Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,975,186	C→T	A47T (GCC→ACC)	cheA ←	chemotaxis protein CheA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,975,186	0	C	T	100.0%	92.4 / NA	27	A47T (GCC→ACC)	cheA	chemotaxis protein CheA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (11/16);  total (11/16)

GCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCTGCCCCTCCTTTGATCGAGTGGGCAGCCCGAAAGATGGCATTCAATTGTTCGGCATCTGGCGCTTCCGGCTGCAAAACCAGCAAATGCTGCTCCATGTCAG  >  NC_000913/1975108‑1975263                                                                               |                                                                              gCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCTGCCCCTCCTTTGATCGAGTGGGTAg                                                                             <  1:337236/81‑1 (MQ=255)    ggTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCTGCCCCTCCTTTGATCGAGTGGGTAGccc                                                                          <  1:337237/81‑1 (MQ=255)    ggTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCTGCCCCTCCTTTGATCGAGTGGGTAGccc                                                                          <  2:337238/81‑1 (MQ=255)        ttCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCTGCCCCTCCTTTGATCGAGTGGGTAGCCCGa                                                                        >  2:337276/1‑79 (MQ=255)          ccATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCTGCCCCTCCTTTGATCGAGTGGGTAGCCCGa                                                                        >  2:337285/1‑77 (MQ=255)           cATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCTGCCCCTCCTTTGATCGAGTGGGTAGCCCGAAAGAt                                                                   >  1:337279/1‑81 (MQ=255)               aGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCTGCCCCTCCTTTGATCGAGTGGGTAGCCCGAAAGATGGc                                                                <  1:337239/80‑1 (MQ=255)                  tGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCTGCCCCTCCTTTGATCGAGTGGGTAGCCCGAAAGATGGCATTc                                                            <  1:337240/81‑1 (MQ=255)                      tGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCTGCCCCTCCTTTGATCGAGTGGGTAGCCCGAAAGATGGCATTCAAtt                                                        >  2:337275/1‑81 (MQ=255)                      tGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCTGCCCCTCCTTTGATCGAGTGGGTAGCCCGAAAGATGGCATTCAAtt                                                        <  2:337241/81‑1 (MQ=255)                               gCAAAACGCTGAAGCCAAAAGTTCCTGCCCCTCCTTTGATCGAGTGGGTAGCCCGAAAGATGGCATTCAATTGTTCGGCAt                                               >  2:337272/1‑81 (MQ=255)                                    aCGCTGAAGCCAAAAGTTCCTGCCCCTCCTTTGATCGAGTGGGTAGCCCGAAAGATGGCATTCAATTGTTCGGCATCTGgc                                          <  2:337242/81‑1 (MQ=255)                                       cTGAAGCCAAAAGTTCCTGCCCCTCCTTTGATCGAGTGGGTAGCCCGAAAGATGGCATTCAATTGTTCGGCATCTGGCGCt                                       >  2:337291/1‑81 (MQ=255)                                       cTGAAGCCAAAAGTTCCTGCCCCTCCTTTGATCGAGTGGGTAGCCCGAAAGATGGCATTCAATTGTTCGGCATCTGGCGCt                                       <  1:337243/81‑1 (MQ=255)                                             ccAAAAGTTCCTGCCCCTCCTTTGATCGAGTGGGTAGCCCGAAAGATGGCATTCAATTATTCGGCATCTGGCGCTTCCGGc                                 <  1:337244/81‑1 (MQ=255)                                                 aaGTTCCTGCCCCTCCTTTGATCGAGTGGGTAGCCCGAAAGATGGCATTCAATTGTTCGGCATCTGGCGCTTCCGGCTGc                              >  2:337277/1‑80 (MQ=255)                                                      ccTGCCCCTCCTTTGATCGAGTGGGTAGCCCGAAAGATGGCATTCAATTGTTCGGCATCTGGCGCTTCCGGCTGCAAAAc                         <  2:337245/80‑1 (MQ=255)                                                         gCCCCTCCTTTGATCGAGTGGGTAGCCCGAAAGATGGCATTCAATTGTTCGGCATCTGGCGCTTCCGGCTGCAAAACCAg                      >  1:337281/1‑80 (MQ=255)                                                          ccccTCCTTTGATCGAGTGGGTAGCCCGAAAGATGGCATTCAATTGTTCGGCATCTGGCGCTTCCGGCTGCAAAACCAg                      <  2:337246/79‑1 (MQ=255)                                                              tcctTTGATCGAGTGGGTAGCCCGAAAGATGGCATTCAATTGTTCGGCATCTGGCGCTTCCGGCTGCAAAACCAGCAAAtg                >  2:337289/1‑81 (MQ=255)                                                                   tGATCGAGTGGGTAGCCCGAAAGATGGCATTCAATTTTTCGGCATCTGGCGCTTCCGGCTGCAAAACCAGCAAAtgctg             <  1:337247/79‑1 (MQ=255)                                                                    gATCGAGTGGGTAGCCCGAAAGATGGCATTCAATTGTTCGGCATCTGGCGCTTCCGGCTGCAAAACCAGCAAATgctgct           <  1:337248/80‑1 (MQ=255)                                                                    gATCGAGTGGGTAGCCCGAAAGATGGCATTCAATTGTTCGGCATCTGGCGCTTCCGGCTGCAAAACCAGCAAATGCTGCTc          >  1:337280/1‑81 (MQ=255)                                                                     aTCGAGTGGGTAGCCCGAAAGATGGCATTCAATTGTTCGGCATCTGGCGCTTCCGGCTGCAAAACCAGCAAATGCTGCTcc         <  2:337249/81‑1 (MQ=255)                                                                        gAGTGGGTAGCCCGAAAGATGGCATTCAATTGTTCGGCATCTGGCGCTTCCGGCTGCAAAACCAGCAAATGCTGCTCCAt       <  1:337251/80‑1 (MQ=255)                                                                        gAGTGGGTAGCCCGAAAGATGGCATTCAATTGTTCGGCATCTGGCGCTTCCGGCTGCAAAACCAGCAAATGCTGCTCCAt       <  1:337250/80‑1 (MQ=255)                                                                            gggTAGCCCGAAAGATGGCATTCAATTGTTCGGCATCTGGCGCTTCCGGCTGCAAAACCAGCAAATGCTGCTCCATGTCAg  >  1:337284/1‑81 (MQ=255)                                                                               |                                                                              GCAGGTTTTCCATCAGATGCGTGGTTTCCTGCAAAACGCTGAAGCCAAAAGTTCCTGCCCCTCCTTTGATCGAGTGGGCAGCCCGAAAGATGGCATTCAATTGTTCGGCATCTGGCGCTTCCGGCTGCAAAACCAGCAAATGCTGCTCCATGTCAG  >  NC_000913/1975108‑1975263

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑