Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,153,777	T→C	noncoding (132/136 nt)	sibB →	small RNA SibB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,153,777	0	T	C	100.0%	122.3 / NA	35	noncoding (132/136 nt)	sibB	small RNA SibB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/14);  total (21/14)

GACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAACGCGTAG  >  NC_000913/2153702‑2153853                                                                            |                                                                             gACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCCTTccc                                                                         >  1:365906/1‑81 (MQ=255)   cTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCCTTCCCGt                                                                       >  1:365910/1‑81 (MQ=255)     aaGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCCTTCCCGttt                                                                     >  2:365909/1‑81 (MQ=255)              acGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCCTTCCCGTTTCAGCGTccc                                                            <  1:365874/81‑1 (MQ=255)                  tgatgaGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCCTTCCCGTTTCAGCGTCCCGCTg                                                        <  1:365875/81‑1 (MQ=255)                  tgatgaGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCCTTCCCGTTTCAGCGTCCCGCTg                                                        <  2:365876/81‑1 (MQ=255)                         tAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCCTTCCCGTTTCAGCGTCCCGCTGAAAtcgt                                                 >  1:365914/1‑81 (MQ=255)                          aGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCCTTCCCGTTTCAGCGTCCCGCTGAAAtcgtc                                                >  1:365913/1‑81 (MQ=255)                          aGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCCTTCCCGTTTCAGCGTCCCGCTGAAAtcgtc                                                >  2:365917/1‑81 (MQ=255)                                atcatGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCCTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCtt                                           <  2:365880/80‑1 (MQ=255)                                atcatGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCCTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCtt                                           <  1:365878/80‑1 (MQ=255)                                atcatGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCCTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCtt                                           <  2:365877/80‑1 (MQ=255)                                atcatGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCCTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCtt                                           <  2:365879/80‑1 (MQ=255)                                   atGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCCTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTAcct                                       >  2:365919/1‑81 (MQ=255)                                    tGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCCTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTAcctc                                      >  1:365915/1‑81 (MQ=255)                                     gACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCCTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTAcctcc                                     >  2:365920/1‑81 (MQ=255)                                        ccTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCCTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCttt                                  >  1:365923/1‑81 (MQ=255)                                         cTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCCTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTc                                 <  1:365881/81‑1 (MQ=255)                                           ttCCTTATTTATGGCCCCTTCCTCGGGAGGGGCCTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGc                               >  2:365907/1‑81 (MQ=255)                                              cTTATTTATGGCCCCTTCCTCGGGAGGGGCCTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCAt                            <  1:365882/81‑1 (MQ=255)                                                tatttatGGCCCCTTCCTCGGGAGGGGCCTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATg                           >  2:365922/1‑80 (MQ=255)                                                    tatGGCCCCTTCCTCGGGAGGGGCCTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAg                       >  2:365908/1‑80 (MQ=255)                                                     atGGCCCCTTCCTCGGGAGGGGCCTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAg                       >  1:365924/1‑79 (MQ=255)                                                         ccccTTCCTCGGGAGGGGCCTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCt                 <  1:365883/81‑1 (MQ=255)                                                          cccTTCCTCGGGAGGGGCCTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTa                <  1:365884/81‑1 (MQ=255)                                                           ccTTCCTCGGGAGGGGCCTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTAt               <  1:365885/81‑1 (MQ=255)                                                             ttCCTCGGGAGGGGCCTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCg             >  2:365932/1‑81 (MQ=255)                                                             ttCCTCGGGAGGGGCCTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCg             >  1:365925/1‑81 (MQ=255)                                                                cTCGGGAGGGGCCTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCt           >  1:365916/1‑80 (MQ=255)                                                                  cGGGAGGGGCCTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAAc        >  2:365931/1‑81 (MQ=255)                                                                  cGGGAGGGGCCTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAAc        >  1:365911/1‑81 (MQ=255)                                                                   gggAGGGGCCTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAAc        <  2:365886/80‑1 (MQ=255)                                                                    ggAGGGGCCTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAAcgc      >  2:365912/1‑81 (MQ=255)                                                                        gggCCTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAACGCGTAg  <  2:365887/81‑1 (MQ=255)                                                                         ggCCTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAAcgcg     >  2:365918/1‑77 (MQ=255)                                                                            |                                                                             GACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCGTCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTAACGCGTAG  >  NC_000913/2153702‑2153853

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑