Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,905,042	G→A	G81D (GGT→GAT)	yobD →	conserved inner membrane protein YobD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,905,042	0	G	A	100.0%	73.9 / NA	22	G81D (GGT→GAT)	yobD	conserved inner membrane protein YobD
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (7/15);  total (7/15)

CTTATCTATAACAACGTCACGAATCATGGTGCGTTAATAACGACATGGTTATTAAGCGCACTGGCTCTGATGGGTTTTTATATATTCTGGATCCGCGTTCCGAAGATCATCTTTAAACAAAAAGGTTTTTTCTTCGCCAATGTCTGGAT  >  NC_000913/1904969‑1905117                                                                          |                                                                            cTTATCTATAACAACGTCACGAATCATGGTGCGTTAATAACGACATGGTTATTAAGCGCACTGGCTCTGATGGATTTTta                                                                       <  2:325347/80‑1 (MQ=255)     tCTATAACAACGTCACGAATCATGGTGCGTTAATAACGACATGGTTATTAAGCGCACTGGCTCTGATGGATTTTTatatat                                                                  >  2:325369/1‑81 (MQ=255)         taACAACGTCACGAATCATGGTGCGTTAATAACGACATGGTTATTAAGCGCACTGGCTCTGATGGATTTTTATATATTCt                                                               <  2:325348/80‑1 (MQ=255)           acaacGTCACGAATCATGGTGCGTTAATAACGACATGGTTATTAAGCGCACTGGCTCTGATGGATTTTTATATATTCTgg                                                             <  1:325349/80‑1 (MQ=255)           acaacGTCACGAATCATGGTGCGTTAATAACGACATGGTTATTAAGCGCACTGGCTCTGATGGATTTTTATATATTCTGGa                                                            <  1:325350/81‑1 (MQ=255)                 tCACGAATCATGGTGCGTTAATAACGACATGGTTATTAAGCGCACTGGCTCTGATGGATTTTTATATATTCTGGATCcgcg                                                      <  2:325351/81‑1 (MQ=255)                   aCGAATCATGGTGCGTTAATAACGACATGGTTATTAAGCGCACTGGCTCTGATGGATTTTTATATATTCTGGATCCGCGtt                                                    <  1:325352/81‑1 (MQ=255)                       aTCATGGTGCGTTAATAACGACATGGTTATTGAGCGCACTGGCTCTGATGGATTTTTATATATTCTGGATCCGCGTTCCGa                                                <  2:325353/81‑1 (MQ=255)                          aTGGTGCGTTAATAACGACATGGTTATTAAGCGCACTGGCTCTGATGGATTTTTATATATTCTGGATCCGCGTTCCGAAg                                              <  1:325354/80‑1 (MQ=255)                             gTGCGTTAATAACGACATGGTTATTAAGCGCACTGGCTCTGATGGATTTTTATATATTCTGGATCCGCGTTCCGAAGatc                                           <  2:325355/80‑1 (MQ=255)                             gTGCGTTAATAACGACATGGTTATTAAGCGCACTGGCTCTGATGGATTTTTATATATTCTGGATCCGCGTTCCGAAGatc                                           <  2:325356/80‑1 (MQ=255)                             gTGCGTTAATAACGACATGGTTATTAAGCGCACTGGCTCTGATGGATTTTTATATATTCTGGATCCGCGTTCCGAAGatc                                           <  2:325357/80‑1 (MQ=255)                                cGTTAATAACGACATGGTTATTAAGCGCACTGGCTCTGATGGATTTTTATATATTCTGGATCCGCGTTCCGAAGatcatc                                        <  1:325358/80‑1 (MQ=255)                                     ataaCGACATGGTTATTAAGCGCACTGGCTCTGATGGATTTTTATATATTCTGGATCCGCGTTCCGAAGATCATCTTTaaa                                  >  2:325376/1‑81 (MQ=255)                                       aaCGACATGGTTATTAAGCGCACTGGCTCTGATGGATTTTTATATATTCTGGATCCGCGTTCCGAAGATCATCTTTAAAc                                 >  2:325377/1‑80 (MQ=255)                                                     taAGCGCACTGGCTCTGATGGATTTTTATATATTCTGGATCCGCGTTCCGAAGATCATCTTTAAACAAAAAGGTTTTttct                  >  1:325378/1‑81 (MQ=255)                                                       aGCGCACTGGCTCTGATGGATTTTTATATATTCTGGATCCGCGTTCCGAAGATCATCTTTAAACAAAAAGGTTTTttcttc                >  1:325375/1‑81 (MQ=255)                                                       aGCGCACTGGCTCTGATGGATTTTTATATATTCTGGATCCGCGTTCCGAAGATCATCTTTAAACAAAAAGGTTTTttcttc                <  1:325359/81‑1 (MQ=255)                                                         cgcACTGGCTCTGATGGATTTTTATATATTCTGGATCCGCGTTCCGAAGATCATCTTTAAACAAAAAGGTTTTTTCTTCGc              >  1:325380/1‑81 (MQ=255)                                                             cTGGCTCTGATGGATTTTTATATATTCTGGATCCGCGTTCCGAAGATCATCTTTAAACAAAAAGGTTTTTTCTTCGCCAAt          <  2:325360/81‑1 (MQ=255)                                                                gCTCTGATGGATTTTTATATATTCTGGATCCGCGTTCCGAAGATCATCTTTAAACAAAAAGGTTTTTTCTTCGCCAATg         <  2:325361/79‑1 (MQ=255)                                                                     gATGGATTTTTATATATTCTGGATCCGCGTTCCGAAGATCATCTTTAAACAAAAAGGTTTTTTCTTCGCCAATGTCTGGAt  >  2:325379/1‑81 (MQ=255)                                                                          |                                                                            CTTATCTATAACAACGTCACGAATCATGGTGCGTTAATAACGACATGGTTATTAAGCGCACTGGCTCTGATGGGTTTTTATATATTCTGGATCCGCGTTCCGAAGATCATCTTTAAACAAAAAGGTTTTTTCTTCGCCAATGTCTGGAT  >  NC_000913/1904969‑1905117

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑