Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,831,779	C→T	A68A (GCG→GCA)	astC ←	succinylornithine transaminase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,831,779	0	C	T	100.0%	86.2 / NA	26	A68A (GCG→GCA)	astC	succinylornithine transaminase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (14/12);  total (14/12)

TGGCGTCGATCAATTTTTTCGCCAGTCGCAGTACCGGCTCGTTGGTGTAACCGTTGCCGGTATGCCAGAACTTACTCGCCTGTTCGTTCAGCGCTTCACGCAGTTCCGGATGCGCATGGCCCAGCGCGTTCACCGCAATGCCACCCGCGAAG  >  NC_000913/1831703‑1831854                                                                             |                                                                            tGGCGTCGATCAATTTTTTCGCCAGTCGCAGTACCGGCTCGTTGGTGTAACCGTTGCCGGTATGCCAGAACTTACTTGCCt                                                                         <  1:313627/81‑1 (MQ=255)  ggCGTCGATCAATTTTTTCGCCAGTCGCAGTACCGGCTCGTTGGTGTAACCGTTGCCGGTATGCCAGAACTTACTTGCCTg                                                                        >  2:313661/1‑81 (MQ=255)  ggCGTCGATCAATTTTTTCGCCAGTCGCAGTACCGGCTCGTTGGTGTAACCGTTGCCGGTATGCCAGAACTTACTTGCCTg                                                                        >  1:313647/1‑81 (MQ=255)     gTCGATCAATTTTTTCGCCAGTCGCAGTACCGGCTCGTTGGTGTAACCGTTGCCGGTATGCCAGAACTTACTTGCCTgttc                                                                     >  1:313672/1‑81 (MQ=255)     gTCGATCAATTTTTTCGCCAGTCGCAGTACCGGCTCGTTGGTGTAACCGTTGCCGGTATGCCAGAACCTACTTGCCTgttc                                                                     >  2:313656/1‑81 (MQ=255)        gATCAATTTTTTCGCCAGTCGCAGTACCGGCTCGTTGGTGTAACCGTTGCCGGTATGCCAGAACTTACTTGCCTgttcgtt                                                                  <  1:313629/81‑1 (MQ=255)        gATCAATTTTTTCGCCAGTCGCAGTACCGGCTCGTTGGTGTAACCGTTGCCGGTATGCCAGAACTTACTTGCCTgttcgtt                                                                  >  2:313665/1‑81 (MQ=255)        gATCAATTTTTTCGCCAGTCGCAGTACCGGCTCGTTGGTGTAACCGTTGCCGGTATGCCAGAACTTACTTGCCTgttcgtt                                                                  <  2:313628/81‑1 (MQ=255)          tCAATTTTTTCGCCAGTCGCAGTACCGGCTCGTTGGTGTAACCGTTGCCGGTATGCCAGAACTTACTTGCCTGTTCGTTCa                                                                <  2:313630/81‑1 (MQ=255)                     gCCAGTCGCAGTACCGGCTCGTTGGTGTAACCGTTGCCGGTATGCCAGAACTTACTTGCCTGTTCGTTCAGCGCTTCACGc                                                     <  2:313632/81‑1 (MQ=255)                     gCCAGTCGCAGTACCGGCTCGTTGGTGTAACCGTTGCCGGTATGCCAGAACTTACTTGCCTGTTCGTTCAGCGCTTCACGc                                                     <  2:313631/81‑1 (MQ=255)                      ccAGTCGCAGTACCGGCTCGTTGGTGTAACCGTTGCCGGTATGCCAGAACTTACTTGCCTGTTCGTTCAGCGCTTCACGCa                                                    <  2:313633/81‑1 (MQ=255)                       cAGTCGCAGTACCGGCTCGTTGGTGTAACCGTTGCCGGTATGCCAGAACTTACTTGCCTGTTCGTTCAGCGCTTCACGCAg                                                   >  2:313659/1‑81 (MQ=255)                        aGTCGCAGTACCGGCTCGTTGGTGTAACCGTTGCCGGTATGCCAGAACTTACTTGCCTGTTCGTTCAGCGCTTCACGCAGt                                                  >  2:313660/1‑81 (MQ=255)                         gTCGCAGTACCGGCTCGTTGGTGTAACCGTTGCCGGTATGCCAGAACTTACTTGCCTGTTCGTTCAGCGCTTCACGCAGtt                                                 <  1:313634/81‑1 (MQ=255)                               gTACCGGCTCGTTGGTGTAACCGTTGCCGGTATGCCAGAACTTACTTGCCTGTTCGTTCAGCGCTTCACGCAGTTCCGGAt                                           >  2:313669/1‑81 (MQ=255)                                   cGGCTCGTTGGTGTAACCGTTGCCGGTATGCCAGAACTTACTTGCCTGTTCGTTCAGCGCTTCACGCAGTTCCGGATgcgc                                       <  2:313635/81‑1 (MQ=255)                                   cGGCTCGTTGGTGTAACCGTTGCCGGTATGCCAGAACTTACTTGCCTGTTCGTTCAGCGCTTCACGCAGTTCCGGATgcg                                        >  2:313666/1‑80 (MQ=255)                                        cGTTGGTGTAACCGTTGCCGGTATGCCAGAACTTACTTGCCTGTTCGTTCAGCGCTTCACGCAGTTCCGGATGCGCATGGc                                  >  1:313663/1‑81 (MQ=255)                                            ggTGTAACCGTTGCCGGTATGCCAGAACTTACTTGCCTGTTCGTTCAGCGCTTCACGCAGTTCCGGATGCGCATGGCCCAg                              >  2:313664/1‑81 (MQ=255)                                             gtgtAACCGTTGCCGGTATGCCAGAACTTACTTGCCTGTTCGTTCAGCGCTTCACGCAGTTCCGGATGCGCATGGCCCAgc                             <  2:313636/81‑1 (MQ=255)                                                              aTGCCAGAACTTACTTGCCTGTTCGTTCAGCGCTTCACGCAGTTCCGGATGCGCATGGCCCAGCGCGTTCACCGCAATGc             >  1:313675/1‑80 (MQ=255)                                                                gCCAGAACTTACTTGCCTGTTCGTTCAGCGCTTCACGCAGTTCCGGATGCGCATGGCCCAGCGCGTTCACCGCAATGCCAc          >  2:313668/1‑81 (MQ=255)                                                                  cAGAACTTACTTGCCTGTTCGTTCAGCGCTTCACGCAGTTCCGGATGCGCATGGCCCAGCGCGTTCACCGCAATGCCAccc        <  1:313637/81‑1 (MQ=255)                                                                     aaCTTACTTGCCTGTTCGTTCAGCGCTTCACGCAGTTCCGGATGCGCATGGCCCAGCGCGTTCACCGCAATGCCACCcgc      >  1:313670/1‑80 (MQ=255)                                                                          aCTTGCCTGTTCGTTCAGCGCTTCACGCAGTTCCGGATGCGCATGGCCCAGCGCGTTCACCGCAATGCCACCCGCGAAg  <  1:313638/79‑1 (MQ=255)                                                                             |                                                                            TGGCGTCGATCAATTTTTTCGCCAGTCGCAGTACCGGCTCGTTGGTGTAACCGTTGCCGGTATGCCAGAACTTACTCGCCTGTTCGTTCAGCGCTTCACGCAGTTCCGGATGCGCATGGCCCAGCGCGTTCACCGCAATGCCACCCGCGAAG  >  NC_000913/1831703‑1831854

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑