Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,900,559	C→A	E343* (GAA→TAA)	yoaE ←	putative inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,900,559	0	C	A	100.0%	96.6 / NA	28	E343* (GAA→TAA)	yoaE	putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (14/14);  total (14/14)

GAAGCAATCGCCGCCACATCAACGCCCTCTTCCAGCGCCACCAGCAGTTCTTTAGCACGTACAATACCGATGATTTCATCCAGTTCACCGCGACATACCGGGAACAGACTGTGCGGTGAAGAGAGCAGTTGCTCGCGGATTTCATCGACC  >  NC_000913/1900483‑1900632                                                                             |                                                                          gAAGCAATCGCCGCCACATCAACGCCCTCTTCCAGCGCCACCAGCAGTTCTTTAGCACGTACAATACCGATGATTTAATcc                                                                       <  1:324556/81‑1 (MQ=255)          gccgccACATCAACGCCCTCTTCCAGCGCCACCAGCAGTTCTTTAGCACGTACAATACCGATGATTTAATCCAGTTCAcc                                                               <  1:324557/80‑1 (MQ=255)          gccgccACATCAACGCCCTCTTCCAGCGCCACCAGCAGTTCTTTAGCACGTACAATACCGATGATTTAATCCAGTTCACcg                                                              >  1:324589/1‑81 (MQ=255)                acaTCAACGCCCTCTTCCAGCGCCACCAGCAGTTCTTTAGCACGTACAATACCGATGATTTAATCCAGTTCACCGCGACa                                                         >  2:324587/1‑80 (MQ=255)                   tCAACGCCCTCTTCCAGCGCCACCAGCAGTTCTTTAGCACGTACAATACCGATGATTTAATCCAGTTCACCGCGACATAcc                                                     >  2:324600/1‑81 (MQ=255)                     aaCGCCCTCTTCCAGCGCCACCAGCAGTTCTTTAGCACGTACAATACCGATGATTTAATCCAGTTCACCGCGACATACCgg                                                   >  2:324609/1‑81 (MQ=255)                     aaCGCCCTCTTCCAGCGCCACCAGCAGTTCTTTAGCACGTACAATACCGATGATTTAATCCAGTTCACCGCGACATACCgg                                                   >  1:324595/1‑81 (MQ=255)                     aaCGCCCTCTTCCAGCGCCACCAGCAGTTCTTTAGCACGTACAATACCGATGATTTAATCCAGTTCACCGCGACATACCg                                                    <  2:324558/80‑1 (MQ=255)                      aCGCCCTCTTCCAGCGCCACCAGCAGTTCTTTAGCACGTACAATACCGATGATTTAATCCAGTTCACCGCGACATACCggg                                                  <  2:324559/81‑1 (MQ=255)                         cccTCTTCCAGCGCCACCAGCAGTTCTTTAGCACGTACAATACCGATGATTTAATCCAGTTCACCGCGACATACCGGGAAc                                               <  1:324560/81‑1 (MQ=255)                                ccAGCGCCACCAGCAGTTCTTTAGCACGTACAATACCGATGATTTAATCCAGTTCACCGCGACATACCGGGAACAGAc                                           <  2:324561/78‑1 (MQ=255)                                  aGCGCCACCAGCAGTTCTTTAGCACGTACAATACCGATGATTTAATCCAGTTCACCGCGACATACCGGGAACAGACTGTGc                                      >  2:324614/1‑81 (MQ=255)                                     gcCACCAGCAGTTCTTTAGCACGTACAATACCGATGATTTAATCCAGTTCACCGCGACATACCGGGAACAGACTGTGCGGt                                   >  1:324611/1‑81 (MQ=255)                                     gcCACCAGCAGTTCTTTAGCACGTACAATACCGATGATTTAATCCAGTTCACCGCGACATACCGGGAACAGACTGTGCGGt                                   <  1:324562/81‑1 (MQ=255)                                       caccaGCAGTTCTTTAGCACGTACAATACCGATGATTTAATCCAGTTCACCGCGACATACCGGGAACAGACTGTGCGGTGa                                 <  2:324563/81‑1 (MQ=255)                                          cagcagTTCTTTAGCACGTACAATACCGATGATTTAATCCAGTTCACCGCGACATACCGGGAACAGACTGTGCGGTGaa                                >  1:324592/1‑79 (MQ=255)                                           agcagTTCTTTAGCACGTACAATACCGATGATTTAATCCAGTTCACCGCGACATACCGGGAACAGACTGTGCGGTGaa                                >  1:324601/1‑78 (MQ=255)                                               gTTCTTTAGCACGTACAATACCGATGATTTAATCCAGTTCACCGCGACATACCGGGAACAGACTGTGCGGTGAagagag                           >  1:324616/1‑79 (MQ=255)                                                  cTTTAGCACGTACAATACCGATGATTTAATCCAGTTCACCGCGACATACCGGGAACAGACTGTGCGGTGAAGAGAGCAGt                       <  1:324564/80‑1 (MQ=255)                                                     tAGCACGTACAATACCGATGATTTAATCCAGTTCACCGCGACATACCGGGAACAGACTGTGCGGTGAAGAGAGCAGtt                      <  1:324565/78‑1 (MQ=255)                                                      aGCACGTACAATACCGATGATTTAATCCAGTTCACCGCGACATACCGGGAACAGACTGTGCGGTGAAGAGAGCAGTTGCt                   <  2:324566/80‑1 (MQ=255)                                                      aGCACGTACAATACCGATGATTTAATCCAGTTCACCGCGACATACCGGGAACAGACTGTGCGGTGAAGAGAGCAGTTGCTc                  >  2:324602/1‑81 (MQ=255)                                                        cACGTACAATACCGATGATTTAATCCAGTTCACCGCGACATACCGGGAACAGACTGTGCGGTGAAGAGAGCAGTTGCTcgc                >  1:324599/1‑81 (MQ=255)                                                          cGTACAATACCGATGATTTAATCCAGTTCACCGCGACATACCGGGAACAGACTGTGCGGTGAAGAGAGCAGTTGCTCGCgg              <  1:324567/81‑1 (MQ=255)                                                              cagtACCGATGATTTAATCCAGTTCACCGCGACATACCGGGAACAGACTGTGCGGTGAAGAGAGCAGTTGCTCGCGGAtt           <  2:324568/77‑1 (MQ=255)                                                                aTACCGATGATTTAATCCAGTTCACCGCGACATACCGGGAACAGACTGTGCGGTGAAGAGAGCAGTTGCTCGCGGATTTCa        >  1:324610/1‑81 (MQ=255)                                                                     gatgatTTAATCCAGTTCACCGCGACATACCGGGAACAGACTGTGCGGTGAAGAGAGCAGTTGCTCGCGGATTTCATCGAc   >  1:324615/1‑81 (MQ=255)                                                                      atgatTTAATCCAGTTCACCGCGACATACCGGGAACAGACTGTGCGGTGAAGAGAGCAGTTGCTCGCGGATTTCATCGAcc  <  2:324569/81‑1 (MQ=255)                                                                             |                                                                          GAAGCAATCGCCGCCACATCAACGCCCTCTTCCAGCGCCACCAGCAGTTCTTTAGCACGTACAATACCGATGATTTCATCCAGTTCACCGCGACATACCGGGAACAGACTGTGCGGTGAAGAGAGCAGTTGCTCGCGGATTTCATCGACC  >  NC_000913/1900483‑1900632

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑