Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,158,945	T→C	V187A (GTC→GCC)	mdtC →	multidrug efflux pump RND permease subunit MdtC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,158,945	0	T	C	100.0%	150.6 / NA	42	V187A (GTC→GCC)	mdtC	multidrug efflux pump RND permease subunit MdtC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (25/17);  total (25/17)

GGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGCGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAAC  >  NC_000913/2158871‑2159021                                                                           |                                                                             ggCTCCGACGATTTCGCAAATCGACGGTGTTGGTGCTGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGCCGGGCt                                                                        >  1:367101/1‑81 (MQ=255) ggCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGCCGGGCt                                                                        >  2:367107/1‑81 (MQ=255)   cTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGCCGGGCTGa                                                                      <  2:367059/81‑1 (MQ=255)    tCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGCCGGGCTGaa                                                                     >  2:367114/1‑81 (MQ=255)     ccGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGCCGGGCTGAAt                                                                    >  1:367096/1‑81 (MQ=255)       gacgaTTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGCCGGGCTGAATcc                                                                  >  2:367098/1‑81 (MQ=255)       gacgaTTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGCCGGGCTGAATcc                                                                  >  2:367109/1‑81 (MQ=255)        acgaTTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGCCGGGCTGAATCCg                                                                 <  2:367061/81‑1 (MQ=255)        acgaTTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGCCGGGCTGAATCCg                                                                 <  1:367060/81‑1 (MQ=255)          gaTTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGCCGGGCTGAATCCg                                                                 >  1:367111/1‑79 (MQ=255)          gaTTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGCCGGGCTGAATCCGCa                                                               <  2:367062/81‑1 (MQ=255)              tCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGCCGGGCTGAATCCGCAGgc                                                            <  1:367063/80‑1 (MQ=255)               cGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGCCGGGCTGAATCCGCAGgcgc                                                          >  2:367113/1‑81 (MQ=255)                 cAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGCCGGGCTGAATCCGCAGGCGCTg                                                        <  2:367064/81‑1 (MQ=255)                   aaTCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGCCGGGCTGAATCCGCAGGCGCTGtt                                                      >  2:367129/1‑81 (MQ=255)                    aTCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGCCGGGCTGAATCCGCAGGCGCTGtt                                                      >  1:367117/1‑80 (MQ=255)                       gACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGCCGGGCTGAATCCGCAGGCGCTGTTTAAt                                                  >  1:367099/1‑81 (MQ=255)                       gACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGCCGGGCTGAATCCGCAGGCGCTGTTTAAt                                                  >  2:367106/1‑81 (MQ=255)                        aCGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGCCGGGCTGAATCCGCAGGCGCTGTTTAAt                                                  >  2:367133/1‑80 (MQ=255)                         cGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGCCGGGCTGAATCCGCAGGCGCTGTTTAATCa                                                <  1:367065/81‑1 (MQ=255)                                   gatgtcgatgtcGGAGGCAGCTCACTGCCCGCCGTACGCGCCGGGCTGAATCCGCAGGCGCTGTTTAATCAGGGCGTGTCg                                      >  1:367122/1‑81 (MQ=255)                                    atgtcgatgtcgGAGGCAGCTCACTGCCCGCCGTACGCGCCGGGCTGAATCCGCAGGCACTGTTTAATCAGGGCGTGTCg                                      <  2:367066/80‑1 (MQ=255)                                            gtcgGAGGCAGCTCACTGCCCGCCGTACGCGCCGGGCTGAATCCGCAGGCGCTGTTTAATCAGGGCGTGTCGCTGgacgac                             >  1:367121/1‑81 (MQ=255)                                               ggAGGCAGCTCACTGCCCGCCGTACGCGCCGGGCTGAATCCGCAGGCGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTa                          >  1:367120/1‑81 (MQ=255)                                                gAGGCAGCTCACTGCCCGCCGTACGCGCCGGGCTGAATCCGCAGGCGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTAc                         <  1:367067/81‑1 (MQ=255)                                                 aGGCAGCTCACTGCCCGCCGTACGCGCCGGGCTGAATCCGCAGGCGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACg                        >  2:367130/1‑81 (MQ=255)                                                  ggCAGCTCACTGCCCGCCGTACGCGCCGGGCTGAATCCGCAGGCGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGc                       <  1:367068/81‑1 (MQ=255)                                                   gCAGCTCACTGCCCGCCGTACGCGCCGGGCTGAATCCGCAGGCGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCa                      <  1:367069/81‑1 (MQ=255)                                                     aGCTCACTGCCCGCCGTACGCGCCGGGCTGAATCCGCAGGCGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCAcc                    >  1:367118/1‑81 (MQ=255)                                                     aGCTCACTGCCCGCCGTACGCGCCGGGCTGAATCCGCAGGCGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCAcc                    <  1:367070/81‑1 (MQ=255)                                                        tCACTGCCCGCCGTACGCGCCGGGCTGAATCCGCAGGCGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCAccgcc                 >  2:367151/1‑81 (MQ=255)                                                        tCACTGCCCGCCGTACGCGCCGGGCTGAATCCGCAGGCGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCAccgcc                 >  1:367135/1‑81 (MQ=255)                                                           cTGCCCGCCGTACGCGCCGGGCTGAATCCGCAGGCGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTc              <  2:367071/81‑1 (MQ=255)                                                            tGCCCGCCGTACGCGCCGGGCTGAATCCGCAGGCGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGt               >  1:367132/1‑79 (MQ=255)                                                             gCCCGCCGTACGCGCCGGGCTGAATCCGCAGGCGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAg            >  2:367137/1‑81 (MQ=255)                                                              cccGCCGTACGCGCCGGGCTGAATCCGCAGGCGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGc           <  2:367072/81‑1 (MQ=255)                                                                cgccgTACGCGCCGGGCTGAATCCGCAGGCGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCaa         >  1:367126/1‑81 (MQ=255)                                                                   cgTACGCGCCGGGCTGAATCCGCAGGCGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGc      >  1:367124/1‑81 (MQ=255)                                                                    gTACGCGCCGGGCTGAATCCGCAGGCGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGcc     <  2:367073/81‑1 (MQ=255)                                                                    gTACGCGCCGGGCTGAATCCGCAGGCGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGc      >  1:367127/1‑80 (MQ=255)                                                                       cgcgCCGGGCTGAATCCGCAGGCGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAAc  <  2:367075/81‑1 (MQ=255)                                                                       cgcgCCGGGCTGAATCCGCAGGCGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAAc  <  1:367074/81‑1 (MQ=255)                                                                           |                                                                             GGCTCCGACGATTTCGCAAATCGACGGTGTTGGTGATGTCGATGTCGGAGGCAGCTCACTGCCCGCCGTACGCGTCGGGCTGAATCCGCAGGCGCTGTTTAATCAGGGCGTGTCGCTGGACGACGTACGCACCGCCGTCAGCAATGCCAAC  >  NC_000913/2158871‑2159021

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑