Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,875,852	G→A	L60L (TTG→TTA)	nimT →	2‑nitroimidazole exporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,875,852	0	G	A	100.0%	91.3 / NA	27	L60L (TTG→TTA)	nimT	2‑nitroimidazole exporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (15/12);  total (15/12)

ACCGTTACTGGATACGATTCGTTCCGCTTACTCGCTGACGACAGCGCAAACCGGCTTATTGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTAGCGCGACGTTTTGGTATGGAACGTAGCCTGTTTGCCGCGT  >  NC_000913/1875774‑1875928                                                                               |                                                                             aCCGTTACTGGATACGATTCGTTCCGCTTACTCGCTGACGACAGCGCAAACCGGCTTATTGACCACCCTGCCATTATTAGc                                                                            >  2:320433/1‑81 (MQ=255)           gATACGATTCGTTCCGCTTACTCGCTGACGACAGCGCAAACCGGCTTATTGACCACCCTGCCATTATTAGCCTTTGCGCTa                                                                  <  1:320406/81‑1 (MQ=255)               cGATTCGTTCCGCTTACTCGCTGACGACAGCGCAAACCGGCTTATTGACCACCCTGCCATTATTAGCCTTTGCGCTAAtc                                                               <  2:320407/80‑1 (MQ=255)                 aTTCGTTCCGCTTACTCGCTGACGACAGCGCAAACCGGCTTATTGACCACCCTGCCATTATTAGCCTTTGCGCTAATCTCa                                                            <  1:320408/81‑1 (MQ=255)                     gTTCCGCTTACTCGCTGACGACAGCGCAAACCGGCTTATTGACCACCCTGCCATTATTAGCCTTTGCGCTAATCTCACCtt                                                        >  2:320453/1‑81 (MQ=255)                              aCTCGCTGACGACAGCGCAAACCGGCTTATTGACCACCCTGCCATTATTAGCCTTTGCGCTAATCTCACCTTTGGCTGccc                                               >  1:320442/1‑81 (MQ=255)                                    tGACGACAGCGCAAACCGGCTTATTGACCACCCTGCCATTATTAGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTAg                                         >  2:320439/1‑81 (MQ=255)                                     gacgacAGCGCAAACCGGCTTATTGACCACCCTGCCATTATTAGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTAgc                                        >  1:320432/1‑81 (MQ=255)                                      atgacAGCGCAAACCGGCTTATTGACCACCCTGCCATTATTAGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTAgcg                                       >  2:320441/3‑81 (MQ=255)                                      acgacAGCGCAAACCGGCTTATTGACCACCCTGCCATTATTAGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTAgc                                        <  1:320409/80‑1 (MQ=255)                                      acgacAGCCCAAACCGGCTTATTGACCACCCTACCCTTATTAGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTAgcg                                       <  1:320410/81‑1 (MQ=255)                                       cgacAGCGCAAACCGGCTTATTGACCACCCTGCCATTATTAGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTAgcgc                                      <  2:320411/81‑1 (MQ=255)                                         acAGCGCAAACCGGCTTATTGACCACCCTGCCATTATTAGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTAGCGCGa                                    >  1:320434/1‑81 (MQ=255)                                                aaaCCGGCTTATTGACCACCCTGCCATTATTAGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTAGCGCGACGTTTTg                             <  2:320412/81‑1 (MQ=255)                                                       cTTATTGACCACCCTGCCATTATTAGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTAGCGCGACGTTTTGGTATgg                       >  1:320446/1‑80 (MQ=255)                                                          aTTGACCACCCTGCCATTATTAGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTAGCGCGACGTTTTGGTATGGAACg                   >  2:320445/1‑81 (MQ=255)                                                          aTTGACCACCCTGCCATTATTAGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTAGCGCGACGTTTTGGTATGGAACg                   >  2:320437/1‑81 (MQ=255)                                                               ccaccCTGCCATTATTAGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTAGCGCGACGTTTTGGTATGGAACGTAGcc              >  2:320436/1‑81 (MQ=255)                                                                caccCTGCCATTATTAGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTAGCGCGACGTTTTGGTATGGAACGTAGCCt             >  2:320435/1‑81 (MQ=255)                                                                  cccTGCCATTATTAGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTAGCGCGACGTTTTGGTATGGAACGTAGCCTGt           <  2:320413/81‑1 (MQ=255)                                                                  cccTGCCATTATTAGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTAGCGCGACGTTTTGGTATGGAACGTAGCCTGt           >  1:320449/1‑81 (MQ=255)                                                                    cTGCCATTATTAGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTAGCGCGACGTTTTGGTATGGAACGTAGCCTGttt         <  2:320416/81‑1 (MQ=255)                                                                    cTGCCATTATTAGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTAGCGCGACGTTTTGGTATGGAACGTAGCCTGttt         <  2:320415/81‑1 (MQ=255)                                                                    cTGCCATTATTAGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTAGCGCGACGTTTTGGTATGGAACGTAGCCTGttt         <  2:320414/81‑1 (MQ=255)                                                                        cATTATTAGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTAGCGCGACGTTTTGGTATGGAACGTAGCCTGTTTGCcg     <  1:320417/81‑1 (MQ=255)                                                                            attAGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTAGCGCGACGTTTTGGTATGGAACGTAGCCTGTTTGCCGCGt  >  2:416270/1‑80 (MQ=255)                                                                            attAGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTAGCGCGACGTCTTGGTATGGAACGTAGCCTGTTTGCCGCGt  >  2:320438/1‑80 (MQ=255)                                                                               |                                                                             ACCGTTACTGGATACGATTCGTTCCGCTTACTCGCTGACGACAGCGCAAACCGGCTTATTGACCACCCTGCCATTATTGGCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTAGCGCGACGTTTTGGTATGGAACGTAGCCTGTTTGCCGCGT  >  NC_000913/1875774‑1875928

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑