Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,048,790	G→A	G1285S (GGT→AGT)	yeeJ →	inverse autotransporter adhesin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,048,790	0	G	A	100.0%	109.9 / NA	32	G1285S (GGT→AGT)	yeeJ	inverse autotransporter adhesin
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (19/13);  total (19/13)

ATCGCCGGTACCCCGCAGAACAGCTCCGGCAGCGTCATCACCGCCACAGTCGTTGATAATAATGGCTTTCCGGTGAAAGGTGTGACTGTGAACTTCACCAGCAACGCAGCGACAGCCGAAATGACGAACGGCGGTCAAGCCGTGACGAACGAA  >  NC_000913/2048712‑2048864                                                                               |                                                                           aTCGCCGGTACCCCGCAGAACAGCTCCGGCAGCGTCATCACCGCCACAGTCGTTGATAATAATGGCTTTCCGGTGAAAAgt                                                                          >  2:348760/1‑81 (MQ=255)  tCGCCGGTACCCCGCAGAACAGCTCCGGCAGCGTCATCACCGCCACAGTCGTTGATAATAATGGCTTTCCGGTGAAAAgtg                                                                         <  2:348740/81‑1 (MQ=255)    gCCGGTACCCCGCAGAACAGCTCCGGCAGCGTCATCACCGCCACAGTCGTTGATAATAATGGCTTTCCGGTGAAAAgtgt                                                                        >  1:348777/1‑80 (MQ=255)    gCCGGTACCCCGCAGAACAGCTCCGGCAGCGTCATCACCGCCACAGTCGTTGATAATAATGGCTTTCCGGTGAAAAgtgt                                                                        >  1:348773/1‑80 (MQ=255)       ggTACCCCGCAGAACAGCTCCGGCAGCGTCATCACCGCCACAGTCGTTGATAATAATGGCTTTCCGGTGAAAAGTGTGACt                                                                    <  1:348741/81‑1 (MQ=255)        gTACCCCGCAGAACAGCTCCGGCAGCGTCATCACCGCCACAGTCGTTGATAATAATGGCTTTCCGGTGAAAAGTGTGACtg                                                                   >  2:348804/1‑81 (MQ=255)            cccGCAGAACAGCTCCGGCAGCGTCATCACCGCCACAGTCGTTGATAATAATGGCTTTCCGGTGAAAAGTGTGACTGTGaa                                                               >  2:348792/1‑81 (MQ=255)              cGCAGAACAGCTCCGGCAGCGTCATCACCGCCACAGTCGTTGATAATAATGGCTTTCCGGTGAAAAGTGTGACTGTGAACt                                                             >  1:348794/1‑81 (MQ=255)                 aGAACAGCTCCGGCAGCGTCATCACCGCCACAGTCGTTGATAATAATGGCTTTCCGGTGAAAAGTGTGACTGTGAACTTCa                                                          >  1:348783/1‑81 (MQ=255)                 aGAACAGCTCCGGCAGCGTCATCACCGCCACAGTCGTTGATAATAATGGCTTTCCGGTGAAAAGTGTGACTGTGAACTTCa                                                          >  1:348788/1‑81 (MQ=255)                   aaCAGCTCCGGCAGCGTCATCACCGCCACAGTCGTTGATAATAATGGCTTTCCGGTGAAAAGTGTGACTGTGAACTTCAcc                                                        <  2:348742/81‑1 (MQ=255)                   aaCAGCTCCGGCAGCGTCATCACCGCCACAGTCGTTGATAATAATGGCTCTCCGGTGAAAAGTGTGACTGTGAACTTCAc                                                         >  2:348795/1‑80 (MQ=255)                      aGCTCCGGCAGCGTCATCACCGCCACAGTCGTTGATAATAATGGCTTTCCGGTGAAAAGTGTGACTGTGAACTTCACCAg                                                      >  1:348782/1‑80 (MQ=255)                      aGCTCCGGCAGCGTCATCACCGCCACAGTCGTTGATAATAATGGCTTTCCGGTGAAAAGTGTGACTGTGAACTTCACCAg                                                      >  1:348774/1‑80 (MQ=255)                            ggCAGCGTCATCACCGCCACAGTCGTTGATAATAATGGCTTTCCGGTGAAAAGTGTGACTGTGAACTTCACCAGCAACGCa                                               <  2:348743/81‑1 (MQ=255)                             gCAGCGTCATCACCGCCACAGTCGTTGATAATAATGGCTTTCCGGTGAAAAGTGTGACTGTGAACTTCACCAGCAACGCAg                                              <  2:348744/81‑1 (MQ=255)                              cAGCGTCATCACCGCCACAGTCGTTGATAATAATGGCTTTCCGGTGAAAAGTGTGACTGTGAACTTCACCAGCAACGCAGc                                             <  2:348745/81‑1 (MQ=255)                                gCGTCATCACCGCCACAGTCGTTGATAATAATGGCTTTCCGGTGAAAAGTGTGACTGTGAACTTCACCAGCAACGCAGCGa                                           <  2:348746/81‑1 (MQ=255)                                       caCCGCCACAGTCGTTGATAATAATGGCTTTCCGGTGAAAAGTGTGACTGTGAACTTCACCAGCAACGCAGCGACAGCCg                                     >  1:348789/1‑80 (MQ=255)                                              acaGTCGTTGATAATAATGGCTTTCCGGTGAAAAGTGTGACTGTGAACTTCACCAGCAACGCAGCGACAGCCGAAATGAc                              >  1:348786/1‑80 (MQ=255)                                                    gTTGATAATAATGGCTTTCCGGTGAAAAGTGTGACTGTGAACTTCACCAGCAACGAAGAGACAGCCGAAATAACGAAcggc                       <  2:348747/81‑1 (MQ=255)                                                     ttGATAATAATGGCTTTCCGGCGAAAAGTGTGACTGTGAACTTCACCAGCAACGCAGCGACAGCCGAAATGACGAAcggcg                      <  1:348748/81‑1 (MQ=255)                                                         taataaTGGCTTTCCGGTGAAAAGTGTGACTGTGAACTTCACCAGCAACGCAGCGACAGCCGAAATGACGAACGGCGGTCa                  <  2:348749/81‑1 (MQ=255)                                                            taatGGCTTTCCGGTGAAAAGTGTGACTGTGAACTTCACCAGCAACGCAGCGACAGCCGAAATGACGAACGGCGGTCAAGc               <  1:348750/81‑1 (MQ=255)                                                             aatGGCTTTCCGGTGAAAAGTGTGACTGTGAACTTCACCAGCAACGCAGTGACAGCCGAAATGACGAACGGCGGTCAAGcc              >  2:348805/1‑81 (MQ=255)                                                              atGGCTTTCCGGTGAAAAGTGTGACTGTGAACTTCACCAGCAACGCAGCGACAGCCGAAATGACGAACGGCGGTCAAGCCg             >  1:348755/1‑81 (MQ=255)                                                               tGGCTTTCCGGTGAAAAGTGTGACTGTGAACTTCACCAGCAACGCAGCGACAGCCGAAATGACGAACGGCTGTCAAGCCGt            >  2:348800/1‑81 (MQ=255)                                                                  cTTTCCGGTGAAAAGTGTGACTGTGAACTTCACCAGCAACGCAGCGACAGCCGAAATGACGAACGGCGGTCAAGCCGTGac         <  2:348751/81‑1 (MQ=255)                                                                     tCCGGTGAAAAGTGTGACTGTGAACTTCACCAGCAACGCAGCGACAGCCGAAATGACGAACGGCGGTCAAGCCGTGacgaa      >  1:348801/1‑81 (MQ=255)                                                                        ggTGAAAAGTGTGACTGTGAACTTCACCAGCAACGCAGCGACAGCCGAAATGACGAACGGCGGTCAAGCCGTGacgaacg    >  2:348802/1‑80 (MQ=255)                                                                        ggTGAAAAGTGTGACTGTGAACTTCACCAGCAACGCAGCGACAGCCGAAATGACGAACGGCGGTCAAGCCGTGacgaacg    >  2:348803/1‑80 (MQ=255)                                                                         gTGAAAAGTGTGACTGTGAACTTCACCAGCAACGCAGCGACAGCCGAAATGACGAACGGCGGTCAAGCCGTGAcgaacgaa  <  2:348752/81‑1 (MQ=255)                                                                               |                                                                           ATCGCCGGTACCCCGCAGAACAGCTCCGGCAGCGTCATCACCGCCACAGTCGTTGATAATAATGGCTTTCCGGTGAAAGGTGTGACTGTGAACTTCACCAGCAACGCAGCGACAGCCGAAATGACGAACGGCGGTCAAGCCGTGACGAACGAA  >  NC_000913/2048712‑2048864

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑