| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 206,823 | C→T | N566N (AAC→AAT) | dnaE → | DNA polymerase III subunit alpha |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 206,823 | 0 | C | T | 97.2% | 123.1 / NA | 36 | N566N (AAC→AAT) | dnaE | DNA polymerase III subunit alpha |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (17/18); minor base G (0/1); total (17/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GATAAAAGCGACGTTGAATACGCCGGACTGGTGAAGTTCGACTTCCTTGGTTTGCGTACGCTCACCATCATCAACTGGGCGCTGGAGATGATCAACAAGCGGCGGGCGAAGAATGGCGAGCCGCCGCTGGATATCGCTGCGATCCCGCTGGA > NC_000913/206749‑206900 | gATAAAAGCGACGTTGAATACGCCGGACTGGTGAAGTTCGACTTCCTTGGTTTGCGTACGCTCACCATCATCAATTGGgc < 1:41356/80‑1 (MQ=255)gATAAAAGCGACGTTGAATACGCCGGACTGGTGAAGTTCGACTTCCTTGGTTTGCGTACGCTCACCATCATCAATTGGgc < 2:41357/80‑1 (MQ=255) aTAAAAGCGACGTTGAATACGCCGGACTGGTGAAGTTCGACTTCCTTGGTTTGCGTACGCTCACCATCATCAATTGGgcgc > 2:498139/1‑81 (MQ=255) tAAAAGCGACGTTGAATACGCCGGACTGGTGAAGTTCGACTTCCTTGGTTTGCGTACGCTCACCATCATCAATTGGGCGCt < 2:41358/81‑1 (MQ=255) cGTTGAATACGCCGGACTGGTGAAGTTCGACTTCCTTGGTTTGCGTACGCTCACCATCATCAATTGGGCGCTGGAgatgat < 1:41359/81‑1 (MQ=255) gTTGAATACGCCGGACTGGTGAAGTTCGACTTCCTTGGTTTGCGTACGCTCACCATCATCAATTGGGCGCTGGAgatgat > 1:41402/1‑80 (MQ=255) tACGCCGGACTGGTGAAGTTCGACTTCCTTGATTTGCGTACGCTCACCATCATCAATTGGGCGCTGGAGATGATCAACAAg < 2:41360/81‑1 (MQ=255) ggACTGGTGAAGTTCGACTTCCTTGGTTTGCGTACGCTCACCATCATCAATTGGGCGCTGGAGATGATCAACAAGcggcgg > 2:41387/1‑81 (MQ=255) ggACTGGTGAAGTTCGACTTCCTTGGTTTGCGTACGCTCACCATCATCAATTGGGCGCTGGAGATGATCAACAAGcggcgg > 1:41392/1‑81 (MQ=255) ggTGAAGTTCGACTTCCTTGGTTTGCGTACGCTCACCATCATCAATTGGGCGCTGGAGATGATCAACAAGCggcgggcg > 2:41401/1‑79 (MQ=255) gTGAAGTTCGACTTCCTTGGTTTGCGTACGCTCACCATCATCAATTGGGCGCTGGAGATGATCAACAAGCGGCGGGCga < 1:41361/79‑1 (MQ=255) tGAAGTTCGACTTCCTTGGTTTGCGTACGCTCACCATCATCAATTGGGCGCTGGAGATGATCAACAAGCGGCGGGCgaaga < 1:41362/81‑1 (MQ=255) tGAAGTTCGACTTCCTTGGTTTGCGTACGCTCACCATCATCAATTGGGCGCTGGAGATGATCAACAAGCGGCGGGCgaag > 2:41400/1‑80 (MQ=255) gAAGTTCGACTTCCTTGGTTTGCGTACGCTCACCATCATCAATTGGGCGCTGGAGATGATCAACAAGCGGCGGGCgaagaa > 2:41404/1‑81 (MQ=255) aaGTTCGACTTCCTTGGTTTGCGTACGCTCACCATCATCAATTGGGCGCTGGAGATGATCAACAAGCGGCGGGCgaagaa < 2:41363/80‑1 (MQ=255) aaGTTCGACTTCCTTGGTTTGCGTACGCTCACCATCATCAATTGGGCGCTGGAGATGATCAACAAGCGGCGGGCGAAGAAt < 1:41364/81‑1 (MQ=255) tCGACTTCCTTGGTTTGCGTACGCTCACCATCATCAATTGGGCGCTGGAGATGATCAACAAGCGGCGGGCGAAGAATGGc > 1:41408/1‑80 (MQ=255) gACTTCCTTGGTTTGCGTACGCTCACCATCATCAATTGGGCGCTGGAGATGATCAACAAGCGGCGGGCGAAGAATGGCGa < 1:41365/80‑1 (MQ=255) gACTTCCTTGGTTTGCGTACGCTCACCATCATCAATTGGGCGCTGGAGATGATCAACAAGCGGCGGGCGAAGAATGGCGa < 1:41366/80‑1 (MQ=255) gACTTCCTTGGTTTGCGTACGCTCACCATCATCAATTGGGCGCTGGAGATGATCAACAAGCGGCGGGCGAAGAATGGCGAg > 2:41421/1‑81 (MQ=255) gACTTCCTTGGTTTGCGTACGCTCACCATCATCAATTGGGCGCTGGAGATGATCAACAAGCGGCGGGCGAAGAATGGCGAg > 1:41428/1‑81 (MQ=255) gACTTCCTTGGTTTGCGTACGCTCACCATCATCAATTGGGCGCTGGAGATGATCAACAAGCGGCGGGCGAAGAATGGCGAg < 2:41367/81‑1 (MQ=255) cTTCCTTGGTTTGCGTACGCTCACCATCATCAATTGGGCGCTGGAGATGATCAACAAGCGGCGGGCGAAGAATGGCGAgcc > 1:41417/1‑81 (MQ=255) cTTGGTTTGCGTACGCTCACCATCATCAATTGGGCGCTGGAGATGATCAACAAGCGGCGGGCGAAGAATGGCGAgccgcc < 2:41368/80‑1 (MQ=255) ggTTTGCGTACGCTCACCATCATCAATTGGGCGCTGGAGATGATCAACAAGCGGCGGGCGAAGAATGGCGAGCCGCCGCTg > 1:41405/1‑81 (MQ=255) tACGCTCACCATCATCAATTGGGCGCTGGAGATGATCAACAAGCGGCGGGCGAAGAATGGCGAGCCGCCGCTGGATATCGc < 2:41370/81‑1 (MQ=255) tACGCTCACCATCATCAATTGGGCGCTGGAGATGATCAACAAGCGGCGGGCGAAGAATGGCGAGCCGCCGCTGGATATCGc < 1:41369/81‑1 (MQ=255) gCTCACCATCATCAATTGGGCGCTGGAGATGATCAACAAGCGGCGGGCGAAGAATGGCGAGCCGCCGCTGGATATCGCTGc > 1:41420/1‑81 (MQ=255) gCTCACCATCATCAATTGGGCGCTGGAGATGATCAACAAGCGGCGGGCGAAGAATGGCGAGCCGCCGCTGGATATCGCTGc > 2:41413/1‑81 (MQ=255) gCTCACCATCATCAATTGGGCGCTGGAGATGATCAACAAGCGGCGGGCGAAGAATGGCGAGCCGCCGCTGGATATCGCTGc > 1:41410/1‑81 (MQ=255) atcaATTGGGCGCTGGAGATGATCAACAAGCGGCGGGCGAAGAATGGCGAGCCGCCGCTGGATATCGCTGCGATCCCGCt < 2:41371/80‑1 (MQ=255) atcaATTGGGCGCTGGAGATGATCAACAAGCGGCGGGCGAAGAATGGCGAGCCGCCGCTGGATATCGCTGCGATCCCGCTg < 2:41373/81‑1 (MQ=255) atcaATTGGGCGCTGGAGATGATCAACAAGCGGCGGGCGAAGAATGGCGAGCCGCCGCTGGATATCGCTGCGATCCCGCTg < 1:41372/81‑1 (MQ=255) atcaAGTGGGCGCTGGAGATGATCAACAAGCGGCGGGCGAAGAATGGCGAGGCGCCGCTGGATATCGCTGCGATcgcggt < 2:41374/80‑3 (MQ=255) caATTGGGCGCTGGAGATGATCAACAAGCGGCGGGCGAAGAATGGGGAGCCGCCGCTGGATATCGCTGCGATCCCGCTGga > 2:41448/1‑81 (MQ=255) caATTGGGCGCTGGAGATGATCAACAAGCGGCGGGCGAAGAATGGCGAGCCGCCGCTGGATATCGCTGCGATCCCGCTGga > 1:41412/1‑81 (MQ=255) | GATAAAAGCGACGTTGAATACGCCGGACTGGTGAAGTTCGACTTCCTTGGTTTGCGTACGCTCACCATCATCAACTGGGCGCTGGAGATGATCAACAAGCGGCGGGCGAAGAATGGCGAGCCGCCGCTGGATATCGCTGCGATCCCGCTGGA > NC_000913/206749‑206900 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |