Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,810,695	T→C	M162T (ATG→ACG)	ydjM →	putative inner membrane protein regulated by LexA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,810,695	0	T	C	100.0%	42.6 / NA	13	M162T (ATG→ACG)	ydjM	putative inner membrane protein regulated by LexA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (5/8);  total (5/8)

CTGGTTCCCCAAAAGGGCAACCAACTGGAACGTTTTATCTGCATGGCATTATTTGTCTGGTCGGTATGGATGCCCCATTCATTACCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATACCTTGCAGATC  >  NC_000913/1810625‑1810762                                                                       |                                                                    cTGGTTCCCCAAAAGGGCAACCAACTGGAACGTTTTATCTGCATGGCATTATTTGTCTGGTCGGTATGGACGCCCcattca                                                           <  1:310457/81‑1 (MQ=255)             aaGGGCAACCAACTGGAACGTTTTATCTGCATGGCATTATTTGTCTGGTCGGTATGGACGCCCCATTCATTACCCGAGAAc                                               <  2:310458/81‑1 (MQ=255)                       aacTGGAACGTTTTATCTGCATGGCATTATTTGTCTGGTCGGTATGGACGCCCCATTCATTACCCGAGAACAGCGCTGTTc                                     <  1:310459/81‑1 (MQ=255)                          tGGAACGTTTTATCTGCATGGCATTATTTGTCTGGTCGGTATGGACGCCCCATTCATTACCCGAGAACAGCGCTGTTCGtt                                  >  1:310479/1‑81 (MQ=255)                          tGGAACGTTTTATCTGCATGGCATTATTTGTCTGGTCGGTATGGACGCCCCATTCATTACCCGAGAACAGCGCTGTTCGtt                                  >  2:310482/1‑81 (MQ=255)                            gAACGTTTTATCTGCATGGCATTATTTGTCTGGTCGGTATGGACGCCCCATTCATTACCCGAGAACAGCGCTGTTCGtt                                  <  2:310460/79‑1 (MQ=255)                              aCGTTTTATCTGCATGGCATTATTTGTCTGGTCGGTATGGACGCCCCATTCATTACCCGAGAACAGCGCTGTTCGTTGGTc                              <  1:310461/81‑1 (MQ=255)                                          cATGGCATTATTTGTCTGGTCGGTATGGACGCCCCATTCATTACCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATg                    <  2:310462/79‑1 (MQ=255)                                           aTGGCATTATTTGTCTGGTCGGTATGGACGCCCCATTCATTACCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGATc                 <  1:310463/81‑1 (MQ=255)                                                attattTGTCTGGTCGGTATGGACGCCCCATTCATTACCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATAc            >  1:310483/1‑81 (MQ=255)                                                    tttGTCTGGTCGGTATGGACGCCCCATTCATTACCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATACCtt         <  2:310464/80‑1 (MQ=255)                                                     ttGTCTGGTCGGTATGGACGCCCCATTCATTACCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATACCTTg        >  2:310484/1‑80 (MQ=255)                                                            gTCGGTATGGACGCCCCATTCATTACCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATACCTTGCAGATc  >  2:310485/1‑79 (MQ=255)                                                                       |                                                                    CTGGTTCCCCAAAAGGGCAACCAACTGGAACGTTTTATCTGCATGGCATTATTTGTCTGGTCGGTATGGATGCCCCATTCATTACCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATACCTTGCAGATC  >  NC_000913/1810625‑1810762

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑