Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	233,614	C→T	M114I (ATG→ATA)	mltD ←	membrane‑bound lytic murein transglycosylase D

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	233,614	0	C	T	100.0%	73.7 / NA	22	M114I (ATG→ATA)	mltD	membrane‑bound lytic murein transglycosylase D
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (10/12);  total (10/12)

CTGCGGCATTGGCGCCAGACGTTGCGTGAGGATCAAAAGCGCTCTCCACTATGGGTAGTAGTACCAGTTCCATAGGCATGTTACGTTTTTTAACTTGCCCTGCTATCCAGTACATATACGGCTCTGCCCGTAAAGTTACATCGTGGA  >  NC_000913/233538‑233684                                                                             |                                                                       cTGCGGCATTGGCGCCAGACGTTGCGTGAGGATCAAAAGCGCTCTCCACTATGGGTAGTAGTACCAGTTCCATAGGTATGt                                                                    <  2:46660/81‑1 (MQ=255)               ccAGACGTTGCGTGAGGATCAAAAGCGCTCTCCACTATGGGTAGTAGTACCAGTTCCATAGGTATGTTACGTTTTTTAACt                                                      >  1:46702/1‑81 (MQ=255)                    cGTTGCGTGAGGATCAAAAGCGCTCTCCACTATGGGTAGTAGTACCAGTTCCATAGGTATGTTACGTTTTTTAACTTGccc                                                 <  1:46661/81‑1 (MQ=255)                        gCGTGAGGATCAAAAGCGCTCTCCACTATGGGTAGTAGTACCAGTTCCATAGGTATGTTACGTTTTTTAACTTGCCCTGc                                              <  2:46662/80‑1 (MQ=255)                          gTGAGGATCAAAAGCGCTCTCCACTATGGGTAGTAGTACCAGTTCCATAGGTATGTTACGTTTTTTAACTTGCCCTGCTAt                                           >  1:46696/1‑81 (MQ=255)                            gAGGATCAAAAGCGCTCTCCACTATGGGTAGTAGTACCAGTTCCATAGGTATGTTACGTTTTTTAACTTGCCCTGCTATc                                          >  1:46700/1‑80 (MQ=255)                                 tCAAAAGCGCTCTCCACTATGGGTAGTAGTACCAGTTCCATAGGTATGTTACGTTTTTTAACTTGCCCTGCTATCCAGTAc                                    <  1:46663/81‑1 (MQ=255)                                  cAAAAGCGCTCTCCACTATGGGTAGTAGTACCAGTTCCATAGGTATGTTACGTTTTTTAACTTGCCCTGCTATCCAGTACa                                   >  2:46691/1‑81 (MQ=255)                                   aaaaGCGCTCTCCACTATGGGTAGTAGTACCAGTTCCATAGGTATGTTACGTTTTTTAACTTGCCCTGCTATCCAGTACat                                  <  1:46664/81‑1 (MQ=255)                                            ctcCACTATGGGTAGTAGTACCAGTTCCATAGGTATGTTACGTTTTTTAACTTGCCCTGCTATCCAGTACATATACGGctc                         >  2:46693/1‑81 (MQ=255)                                            ctcCACTATGGGTAGTAGTACCAGTTCCATAGGTATGTTACGTTTTTTAACTTGCCCTGCTATCCAGTACATATACGGctc                         >  2:46701/1‑81 (MQ=255)                                             tcCACTATGGGTAGTAGTACCAGTTCCATAGGTATGTTACGTTTTTTAACTTGCCCTGCTATCCAGTACATATACGGct                          <  1:46665/79‑1 (MQ=255)                                               cACTATGGGTAGTAGTACCAGTTCCATAGGTATGTTACGTTTTTTAACTTGCCCTGCTATCCAGTACATATACGGctc                         <  1:46666/78‑1 (MQ=255)                                                 cTATGGGTAGTAGTACCAGTTCCATAGGTATGTTACGTTTTTTAACTTGCCCTGCTATCCAGTACATATACGGCTCTGccc                    <  1:46667/81‑1 (MQ=255)                                                 cTATGGGTAGTAGTACCAGTTCCATAGGTATGTTACGTTTTTTAACTTGCCCTGCTATCCAGTACATATACGGCTCTGccc                    <  2:46668/81‑1 (MQ=255)                                                   aTGGGTAGTAGTACCAGTTCCATAGGTATGTTACGTTTTTTAACTTGCCCTGCTATCCAGTACATATACGGCTCTGCCCg                   >  1:46688/1‑80 (MQ=255)                                                             gtaCCAGTTCCATAGGTATGTTACGTTTTTTAACTTGCCCTGCTATCCAGTACATATACGGCTCTGCCCGTAAAGTTACAt        <  1:46669/81‑1 (MQ=255)                                                             gtaCCAGTTCCATAGGTATGTTACGTTTTTTAACTTGCCCTGCTATCCAGTACATATACGGCTCTGCCCGTAAAGTTACAt        >  1:46707/1‑81 (MQ=255)                                                              taCCAGTTCCATAGGTATGTTACGTTTTTTAACTTGCCCTGCTATCCAGTACATATACGGCTCTGCCCGTAAAGTTACATc       <  1:46670/81‑1 (MQ=255)                                                                   gTTACATAGGTATGTTACGTTTTTTAACTTGACCTGCTATCCAGTACATATACGGCTCTGACCGTAAAGTTAAATCGTGga  <  2:867305/81‑1 (MQ=255)                                                                    ttCCATAGGTATGTTACGTTTTTTAACTTGCCCTGCTATCCAGTACATATACGGCTCTGCCCGTAAAGTTACATCGTg    >  2:46698/1‑78 (MQ=255)                                                                     tCCATAGGTATGTTACGTTTTTTAACTTGCCCTGCTATCCAGTACATATACGGCTCTGCCCGTAAAGTTACATCGTg    >  1:46695/1‑77 (MQ=255)                                                                             |                                                                       CTGCGGCATTGGCGCCAGACGTTGCGTGAGGATCAAAAGCGCTCTCCACTATGGGTAGTAGTACCAGTTCCATAGGCATGTTACGTTTTTTAACTTGCCCTGCTATCCAGTACATATACGGCTCTGCCCGTAAAGTTACATCGTGGA  >  NC_000913/233538‑233684

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑