Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,105,766	T→C	I98V (ATT→GTT)	wbbI ←	beta‑1,6‑galactofuranosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,105,766	0	T	C	85.7%	12.0 / ‑3.2	7	I98V (ATT→GTT)	wbbI	beta‑1,6‑galactofuranosyltransferase
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (5/1);  total (6/1)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.53e-01

ATTGTGACTTATGACCATATCACAGGTAGCAAGCCGCACAGAATCACTACCCCCTCCTCCTCTTAATTCATCAATATCATGAATCAGAGGTACTATTCTAAATTTTAGAAGGCGGTGAAAGAATGACAATATATGCCAAAATGGTT  >  NC_000913/2105692‑2105837                                                                           |                                                                        aTTGTGACTTATGACCATATCACAGGTAGCAAGCCGCACAGAATCACTACCCCCTCCTCCTCTTAATTCATCAACATCATg                                                                   <  2:357806/81‑1 (MQ=255)             gACCATATCACAGGTAGCAAGCCGCACAGAATCACTACCCCCTCCTCCTCTTAATTCATCAACATCATGAATCAGAGGTAc                                                       >  1:357814/1‑81 (MQ=255)                     cacaGGTAGCAAGCCGCACAGAATCACTACCCCCTCCTCCTCTTAATTCATCAACATCATGAATCAGAGGTACTATTCTa                                                >  2:357819/1‑80 (MQ=255)                                  ccGCACAGAATCACTACCCCCTCCTCCTCTTAATTCATCAACATCATGAATCAGAGGTACTATTCTAAATTTTAGAAGGCg                                  >  1:357820/1‑81 (MQ=255)                                    gCACAGAATCACTACCCCCTCCTCCTCTTAATTCATCAACATCATGAATCAGAGGTACTATTCTAAATTTTAGAAGGCGGt                                >  2:357813/1‑81 (MQ=255)                                           aTCACTACCCCCTCCTCCTCTTAATTCATCAACATCATGAATCAGAGGTACTATTCTAAATTTTAGAAGGCGGTGAAAGaa                         >  1:357815/1‑81 (MQ=255)                                                                  aTTCATCAATATCATGAATCAGAGGTACTATTCTAAATTTTAGAAGGCGGTGAAAGAATGACAATATATGCCAAAATGGtt  >  1:357821/1‑81 (MQ=255)                                                                           |                                                                        ATTGTGACTTATGACCATATCACAGGTAGCAAGCCGCACAGAATCACTACCCCCTCCTCCTCTTAATTCATCAATATCATGAATCAGAGGTACTATTCTAAATTTTAGAAGGCGGTGAAAGAATGACAATATATGCCAAAATGGTT  >  NC_000913/2105692‑2105837

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑