Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,671,696	T→C	intergenic (+12/‑81)	asr → / → ydgU	acid shock protein/uncharacterized protein YdgU

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,671,696	0	T	C	100.0%	47.1 / NA	15	intergenic (+12/‑81)	asr/ydgU	acid shock protein/uncharacterized protein YdgU
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (12/3);  total (12/3)

AGAAACACAGCCATCAGCAACCGGCAAAACCTGCTGCACAACCCGCAGCGTAAGTTTTCAACAGTAATGCTGGCGCGCCCCCTCGCGCCTGAAAATTACGGTGCTAAGCGGGTAACGTTTAGCACCGCCTTTAGCG  >  NC_000913/1671632‑1671767                                                                 |                                                                        aGAAACACAGCCATCAGCAACCGGCAAAACCTGCTGCACAACCCGCAGCGTAAGTTTTCAACAGCAATGCTGGCGCGcccc                                                         >  1:289471/1‑81 (MQ=255) aGAAACACAGCCATCAGCAACCGGCAAAACCTGCTGCACAACCCGCAGCGTAAGTTTTCAACAGCAATGCTGGCGCGcccc                                                         <  2:289451/81‑1 (MQ=255)          gCCATCAGCAACCGGCAAAACCTGCTGCACAACCCGCAGCGTAAGTTTTCAACAGCAATGCTGGCGCGCCCCCTCGCGCCt                                                <  1:289452/81‑1 (MQ=255)           ccATCAGCAACCGGCAAAACCTGCTGCACAACCCGCAGCGTAAGTTTTCAACAGCAATGCTGGCGCGCCCCCTCGCGCCTg                                               <  1:289453/81‑1 (MQ=255)                  cAACCGGCAAAACCTGCTGCACAACCCGCAGCGTAAGTTTTCAACAGCAATGCTGGCGCGCCCCCTCGCGCCTGAAAATTa                                        >  2:289470/1‑81 (MQ=255)                   aaCCGGCAAAACCTGCTGCACAACCCGCAGCGTAAGTTTTCAACAGCAATGCTGGCGCGCCCCCTCGCGCCTGAAAATTAc                                       >  1:289468/1‑81 (MQ=255)                   aaCCGGCAAAACCTGCTGCACAACCCGCAGCGTAAGTTTTCAACAGCAATGCTGGCGCGCCCCCTCGCGCCTGAAAATTAc                                       >  2:289469/1‑81 (MQ=255)                         cAAAACCTGCTGCACAACCCGCAGCGTAAGTTTTCAACAGCAATGCTGGCGCGCCCCCTCGCGCCTGAAAATTACGGTGc                                  >  1:289479/1‑80 (MQ=255)                           aaaCCTGCTGCACAACCCGCAGCGTAAGTTTTCAACAGCAATGCTGGCGCGCCCCCTCGCGCCTGAAAATTACGGTGCt                                 >  2:289467/1‑79 (MQ=255)                                            cGCAGCGTAAGTTTTCAACAGCAATGCTGGCGCGCCCCCTCGCGCCTGAAAATTACGGTGCTAAGCGGGTAACGTTTAGCa              >  1:289489/1‑81 (MQ=255)                                               aGCGTAAGTTTTCAACAGCAATGCTGGCGCGCCCCCTCGCGCCTGAAAATTACGGTGCTAAGCGGGTAACGTTTAGCACCg           >  2:289478/1‑81 (MQ=255)                                                  gTAAGTTTTCAACAGCAATGCTGGCGCGCCCCCTCGCGCCTGAAAATTACGGTGCTAAGCGGGTAACGTTTAGCACCGCCt        >  2:289472/1‑81 (MQ=255)                                                      gTTTTCAACAGCAATGCTGGCGCGCCCCCTCGCGCCTGAAAATTACGGTGCTAAGCGGGTAACGTTTAGCACCGCCTTTAg    >  2:289476/1‑81 (MQ=255)                                                       ttttCAACAGCAATGCTGGCGCGCCCCCTCGCGCCTGAAAATTACGGTGCTAAGCGGGTAACGTTTAGCACCGCCTTTAGc   >  2:289475/1‑81 (MQ=255)                                                        tttCAACAGCAATGCTGGCGCGCCCCCTCGCGCCTGAAAATTACGGTGCTAAGCGGGTAACGTTTAGCACCGCCTTTAGCg  >  2:289482/1‑81 (MQ=255)                                                                 |                                                                        AGAAACACAGCCATCAGCAACCGGCAAAACCTGCTGCACAACCCGCAGCGTAAGTTTTCAACAGTAATGCTGGCGCGCCCCCTCGCGCCTGAAAATTACGGTGCTAAGCGGGTAACGTTTAGCACCGCCTTTAGCG  >  NC_000913/1671632‑1671767

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑