Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	222,219	C→A	G143C (GGT→TGT)	metN ←	L‑methionine/D‑methionine ABC transporter ATP binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	222,219	0	C	A	100.0%	122.3 / NA	35	G143C (GGT→TGT)	metN	L‑methionine/D‑methionine ABC transporter ATP binding subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (13/22);  total (13/22)

GTGGCTTCATCACACAGCAATACTTTGGGATTGCTGGCTAACGCACGGGCAATTGCCACACGTTGTTTCTGCCCACCGGAAAGATTCGACGGGTAGCTATCATGCTTATCGCCAAGACCAACCAATGACAGCAATTCCGTCACGCGACGTTTGA  >  NC_000913/222143‑222296                                                                             |                                                                              gtggCTTCATCACACAGCAATACTTTGGGATTGCTGGCTAACGCACGGGCAATTGCCACACGTTGTTTCTGCCCACAgg                                                                             <  2:44475/79‑1 (MQ=255)  tggCTTCATCACACAGCAATACTTTGGGATTGCTGGCTAACGCACGGGCAATTGCCACACGTTGTTTCTGCCCACAGGaaa                                                                          <  2:44476/81‑1 (MQ=255)  tggCTTCATCACACAGCAATACTTTGGGATTGCTGGCTAACGCACGGGCAATTGCCACACGTTGTTTCTGCCCACAGGa                                                                            >  1:44535/1‑79 (MQ=255)    gCTTCATCACACAGCAATACTTTGGGATTGCTGGCTAACGCACGGGCAATTGCCACACGTTGTTTCTGCCCACAGGAAAg                                                                         <  1:44477/80‑1 (MQ=255)        catcaCACAGCAATACTTTGGGATTGCTGGCTAACGCACGGGCAATTGCCACACGTTGTTTCTGCCCACAGGAAAGAtt                                                                      <  1:44478/79‑1 (MQ=255)        catcaCACAGCAATACTTTGGGATTGCTGGCTAACGCACGGGCAATTGCCACACGTTGTTTCTGCCCACAGGAAAGATTc                                                                     <  1:44479/80‑1 (MQ=255)         atcaCACAGCAATACTTTGGGATTGCTGGCTAACGCACGGGCAATTGCCACACGTTGTTTCTGCCCACAGGAAAGATTCg                                                                    <  1:44480/80‑1 (MQ=255)          tcaCACAGCAATACTTTGGGATTGCTGGCTAACGCACGGGCAATTGCCACACGTTGTTTCTGCCCACAGGAAAGATTCGAc                                                                  <  1:44481/81‑1 (MQ=255)           cacacaGCAATACTTTGGGATTGCTGGCTAACGCACGGGCAATTGCCACACGTTGTTTCTGCCCACAGGAAAGATTCGACg                                                                 >  2:44538/1‑81 (MQ=255)                aGCAATACTTTGGGAGTGCTGGCTAACGCACGGGCAATTGCCACACGTTGTTTCTGCCCACAGGAAAGATTCGACGGGTAg                                                            <  2:44482/81‑1 (MQ=255)                 gCAATACTTTGGGATTGCTGGCTAACGCACGGGCAATTGCCACACGTTGTTTCTGCCCACAGGAAAGATTCGACGGGTAGc                                                           <  2:44483/81‑1 (MQ=255)                   aaTACTTTGGGATTGCTGGCTAACGCACGGGCAATTGCCACACGTTGTTTCTGCCCACAGGAAAGATTCGACGGGTAGCTa                                                         <  2:44484/81‑1 (MQ=255)                    aTACTTTGGGATTGCTGGCTAACGCACGGGCAATTGCCACACGTTGTTTCTGCCCACAGGAAAGATTCGACGGGTAGCTAt                                                        >  2:44521/1‑81 (MQ=255)                       cTTTGGGATTGCTGGCTAACGCACGGGCAATTGCCACACGTTGTTTCTGCCCACAGGAAAGATTCGACGGGTAGCTATCAt                                                     <  1:44485/81‑1 (MQ=255)                         ttGGGATTGCTGGCTAACGCACGGGCAATTGCCACACGTTGTTTCTGCCCACAGGAAAGATTCGACGGGTAGCTATCATGc                                                   >  1:44528/1‑81 (MQ=255)                          tGGGATTGCTGGCTAACGCACGGGCAATTGCCACACGTTGTTTCTGCCCACAGGAAAGATTCGACGGGTAGCTATCATGc                                                   <  1:44486/80‑1 (MQ=255)                            ggATTGCTGGCTAACGCACGGGCAATTGCCACACGTTGTTTCTGCCCACAGGAAAGATTCGACGGGTAGCTATCATGCTTa                                                >  2:44533/1‑81 (MQ=255)                            ggATTGCTGGCTAACGCACGGGCAATTGCCACACGTTGTTTCTGCCCACAGGAAAGATTCGACGGGTAGCTATCATGCTTa                                                >  1:44529/1‑81 (MQ=255)                              aTTGCTGGCTAACGCACGGGCAATTGCCACACGTTGTTTCTGCCCACAGGAAAGATTCGACGGGTAGCTATCATGCTTATc                                              <  1:44488/81‑1 (MQ=255)                              aTTGCTGGCTAACGCACGGGCAATTGCCACACGTTGTTTCTGCCCACAGGAAAGATTCGACGGGTAGCTATCATGCTTATc                                              <  2:44487/81‑1 (MQ=255)                                    ggCTAACGCACGGGCAATTGCCACACGTTGTTTCTGCCCACAGGAAAGATTCGACGGGTAGCTATCATGCTTATCGcc                                           >  1:44543/1‑78 (MQ=255)                                     gCTAACGCACGGGCAATTGCCACACGTTGTTTCTGCCCACAGGAAAGATTCGACGGGTAGCTATCATGCTTATCGCCaa                                         <  1:44489/79‑1 (MQ=255)                                         aCGCACGGGCAATTGCCACACGTTGTTTCTACCCACAGGAAAGATTCGACGGGTAGCTATCATGCTTATCGCCAAGacca                                    <  2:44490/80‑1 (MQ=255)                                               gggCAATTGCCACACGTTGTTTCTGCCCACAGGAAAGATTCGACGGGTAGCTATCATGCTTATCGCCAAGACCAACCAATg                             <  2:44492/81‑1 (MQ=255)                                               gggCAATTGCCACACGTTGTTTCTGCCCACAGGAAAGATTCGACGGGTAGCTATCATGCTTATCGCCAAGACCAACCAATg                             <  2:44491/81‑1 (MQ=255)                                               gggCAATTGCCACACGTTGTTTCCGCCCACAGGAAAGATTCGACGGGTAGCTATCATGCTTATCGCCAAGACCAACCAAt                              >  2:44536/1‑80 (MQ=255)                                                 gCAATTGCCACACGTTGTTTCTGCCCACAGGAAAGATTCGACGGGTAGCTATCATGCTTATCGCCAAGACCAACCAATGa                            >  1:44537/1‑80 (MQ=255)                                                   aaTTGCCACACGTTGTTTCTGCCCACAGGAAAGATTCGACGGGTAGCTATCATGCTTATCGCCAAGACCAACCAATGACAg                         >  2:44526/1‑81 (MQ=255)                                                      tGCCACACGTTGTTTCTGCCCACAGGAAAGATTCGACGGGTAGCTATCATGCTTATCGCCAAGACCAACCAATGACAGCaa                      >  2:44545/1‑81 (MQ=255)                                                         cacaCGTTGTTTCTGCCCACAGGAAAGATTCGACGGGTAGCTATCATGCTTATCGCCAAGACCAACCAATGACAGCAATTc                   <  2:44493/81‑1 (MQ=255)                                                          acacGTTGTTTCTGCCCACAGGAAAGATTCGACGGGTAGCTATCATGCTTATCGCCAAGACCAACCAATGACAGCAATTcc                  >  2:44515/1‑81 (MQ=255)                                                            acGTTGTTTCTGCCCACAGGAAAGATTCGACGGGTAGCTATCATGCTTATCGCCAAGACCAACCAATGACAGCAATTCCGt                <  1:44494/81‑1 (MQ=255)                                                             cGTTGTTTCTGCCCACAGGAAAGATTCGACGGGTAGCTATCATGCTTATCGCCAAGACCAACCAATGACAGCAATTCCGTc               <  2:44495/81‑1 (MQ=255)                                                                     cTGCCCACAGGAAAGATTCGACGGGTAGCTATCATGCTTATCGCCAAGACCAACCAATGACAGCAATTCCGTCACGCGAc        <  2:44496/80‑1 (MQ=255)                                                                     cTGCCCACAGGAAAGATTCGACGGGTAGCTATCATGCTTATCGCCAAGACCAACCAATGACAGCAATTCCGTCACGCGACg       >  2:44542/1‑81 (MQ=255)                                                                          cacaGGAAAGATTCGACGGGTAGCTATCATGCTTATCGCCAAGACCAACCAATGACAGCAATTCCGTCACGCGACGTTTGa  >  2:44534/1‑81 (MQ=255)                                                                             |                                                                              GTGGCTTCATCACACAGCAATACTTTGGGATTGCTGGCTAACGCACGGGCAATTGCCACACGTTGTTTCTGCCCACCGGAAAGATTCGACGGGTAGCTATCATGCTTATCGCCAAGACCAACCAATGACAGCAATTCCGTCACGCGACGTTTGA  >  NC_000913/222143‑222296

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑