Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,615,877	A→T	T39S (ACA→TCA)	yneK →	protein YneK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,615,877	0	A	T	100.0%	27.0 / NA	9	T39S (ACA→TCA)	yneK	protein YneK
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (3/6);  total (3/6)

GATGATTTTCCCGTACGTAATATTGCACCGCAGGTAAAAGAAGTTTTAAAGGATTTTATTGATGCACTTAGTACAATAATATGTAATGAAGAGTGGCGCACGTCTTTAAACATCAACTCAGCCACAAAAAAGATATTTAATAACC  >  NC_000913/1615805‑1615949                                                                         |                                                                         gatgatTTTCCCGTACGTAATATTGCACCGCAGGTAAAAGAAGTTTTAAAGGATTTTATTGATGCACTTAGTTCAataata                                                                  >  1:282032/1‑81 (MQ=255)              tacgtaATATTGCACCGCAGGTAAAAGAAGTTTTAAAGGATTTTATTGATGCACTTAGTTCAATAATATGTAATGAAGAGt                                                     <  2:282020/81‑1 (MQ=255)                     tatTGCACCGCAGGTAAAAGAAGTTTTAAAGGATTTTATTGATGCACTTAGTTCAATAATATGTAATGAAGAGTGGCGCAc                                              <  1:282021/81‑1 (MQ=255)                                   tAAAAGAAGTTTTAAAGGATTTTATTGATGCACTTAGTTCAATAATATGTAATGAAGAGTGGCGCACGTCTTTAAACATCa                                <  1:282022/81‑1 (MQ=255)                                    aaaaGAAGTTTTAAAGGATTTTATTGATGCACTTAGTTCAATAATATGTAATGAAGAGTGGCGCACGTCTTTAAACATCaa                               >  1:282039/1‑81 (MQ=255)                                              ttAAAGGATTTTATTGATGCACTTAGTTCAATAATATGTAATGAAGAGTGGCGCACGTCTTTAAACATCAACTCAGCcac                      <  1:282023/80‑1 (MQ=255)                                                   ggATTTTATTGATGCACTTAGTTCAATAATATGTAATGAAGAGTGGCGCACGTCTTTAAACATCAACTCAGCCACaaaa                  >  1:282037/1‑79 (MQ=255)                                                             gATGCACTTAGTTCAATAATATGTAATGAAGAGTGGCGCACGTCTTTAAACATCAACTCAGCCACAAAAAAGATATTtaa       <  1:282024/80‑1 (MQ=255)                                                                 cACTTAGTTCAATAATATGTAATGAAGAGTGGCGCACGTCTTTAAACATCAACTCAGCCACAAAAAAGATATTTAATAAcc  <  1:282025/81‑1 (MQ=255)                                                                         |                                                                         GATGATTTTCCCGTACGTAATATTGCACCGCAGGTAAAAGAAGTTTTAAAGGATTTTATTGATGCACTTAGTACAATAATATGTAATGAAGAGTGGCGCACGTCTTTAAACATCAACTCAGCCACAAAAAAGATATTTAATAACC  >  NC_000913/1615805‑1615949

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑