Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,915,145	C→A	E130D (GAG→GAT)	proQ ←	RNA chaperone ProQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,915,145	0	C	A	100.0%	88.3 / NA	26	E130D (GAG→GAT)	proQ	RNA chaperone ProQ
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (9/17);  total (9/17)

GTTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCG  >  NC_000913/1915069‑1915218                                                                             |                                                                          gTTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTATCAc                                                                       <  2:326839/81‑1 (MQ=255)    tACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTATCACcag                                                                    >  2:326896/1‑81 (MQ=255)      cGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTATCACcagca                                                                  <  2:326840/81‑1 (MQ=255)           aGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTATCACCAGCAGTTGc                                                             >  1:326891/1‑81 (MQ=255)            gcgcCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTATCACCAGCAGTTGCg                                                            <  2:326841/81‑1 (MQ=255)              gcCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTATCACCAGCAGTTGCGGc                                                          <  1:326842/81‑1 (MQ=255)                         ggcgtggcgtAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTATCACCAGCAGTTGCGGCAGCTTCGCGtt                                               <  1:326843/81‑1 (MQ=255)                         ggcgtggcgtAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTATCACCAGCAGTTGCGGCAGCTTCGCGtt                                               >  2:326888/1‑81 (MQ=255)                          gcatggcgtAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTATCACCAGCAGTTGCGGCAGCTTCGCGttt                                              <  2:326844/78‑1 (MQ=255)                            gtggcgtAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTATCACCAGCAGTTGCGGCAGCTTCGCGttttt                                            >  1:326895/1‑81 (MQ=255)                             tggcgtAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTATCACCAGCAGTTGCGGCAGCTTCGCGtttttt                                           <  1:326845/81‑1 (MQ=255)                             tggcgtAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTATCACCAGCAGTTGCGGCAGCTTCGCGtttttt                                           >  1:326893/1‑81 (MQ=255)                                    gTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTATCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTg                                     <  2:326846/80‑1 (MQ=255)                                     tCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTATCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCt                                   <  2:326847/81‑1 (MQ=255)                                        gACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTATCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGtt                                <  2:326848/81‑1 (MQ=255)                                                  gCGTTCGCGGCGCGGTGCGTCTTCTTTATCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCtg                      >  1:326892/1‑81 (MQ=255)                                                       cgcggcgcggTGCGTCTTCTTTATCACCAGCAGTTGCGGTAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGcc                  <  1:390582/80‑1 (MQ=255)                                                       cgcggcgcggTGCGTCTTCTTTATCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGcc                  <  1:326849/80‑1 (MQ=255)                                                       cgcggcgcggTGCGTCTTCTTTATCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCt                 >  2:326907/1‑81 (MQ=255)                                                           gcgcggTGCGTCTTCTTTATCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAac             <  2:326850/81‑1 (MQ=255)                                                              cggTGCGTCTTCTTTATCACCAGCAGTTGCGACAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACAcg          <  2:326851/81‑1 (MQ=255)                                                                 tGCGTCTTCTTTATCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACgcgc       >  2:326906/1‑81 (MQ=255)                                                                   cGTCTTCTTTATCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCtt     >  2:326897/1‑81 (MQ=255)                                                                    gTCTTCTTTATCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCttt    <  2:326852/81‑1 (MQ=255)                                                                     tcttctTTATCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTc   <  1:326853/81‑1 (MQ=255)                                                                     tcttctTTATCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTc   <  1:326854/81‑1 (MQ=255)                                                                         ctttatCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCg  <  2:326855/78‑1 (MQ=255)                                                                             |                                                                          GTTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCG  >  NC_000913/1915069‑1915218

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑