Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,101,774	T→C	intergenic (‑30/+121)	wbbL ← / ← insH‑7	interrupted rhamnosyltransferase WbbL/IS5 transposase and trans‑activator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,101,774	0	T	C	100.0%	27.9 / NA	10	intergenic (‑30/+121)	wbbL/insH‑7	interrupted rhamnosyltransferase WbbL/IS5 transposase and trans‑activator
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (3/7);  total (3/7)

ATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTT  >  NC_000913/2101696‑2101822                                                                               |                                                 aTCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAAtcct                                                >  2:357381/1‑81 (MQ=255)    aacaacCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATccttct                                             <  2:357356/81‑1 (MQ=255)         ccGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATCCTTCTCGGCt                                        <  2:357357/81‑1 (MQ=255)          cGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATCCTTCTCGGCTg                                       <  1:357358/81‑1 (MQ=255)          cGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATCCTTCTCGGCTg                                       <  2:357359/81‑1 (MQ=255)                  aaTAGATACTTTCTTTAGGAATTTTTGTTTTGTTAAGGAAGGTGCGAATAAGCGGGGAAATCCTTCTCGGCTGACTCAGtc                               <  2:357360/81‑1 (MQ=25)                          ctttctttAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATCCTTCTCGGCTGACTCAGtcatttcat                        <  2:357361/80‑1 (MQ=255)                                    gAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATCCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGtt             >  2:357377/1‑81 (MQ=255)                                     aaTTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATCCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGttt            <  2:357362/81‑1 (MQ=255)                                     aaTTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATCCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGttc            >  2:357390/1‑80 (MQ=255)                                            gTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATCCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGa     >  1:357382/1‑81 (MQ=255)                                                tattaAGGAAGGTGCGAATAAGCGGGGAAATCCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGAttt  <  2:357363/80‑1 (MQ=21)                                                                               |                                                 ATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTT  >  NC_000913/2101696‑2101822

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑