Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,806,996	G→A	A209A (GCG→GCA)	pfkB →	6‑phosphofructokinase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,806,996	0	G	A	100.0%	108.1 / NA	31	A209A (GCG→GCA)	pfkB	6‑phosphofructokinase II
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (22/9);  total (22/9)

GTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGA  >  NC_000913/1806919‑1807070                                                                              |                                                                           gTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCACAg                                                                         <  2:309921/81‑1 (MQ=255)        cTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCACAGGAAATCg                                                                  >  2:309954/1‑81 (MQ=255)          aaCCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCACAGGAAATCGt                                                                 >  1:309957/1‑80 (MQ=255)            ccAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCACAGGAAATCGTTaa                                                              <  1:309922/81‑1 (MQ=255)                aaaGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCACAGGAAATCGTTAATAGc                                                          >  1:309949/1‑81 (MQ=255)                 aaGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCACAGGAAATCGTTAATAGCg                                                         <  1:309923/81‑1 (MQ=255)                     aCTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCACAGGAAATCGTTAATAGCGGc                                                       >  1:309945/1‑79 (MQ=255)                        cAGTTCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCACAGGAAATCGTTAATAGCGGCAAgcc                                                  >  2:309952/1‑79 (MQ=255)                           tGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCACAGGAAATCGTTAATAGCGGCAAGGCCaa                                               <  1:309924/81‑1 (MQ=255)                            gcgcTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCACAGGAAATCGTTAATAGCGGCAAGGCCaaa                                              >  2:309975/1‑81 (MQ=255)                              gcTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCACAGGAAATCGTTAATAGCGGCAAGGCCAAACg                                            >  2:309956/1‑81 (MQ=255)                              gcTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCACAGGAAATCGTTAATAGCGGCAAGGCCAAACg                                            >  2:309955/1‑81 (MQ=255)                               cTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCACAGGAAATCGTTAATAGCGGCAAGGCCAAACgg                                           >  2:309972/1‑81 (MQ=255)                                tGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCACAGGAAATCGTTAATAGCGGCAAGGCCAAACggg                                          >  2:309965/1‑81 (MQ=255)                                           gAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCACAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTcc                               >  1:309953/1‑81 (MQ=255)                                            aaCTCACCCAGCCGGACGATGTCCGCAAAGCCGCACAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTccc                              >  2:309960/1‑81 (MQ=255)                                                   ccAGCCGGACGATGTCCGCAAAGCCGCACAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTc                        >  1:309966/1‑80 (MQ=255)                                                     aGCCGGACGATGTCCGCAAAGCCGCACAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCca                      >  2:309963/1‑80 (MQ=255)                                                         ggACGATGTCCGCAAAGCCGCACAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAg                  >  1:309964/1‑80 (MQ=255)                                                           aCGATGTCCGCAAAGCCGCACAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAg               <  2:309925/81‑1 (MQ=255)                                                             gATGTCCGCAAAGCCGCACAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAgcg             >  1:309980/1‑81 (MQ=255)                                                              aTGTCCGCAAAGCCGCACAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAgcgc            >  1:309950/1‑81 (MQ=255)                                                               tGTCCGCAAAGCCGCACAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCt           <  2:309926/81‑1 (MQ=255)                                                                   cGCAAAGCCGCACAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGgt       <  2:309927/81‑1 (MQ=255)                                                                    gCAAAGCCGCACAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGgtg      >  2:309969/1‑81 (MQ=255)                                                                     cAAAGCCGCACAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGgtgt     >  2:309977/1‑81 (MQ=255)                                                                       aaGCCGCACAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGtt    >  2:309968/1‑80 (MQ=255)                                                                       aaGCCGCACAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGtt    >  2:309983/1‑80 (MQ=255)                                                                       aaGCCGCACAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTg   <  2:309928/81‑1 (MQ=255)                                                                        aGCCGCACAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGa  <  2:309929/81‑1 (MQ=255)                                                                          ccGCACAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGa  >  1:309973/1‑79 (MQ=255)                                                                              |                                                                           GTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGA  >  NC_000913/1806919‑1807070

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑