BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,990,813	T→A	V378E (GTG→GAG)	tyrP →	tyrosine:H(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,990,813	0	T	A	96.3%	85.5 / NA	27	V378E (GTG→GAG)	tyrP	tyrosine:H(+) symporter
Reads supporting (aligned to +/- strand): ref base T (0/0); major base A (17/9); minor base G (0/1); total (17/10)
Fisher's exact test for biased strand distribution p-value = 3.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTTGACCTGGCAAAGCAGAAAGCACAATCCTCAGGCGGGTTACCGGGTCAAAGGTGGTCGTCCGGCGCTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTG  >  NC_000913/1990742‑1990886

gTTGACCTGGCAAAGCAGAAAGCACAATCCTCAGGCGGGTTACCGGGTCAAAGGTGGTCGTCCGGCGCTGGAGGTGGTGtt                                                                  >  2:339608/1‑81 (MQ=255)

   gACCTGGCAAAGCAGAAAGCACAATCCTCAGGCGGGTTACCGGGTCAAAGGTGGTCGTCCGGCGCTGGAGGTGGTGTTtc                                                                <  1:339585/80‑1 (MQ=255)

     ccTGGCAAAGCAGAAAGCACAATCCTCAGGCGGGTTACCGGGTCAAAGGTGGTCGTCCGGCGCTGGAGGTGGTGTTTctct                                                             >  2:339611/1‑81 (MQ=255)

        ggCAAAGCAGAAAGCACAATCCTCAGGCGGGTTACCGGGTCAAAGGTGGTCGTCCGGCGCTGGAGGTGGTGTTTCTCtgtg                                                          >  2:339617/1‑81 (MQ=255)

         gCAAAGCAGAAAGCACAATCCTCAGGCGGGTTACCGGGTCAAAGGTGGTCGTCCGGCGCTGGAGGTGGTGTTTCTCtgt                                                           <  2:339586/79‑1 (MQ=255)

            aaGCAGAAAGCACAATCCTCAGGCGGGTTACCGGGTCAAAGGTGGTCGTCCGGCGCTGGAGGTGGTGTTTCTCTGTGGTAt                                                      <  1:339587/81‑1 (MQ=255)

               cAGAAAGCACAATCCTCAGGCGGGTTACCGGGTCAAAGGTGGTCGTCCGGCGCTGGAGGTGGTGTTTCTCTGTGGTATTGc                                                   >  2:339628/1‑81 (MQ=255)

                aGAAAGCACAATCCTCAGGCGGGTTACCGGGTCAAAGGTGGTCGTCCGGCGCTGGAGGTGGTGTTTCTCTGTGGTATTGCt                                                  >  1:339630/1‑81 (MQ=255)

                        caATCCTCAGGCGGGTTACCGGGTCAAAGGTGGTCGTCCGGCGCTGGAGGTGGTGTTTCTCTGTGGTATTGCTGTGATTgg                                          <  1:339588/81‑1 (MQ=255)

                         aaTCCTCAGGCGGGTTACCGGGTCAAAGGTGGTCGTCCGGCGCTGGAGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGc                                         >  2:339619/1‑81 (MQ=255)

                         aaTCCTCAGGCGGGTTACCGGGTCAAAGGTGGTCGTCCGGCGCTGGAGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGc                                         >  1:339624/1‑81 (MQ=255)

                            ccTCAGGCGGGTTACCGGGTCAAAGGTGGTCGTCCTGCGCTGGAGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCTTg                                      >  2:339618/1‑81 (MQ=255)

                                 ggCGGGTTACCGGGTCAAAGGTGGTCGTCCGGCGCTGGAGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAAtt                                 >  1:339646/1‑81 (MQ=255)

                                  gCGGGTTACCGGGTCAAAGGTGGTCGTCCGGCGCTGGAGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAAttt                                <  1:339589/81‑1 (MQ=255)

                                         aCCGGGTCAAAGGTGGTCGTCCGGCGCTGGAGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTg                         >  1:339626/1‑81 (MQ=255)

                                          ccGGGTCAAAGGTGGTCGTCCGGCGCTGGAGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGc                        <  2:339591/81‑1 (MQ=255)

                                          ccGGGTCAAAGGTGGTCGTCCGGCGCTGGAGATGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGc                        <  2:339590/81‑1 (MQ=255)

                                                cAAAGGTGGTCGTCCGGCGCTGGAGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAgg                  >  2:339635/1‑81 (MQ=255)

                                                    ggtggtCGTCCGGCGCTGGAGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGgttg              >  1:339632/1‑81 (MQ=255)

                                                      tggtCGTCCGGCGCTGGGGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGgttgtt            <  1:339592/81‑1 (MQ=255)

                                                      tggtCGTCCGGCGCTGGAGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGgttgtt            <  2:339593/81‑1 (MQ=255)

                                                      tggtCGTCCGGCGCTGGAGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGgttgtt            <  2:339594/81‑1 (MQ=255)

                                                        gtcgtcCGGCGCTGGAGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTAc          >  2:339627/1‑81 (MQ=255)

                                                           gtcCGGCGCTGGAGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTAc          >  1:339629/1‑78 (MQ=255)

                                                             ccGGCGCTGGAGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAg       >  1:339636/1‑79 (MQ=255)

                                                             ccGGCGCTGGAGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAg       >  1:339623/1‑79 (MQ=255)

                                                                gcgcTGGAGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTg  >  1:339631/1‑81 (MQ=255)

GTTGACCTGGCAAAGCAGAAAGCACAATCCTCAGGCGGGTTACCGGGTCAAAGGTGGTCGTCCGGCGCTGGTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTG  >  NC_000913/1990742‑1990886

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`