Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,108,868	T→A	G239G (GGA→GGT)	rfbX ←	polyisoprenol‑linked O‑antigen repeat unit flippase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,108,868	0	T	A	100.0%	18.8 / NA	7	G239G (GGA→GGT)	rfbX	polyisoprenol‑linked O‑antigen repeat unit flippase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (1/6);  total (1/6)

ATAGCCCTTGAAGCGCGTTTCTTATAGTATTGGCCGCATTAAAATTCCCTACGGACGTTGGTCCAGATATAAATCCCAGGATAATAACTATTCCCGTAGAATATAAACTAATAGCAGATGTGGAAATAAAAACATGAAAACCGTCTGCT  >  NC_000913/2108795‑2108943                                                                          |                                                                            aTAGCCCTTGAAGCGCGTTTCTTATAGTATTGGCCGCATTAAAATTCCCTACGGACGTTGGTCCAGATATAAAACCCAgg                                                                       <  2:358014/80‑1 (MQ=255) aTAGCCCTTGAAGCGCGTTTCTTATAGTATTGGCCGCATTAAAATTCCCTACGGACGTTGGTCCAGATATAAAACCCAGGa                                                                      <  1:358015/81‑1 (MQ=255)      ccTTGAAGCGCGTTTCTTATAGTATTGGCCGCATTAAAATTCCCTACGGACGTTGGTCCAGATATAAAACCCAGGataata                                                                 <  1:358016/81‑1 (MQ=255)                     cTTATAGTATTGGCCGCATTAAAATTCCCTACGGACGTTGGTCCAGATATAAAACCCAGGATAATAACTATTCCCGTAGaa                                                  >  1:358025/1‑81 (MQ=255)                                               cccTACGGACGTTGGTCCAGATATAAAACCCAGGATAATAACTATTCCCGTAGAATATAAACTAATAGCAGATGTGGaaa                         <  1:358017/80‑1 (MQ=255)                                                                  gATATAAAACCCAGGATAATAACTATTCCCGTAGAATATAAACTAATAGCAGATGTGGAAATAAAAACATGAAAACCGTCt     <  2:358018/81‑1 (MQ=255)                                                                      taAAACCCAGGATAATAACTATTCCCGTAGAATATAAACTAATAGCAGATGTGGAAATAAAAACATGAAAACCGTCTGCt  <  2:358019/80‑1 (MQ=255)                                                                          |                                                                            ATAGCCCTTGAAGCGCGTTTCTTATAGTATTGGCCGCATTAAAATTCCCTACGGACGTTGGTCCAGATATAAATCCCAGGATAATAACTATTCCCGTAGAATATAAACTAATAGCAGATGTGGAAATAAAAACATGAAAACCGTCTGCT  >  NC_000913/2108795‑2108943

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑