Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,612,952	G→A	S100S (TCC→TCT)	glsB ←	glutaminase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,612,952	0	G	A	100.0%	119.9 / NA	34	S100S (TCC→TCT)	glsB	glutaminase 2
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (14/20);  total (14/20)

TCGCAGACCACCAGCGCACCGGCATTAATGAACGGATTACGCGGTATACCCTGCTCCATTTCCAGTTGCACTAAGGAATTGAACGGTGATCCAGACGGATCTTTGCCGACGCGTTGCCAGATTTCCTCTTCGGAGTAATGACGCATGGCGA  >  NC_000913/1612878‑1613028                                                                           |                                                                             tCGCAGACCACCAGCGCACCGGCATTAATGAACGGATTACGCGGTATACCCTGCTCCATTTCCAGTTGCACTAAAGAAtt                                                                         <  1:281567/80‑1 (MQ=255)    cAGACCACCAGCGCACCGGCATTAATGAACGGATTACGCGGTATACCCTGCTCCATTTCCAGTTGCACTAAAGAATTGAAc                                                                     <  2:281568/81‑1 (MQ=255)        ccaccaGCGCACCGGCATTAATGAACGGATTACGCGGTATACCCTGCTCCATTTCCAGTTGCACTAAAGAATTGAACGGTg                                                                 <  2:281569/81‑1 (MQ=255)         caccaGCGCACCGGCATTAATGAACGGATTACGCGGTATACCCTGCTCCATTTCCAGTTGCACTAAAGAATTGAACGGTGa                                                                <  1:281570/81‑1 (MQ=255)              gcgcACCGGCATTAATGAACGGATTACGCGGTATACCCTGCTCCATTTCCAGTTGCACTAAAGAATTGAACGGTGATCCAg                                                           <  1:281571/81‑1 (MQ=255)              gcgcACCGGCATTAATGAACGGATTACGCGGTATACCCTGCTCCATTTCCAGTTGCACTAAAGAATTGAACGGTGATCCAg                                                           <  1:281572/81‑1 (MQ=255)                gcACCGGCATTAATGAACGGATTACGCGGTATACCCTGCTCCATTTCCAGTTGCACTAAAGAATTGAACGGTGATCCAGAc                                                         >  1:281607/1‑81 (MQ=255)                  aCCGGCATTAATGAACGGATTACGCGGTATACCCTGCTCCATTTCCAGTTGCACTAAAGAATTGAACGGTGATCCAGACgg                                                       <  1:281574/81‑1 (MQ=255)                  aCCGGCATTAATGAACGGATTACGCGGTATACCCTGCTCCATTTCCAGTTGCACTAAAGAATTGAACGGTGATCCAGACgg                                                       <  1:281573/81‑1 (MQ=255)                   ccGGCATTAATGAACGGATTACGCGGTATACCCTGCTCCATTTCCAGTTGCACTAAAGAATTGAACGGTGATCCAGACGGa                                                      <  2:281575/81‑1 (MQ=255)                   ccGGCATTAATGAACGGATTACGCGGTATACCCTGCTCCATTTCCAGTTGCACTAAAGAATTGAACGGTGATCCAGACGGa                                                      >  2:281604/1‑81 (MQ=255)                    cGGCATTAATGAACGGATTACGCGGTATACCCTGCTCCATTTCCAGTTGCACTAAAGAATTGAACGGTGATCCAGACGGAt                                                     <  1:281576/81‑1 (MQ=255)                     ggCATTAATGAACGGATTACGCGGTATACCCTGCTCCATTTCCAGTTGCACTAAAGAATTGAACGGTGATCCAGACGGATc                                                    <  1:281577/81‑1 (MQ=255)                          tAATGAACGGATTACGCGGTATACCCTGCTCCATTTCCAGTTGCACTAAAGAATTGAACGGTGATCCAGACGGATCTTTGc                                               >  2:281621/1‑81 (MQ=255)                               aaCGGATTACGCGGTATACCCTGCTCCATTTCCAGTTGCACTAAAGAATTGAACGGTGATCCAGACGGATCTTTGCCGAcg                                          <  2:281578/81‑1 (MQ=255)                                 cGGATTACGCGGTATACCCTGCTCCATTTCCAGTTGCACTAAAGAATTGAACGGTGATCCAGACGGATCTTTGCCGAcgcg                                        >  2:281619/1‑81 (MQ=255)                                   gATTACGCGGTATACCCTGCTCCATTTCCAGTTGCACTAAAGAATTGAACGGTGATCCAGACGGATCTTTGCCGACGCGtt                                      <  1:281579/81‑1 (MQ=255)                                         gcgGTATACCCTGCTCCATTTCCAGTTGCACTAAAGAATTGAACGGTGATCCAGACGGATCTTTGCCGACGCGTTGCCAGa                                <  2:281580/81‑1 (MQ=255)                                               taCCCTGCTCCATTTCCAGTTGCACTAAAGAATTGAACGGTGATCCAGACGGATCTTTGCCGACGCGTTGCCAGATTTcc                           <  1:281581/80‑1 (MQ=255)                                               taCCCTGCTCCATTTCCAGTTGCACTAAAGAATTGAACGGTGATCCAGACGGATCTTTGCCGACGCGTTGCCAGATTTCct                          >  2:281614/1‑81 (MQ=255)                                                aCCCTGCTCCATTTCCAGTTGCACTAAAGAATTGAACGGTGATCCAGACGGATCTTTGCCGACGCGTTGCCAGATTTCctc                         >  2:281628/1‑81 (MQ=255)                                                 cccTGCTCCATTTCCAGTTGCACTAAAGAATTGAACGGTGATCCAGACGGATCTTTGCCGACGCGTTGCCAGATTTCctct                        <  1:281582/81‑1 (MQ=255)                                                   cTGCTCCATTTCCAGTTGCACTAAAGAATTGAACGGTGATCCAGACGGATCTTTGCCGACGCGTTGCCAGATTTCCTCTTc                      <  2:281583/81‑1 (MQ=255)                                                    tGCTCCATTTCCAGTTGCACTAAAGAATTGAACGGTGATCCAGACGGATCTTTGCCGACGCGTTGCCAGATTTCCTCTTCg                     >  2:281615/1‑81 (MQ=255)                                                     gCTCCATTTCCAGTTGCACTAAAGAATTGAACGGTGATCCAGACGGATCTTTGCCGACGCGTTGCCAGATTTCCTCTTCg                     >  2:574504/1‑80 (MQ=255)                                                      cTCCATTTCCAGTTGCACTAAAGAATTGAACGGTGATCCAGACGGATCTTTGCCGACGCGTTGCCAGATTTCCTCTTCg                     >  1:281624/1‑79 (MQ=255)                                                        ccATTTCCAGTTGCACTAAAGAATTGAACGGTGATCCAGACGGATCTTTGCCGACGCGTTGCCAGATTTCCTCTTCGGAg                  >  1:281631/1‑80 (MQ=255)                                                         cATTTCCAGTTGCACTAAAGAATTGAACGGTGATCCAGACGGATCTTTGCCGACGCGTTGCCAGATTTCCTCTTCGGAg                  <  1:281585/79‑1 (MQ=255)                                                         cATTTCCAGTTGCACTAAAGAATTGAACGGTGATCCAGACGGATCTTTGCCGACGCGTTGCCAGATTTCCTCTTCGGAGt                 >  2:281618/1‑80 (MQ=255)                                                         cATTTCCAGTTGCACTAAAGAATTGAACGGTGATCCAGACGGATCTTTGCCGACGCGTTGCCAGATTTCCTCTTCGGAGt                 <  1:281584/80‑1 (MQ=255)                                                          aTTTCCAGTTGCACTAAAGAATTGAACGGTGATCCAGACGGATCTTTGCCGACGCGTTGCCAGATTTCCTCTTCGGAGTaa               >  1:281610/1‑81 (MQ=255)                                                             tCCAGTTGCACTAAAGAATTGAACGGTGATCCAGACGGATCTTTGCCGACGCGTTGCCAGATTTCCTCTTCGGAGTAATGa            >  2:281611/1‑81 (MQ=255)                                                               cAGTTGCACTAAAGAATTGAACGGTGATCCAGACGGATCTTTGCCGACGCGTTGCCAGATTTCCTCTTCGGAGTAATGACg          <  2:281586/81‑1 (MQ=255)                                                                        tAAAGAATTGAACGGTGATCCAGACGGATCTTTGCCGACGCGTTGCCAGATTTCCTCTTCGGAGTAATGACGCATGGCGa  >  2:281612/1‑80 (MQ=255)                                                                           |                                                                             TCGCAGACCACCAGCGCACCGGCATTAATGAACGGATTACGCGGTATACCCTGCTCCATTTCCAGTTGCACTAAGGAATTGAACGGTGATCCAGACGGATCTTTGCCGACGCGTTGCCAGATTTCCTCTTCGGAGTAATGACGCATGGCGA  >  NC_000913/1612878‑1613028

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑