTATCCCGCGTTATAGGGGACGTg                                                                       <  2:270559/81‑1 (MQ=255) aTAGAGCTGCTGGCGTCCCGACAGCGTGCGCCACGGAATTAACTCATGAACGTTGGTGTATCCCGCGTTATAGGGGACGTg                                                                       <  2:270558/81‑1 (MQ=255)          ctgGCGTCCCGACAGCGTGCGCCACGGAATTAACTCATGAACGTTGGTGTATCCCGCGTTATAGGGGACGTGATCGCTTTc                                                              <  2:270561/81‑1 (MQ=255)             gCGTCCCGACAGCGTGCGCCACGGAATTAACTCATGAACGTTGGTGTATCCCGCGTTATAGGGGACGTGATCGCTTTCCa                                                            <  1:270562/80‑1 (MQ=255)                    gACAGCGTGCGCCACGGAATTAACTCATGAACGTTGGTGTATCCCGCGTTATAGGGGACGTGATCGCTTTCCAGACCGGAc                                                    <  2:270563/81‑1 (MQ=255)                     aCAGCGTGCGCCACGGAATTAACTCATGAACGTTGGTGTATCCCGCGTTATAGGGGACGTGATCGCTTTCCAGACCGGAcc                                                   <  2:270565/81‑1 (MQ=255)                     aCAGCGTGCGCCACGGAATTAACTCATGAACGTTGGTGTATCCCGCGTTATAGGGGACGTGATCGCTTTCCAGACCGGAcc                                                   <  2:270564/81‑1 (MQ=255)                     aCAGCGTGCGCCACGGAATTAACTCATGAACGTTGGTGTATCCCGCGTTATAGGGGACGTGATCGCTTTCCAGACCGGAc                                                    >  1:270613/1‑80 (MQ=255)                        gCGTGCGCCACGGAATTAACTCATGAACGTTGGTGTATCCCGCGTTATAGGGGACGTGATCGCTTTCCAGACCGGACCAgg                                                >  2:270618/1‑81 (MQ=255)                             cgccgcGGAATTAACTCATGAACGTTGGTGTATCCCGCGTTATAGGGGACGTGATCGCTTTCCAGACCGGGCCAGGTggg                                            <  2:270566/80‑1 (MQ=39)                              gcCACGGAATTAACTCATGAACGTTGGTGTATCCCGCGTTATAGGGGACGTGATCGCTTTCCAGACCGGACCAGGTGGGGc                                          >  2:270610/1‑81 (MQ=255)                                cACGGAATTAACTCATGAACGTTGGTGTATCCCGCGTTATAGGGGACGTGATCGCTTTCCAGACCGGACCAGGTGGGGCTg                                        <  2:270567/81‑1 (MQ=255)                                   ggAATTAACTCATGAACGTTGGTGTATCCCGCGTTATAGGGGACGTGATCGCTTTCCAGACCGGACCAGGTGGGGCTg                                        >  1:270617/1‑78 (MQ=255)                                     aaTTAACTCATGAACGTTGGTGTATCCCGCGTTATAGGGGACGTGATCGCTTTCCAGACCGGACCAGGTGGGGCTGgaga                                    >  2:270606/1‑80 (MQ=255)                                         aaCTCATGAACGTTGGTGTATCCCGCGTTATAGGGGACGTGATCGCTTTCCAGACCGGACCAGGTGGGGCTGGAGATAAtt                               >  2:270614/1‑81 (MQ=255)                                              aTGAACGTTGGTGTATCCCGCGTTATAGGGGACGTGATCGCTTTCCAGACCGGACCAGGTGGGGCTGGAGATAATTTTa                            <  1:270569/79‑1 (MQ=255)                                              aTGAACGTTGGTGTATCCCGCGTTATAGGGGACGTGATCGCTTTCCAGACCGGACCAGGTGGGGCTGGAGATAATTTTa                            <  2:270568/79‑1 (MQ=255)                                               tGAACGTTGGTGTATCCCGCGTTATAGGGGACGTGATCGCTTTCCAGACCGGACCAGGTGGGGCTGGAGATAATTTTAcgc                         >  1:270619/1‑81 (MQ=255)                                                      tGGTGTATCCCGCGTTATAGGGGACGTGATCGCTTTCCAGACCGGACCAGGTGGGGCTGGAGATAATTTTACGCGGCTgcg                  >  2:270605/1‑81 (MQ=255)                                                       ggTGTATCCCGCGTTATAGGGGACGTGATCGCTTTCCAGACCGGACCAGGTGGGGCTGGAGATAATTTTACGCGGCTgcg                  <  2:270570/80‑1 (MQ=255)                                                           tATCCCGCGTTATAGGGGACGTGATCGCTTTCCAGACCGGACCAGGTGGGGCTGGAGATAATTTTACGCGGCTGCGCCTg              <  2:270571/80‑1 (MQ=255)                                                             tCCCGCGTTATAGGGGACGTGATCGCTTTCCAGACCGGACCAGGTGGGGCTGGAGATAATTTTACGCGGCTGCGCCTGaa            <  1:270572/80‑1 (MQ=255)                                                               ccGCGTTATAGGGGACGTGATCGCTTTCCAGACCGGACCAGGTGGGGCTGGAGATAATTTTACGCGGCTGCGCCTGAatat         <  1:270574/81‑1 (MQ=255)                                                               ccGCGTTATAGGGGACGTGATCGCTTTCCAGACCGGACCAGGTGGGGCTGGAGATAATTTTACGCGGCTGCGCCTGAata          <  1:270573/80‑1 (MQ=255)                                                                  tgTTATAGGGGACGTGATCGCTTTCCAGACCGGACCAGGTGGGGCTGGAGATAATTTTACGCGGCTGCGCCTGAATATcgc      <  1:270575/80‑1 (MQ=255)                                                                  cgTTATAGGGGACGTGATCGCTTTCCAGACCGGACCAGGTGGGGCTGGAGATAATTTTACGCGGCTGCGCCTGAATATcgc      <  1:270576/81‑1 (MQ=255)                                                                    ttATAGGGGACGTGATCGCTTTCCAGACCGGACCAGGTGGGGCTGGAGATAATTTTACGCGGCTGCGCCTGAATATCGCGa    <  1:270577/81‑1 (MQ=255)                                                                       taGGGGACGTGATCGCTTTCCAGACCGGACCAGGTGGGGCTGGAGATAATTTTACGCGGCTGCGCCTGAATATCGCGaaa  <  1:270578/80‑1 (MQ=255)                                                                           |                                                                            ATAGAGCTGCTGGCGTCCCGACAGCGTGCGCCACGGAATTAACTCATGAACGTTGGTGTATCCCGCGTTATAGGAGACGTGATCGCTTTCCAGACCGGACCAGGTGGGGCTGGAGATAATTTTACGCGGCTGCGCCTGAATATCGCGAAA  >  NC_000913/1539456‑1539605

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑