Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,767,394	T→C	H431R (CAC→CGC)	ydiJ ←	putative FAD‑linked oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,767,394	0	T	C	100.0%	99.9 / NA	29	H431R (CAC→CGC)	ydiJ	putative FAD‑linked oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (16/13);  total (16/13)

GACGTGACCGAACATACCGTAGCTTAAGCCGTGGCTGTCGAGCAGCGCGCGAAATTCAGCAATATAATCCGCCAGGTGTTCCGGCGGTACGCAGGTATCCTCAGCAAACGGAATTGGCTTAGCGGCACCTTTGGCATTGCCAAGCAGACCAACG  >  NC_000913/1767318‑1767471                                                                             |                                                                              gACGTGACCGAACATACCGTAGCTTAAGCCGTGGCTGTCGAGCAGCGCGCGAAATTCAGCAATATAATCCGCCAGGCGTTc                                                                           >  1:304098/1‑81 (MQ=255)  aCGTGACCGAACATACCGTAGCTTAAGCCGTGGCTGTCGAGCAGCGCGCGAAATTCAGCAATATAATCCGCCAGGCGTTcc                                                                          <  1:304063/81‑1 (MQ=255)   cGTGACCGAACATACCGTAGCTTAAGCCGTGGCTGTCGAGCAGCGCGCGAAATTCAGCAATATAATCCGCCAGGCGTTCcg                                                                         >  2:304104/1‑81 (MQ=255)    gTGACCGAACATACCGTAGCTTAAGCCGTGGCTGTCGAGCAGCGCGCGAAATTCAGCAATATAATCCGCCAGGCGTTCcgg                                                                        >  2:304102/1‑81 (MQ=255)     tGACCGAACATACCGTAGCTTAAGCCGTGGCTGTCGAGCAGCGCGCGAAATTCAGCAATATAATCCGCCAGGCGTTCcggc                                                                       >  2:304107/1‑81 (MQ=255)        ccGAACATACCGTAGCTAAAGCCGTGGCTGTCGAGCAGCGCGCGAAATTCAGCAATATAATCCGCCAGGCGTTCcggcgg                                                                     >  2:304103/1‑80 (MQ=255)           aaCATACCGTAGCTTAAGCCGTGGCTGTCGAGCAGCGCGCGAAATTCAGCAATATAATCCGCCAGGCGTTCCGGCGGTACg                                                                 <  2:304064/81‑1 (MQ=255)             cATACCGTAGCTTAAGCCGTGGCTGTCGAGCAGCGCGCGAAATTCAGCAATATAATCCGCCAGGCGTTCCGGCGGTACGCa                                                               <  2:304065/81‑1 (MQ=255)                   gTAGCTTAAGCCGTGGCTGTCGAGCAGCGCGCGAAATTCAGCAATATAATCCGCCAGGCGTTCCGGCGGTACGCAGGTATc                                                         <  1:304067/81‑1 (MQ=255)                   gTAGCTTAAGCCGTGGCTGTCGAGCAGCGCGCGAAATTCAGCAATATAATCCGCCAGGCGTTCCGGCGGTACGCAGGTATc                                                         >  1:304105/1‑81 (MQ=255)                   gTAGCTTAAGCCGTGGCTGTCGAGCAGCGCGCGAAATTCAGCAATATAATCCGCCAGGCGTTCCGGCGGTACGCAGGTATc                                                         <  2:304066/81‑1 (MQ=255)                         tAAGCCGTGGCTGTCGAGCAGCGCGCGAAATTCAGCAATATAATCCGCCAGGCGTTCCGGCGGTACGCAGGTATCCTCAg                                                    >  1:304101/1‑80 (MQ=255)                          aaGCCGTGGCTGTCGAGCAGCGCGCGAAATTCAGCAATATAATCCGCCAGGCGTTCCGGCGGTACGCAGGTATCCTCAGc                                                   <  2:304068/80‑1 (MQ=255)                          aaGCCGTGGCTGTCGAGCAGCGCGCGAAAATCAGCAATATAATCCGCCAGGCGTTCCGGCGGTACGCAGGTATCCTCAg                                                    <  2:304069/79‑1 (MQ=255)                                     gTCGAGCAGCGCGCGAAATTCAGCAATATAATCCGCCAGGCGTTCCGGCGGTACGCAGGTATCCTCAGCAAACGGAATTgg                                       >  1:304108/1‑81 (MQ=255)                                       cGAGCAGCGCGCGAAATTCAGCAATATAATCCGCCAGGCGTTCCGGCGGTACGCAGGTATCCTCAGCAAACGGAATTGGCt                                     >  2:304113/1‑81 (MQ=255)                                         agcagcGCGCGAAATTCAGCAATATAATCCGCCAGGCGTTCCGGCGGTACGCAGGTATCCTCAGCAAACGGAATTGGCTTa                                   <  2:304070/81‑1 (MQ=255)                                            agcGCGCGAAATTCAGCAATATAATCCGCCAGGCGTTCCGGCGGTACGCAGGTATCCTCAGCAAACGGAATTGGCTTAGCg                                >  2:304117/1‑81 (MQ=255)                                               gcgcgAAATTCAGCAATATAATCCGCCAGGCGTTCCGGCGGTACGCAGGTATCCTCAGCAAACGGAATTGGCTTAGCGGCa                             >  2:304116/1‑81 (MQ=255)                                                cgcgAAATTCAGCAATATAATCCGCCAGGCGTTCCGGCGGTACGCAGGTATCCTCAGCAAACGGAATTGGCTTAGCGGCAc                            <  2:304072/81‑1 (MQ=255)                                                   gAAATTCAGCAATAGAATCCGCCAGGCGTTCCGGCGGTACGCAGGTATCCTCAGCAAACGGAATTGGCTTAGCGGCACCtt                         <  2:304073/81‑1 (MQ=255)                                                      aTTCAGCAATATAATCCGCCAGGCGTTCCGGCGGTACGCAGGTATCCTCAGCAAACGGAATTGGCTTAGCGGCACCTTTgg                      >  2:469697/1‑81 (MQ=255)                                                        tCAGCAATATAATCCGCCAGGCGTTCCGGCGGTACGCAGGTATCCTCAGCAAACGGAATTGGCTTAGCGGCACCTTTGGCa                    >  1:304092/1‑81 (MQ=255)                                                                   aTCCGCCAGGCGTTCCGGCGGTACGCAGGTATCCTCAGCAAACGGAATTGGCTTAGCGGCACCTTTGGCATTGCCAAGCAg         <  1:304074/81‑1 (MQ=255)                                                                      cGCCAGGCGTTCCGGCGGTACGCAGGTATCCTCAGCAAACGGAATTGGCTTAGCGGCACCTTTGGCATTGCCAAGCAGAc       <  1:304075/80‑1 (MQ=255)                                                                         cAGGCGTTCCGGCGGTACGCAGGTATCCTCAGCAAACGGAATTGGCTTAGCGGCACCTTTGGCATTGCCAAGCAGACCAAc   <  1:304076/81‑1 (MQ=255)                                                                         cAGGCGTTCCGGCGGTACGCAGGTATCCTCAGCAAACGGAATTGGCTTAGCGGCACCTTTGGCATTGCCAAGCAGACCAAc   >  2:304115/1‑81 (MQ=255)                                                                         cAGGCGTTCCGGCGGTACGCAGGTATCCTCAGCAAACGGAATTGGCTTAGCGGCACCTTTGGCATTGCCAAGCAGACCAAc   >  2:304120/1‑81 (MQ=255)                                                                          aGGCGTTCCGGCGGTACGCAGGTATCCTCAGCAAACGGAATTGGCTTAGCGGCACCTTTGGCATTGCCAAGCAGACCAACg  >  1:304123/1‑81 (MQ=255)                                                                             |                                                                              GACGTGACCGAACATACCGTAGCTTAAGCCGTGGCTGTCGAGCAGCGCGCGAAATTCAGCAATATAATCCGCCAGGTGTTCCGGCGGTACGCAGGTATCCTCAGCAAACGGAATTGGCTTAGCGGCACCTTTGGCATTGCCAAGCAGACCAACG  >  NC_000913/1767318‑1767471

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑