Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,755,402	G→A	intergenic (‑261/‑296)	fumD ← / → pykF	fumarase D/pyruvate kinase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,755,402	0	G	A	100.0%	23.0 / NA	8	intergenic (‑261/‑296)	fumD/pykF	fumarase D/pyruvate kinase I
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (4/4);  total (4/4)

AAACAGTGAAGTTTTTGCGTAACCTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACT  >  NC_000913/1755331‑1755476                                                                        |                                                                           aaaCAGTGAAGTTTTTGCGTAACCTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAATTGTATAtt                                                                   >  1:302267/1‑81 (MQ=255)     aGTGAAGTTTTTGCGTAACCTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAATTGTATAtttttt                                                               <  2:302253/81‑1 (MQ=255)                  cGTAACCTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAATTGTATATTTTTTGAAACGCtgttt                                                   <  2:302254/80‑1 (MQ=255)                              ccTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAATTGTATATTTTTTGAAACGCTGTTTTTGTTTTCCttt                                       <  1:302255/80‑1 (MQ=255)                                                tGATAACAATTTATTGTCTAACAAATTGTATATTTTTTGAAACGCTGTTTTTGTTTTCCTTTTGGATTAATTTCAGCGta                     >  1:302265/1‑80 (MQ=255)                                                    aaCAATTTATTGTCTAACAAATTGTATATTTTTTGAAACGCTGTTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATg                >  1:302270/1‑81 (MQ=255)                                                             ttGTCTAACAAATTGTATATTTTTTGAAACGCTGTTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAAt       >  1:302276/1‑81 (MQ=255)                                                                    aCAAATTGTATATTTTTTGAAACGCTGTTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGAct  <  2:302256/79‑1 (MQ=255)                                                                        |                                                                           AAACAGTGAAGTTTTTGCGTAACCTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACT  >  NC_000913/1755331‑1755476

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑