Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,182,770	C→A	A4A (GCG→GCT)	yegW ←	putative DNA‑binding transcriptional regulator YegW

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,182,770	0	C	A	100.0%	73.7 / NA	23	A4A (GCG→GCT)	yegW	putative DNA‑binding transcriptional regulator YegW
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (10/13);  total (10/13)

CGGCAAACTTAATATAAAGCGGCGTGTTATCCGCCGCTAAAATACGCTCGTTCAGTTGGGCAATCAGCTGGGTATGCGCTTGTTCCATTTGTCTTTTTCCTGCCGGGGAGTCTGGTGGGAGTATATCACTGCTTCTTATTTAAGGCGTTTCTT  >  NC_000913/2182694‑2182846                                                                             |                                                                             cGGCAAACTTAATATAAAGCGGCGTGTTATCCGCCGCTAAAATACGCTCGTTCAGTTGGGCAATCAGCTGGGTATGAGCtt                                                                          >  1:371273/1‑81 (MQ=255)  ggCAAACTTAATATAAAGCGGCGTGTTATCCGCCGCTAAAATACGCTCGTTCAGTTGGGCAATCAGCTGGGTATGAGCtt                                                                          <  1:371243/80‑1 (MQ=255)               taAAGCGGCGTGTTATCCGCCGCTAAAATACGCTCGTTCAGTTGGGCAATCAGCTGGGTATGAGCTTGTTCCATTTGTCtt                                                            >  2:371270/1‑81 (MQ=255)                   gcggcgTGTTATCCGCCGCTAAAATACGCTCGTTCAGTTGGGCAATCAGCTGGGTATGAGCTTGTTCCATTTGTCTTTTTc                                                        <  1:371244/81‑1 (MQ=255)                       cgTGTTATCCGCCGCTAAAATACGCTCGTTCAGTTGGGCAATCAGCTGGGTATGAGCTTGTTCCATTTGTCTTTTTCCTGc                                                    >  2:371267/1‑81 (MQ=255)                         tgtTATCCGCCGCTAAAATACGCTCGTTCAGTTGGGCAATCAGCTGGGTATGAGCTTGTTCCATTTGTCTTTTTCCTGCCg                                                  <  2:371245/81‑1 (MQ=255)                                     cTAAAATACGCTCGTTCAGTTGGGCAATCAGCTGGGTATGAGCTTGTTCCATTTGTCTTTTTCCTGCCGGGGAGTCtggtg                                      >  1:371268/1‑81 (MQ=255)                                        aaaTACGCTCGTTCAGTTGGGCAATCAGCTGGGTATGAGCTTGTTCCATTTGTCTTTTTCCTGCCGGGGAGTCtggtgg                                     <  2:371246/79‑1 (MQ=255)                                                  gTTCAGTTGGGCAATCAGCTGGGTATGAGCTTGTTCCATTTGTCTTTTTCCGGCCGGGGAGTCTGGTGGGAGTATATCAcg                         <  2:371247/81‑2 (MQ=255)                                                   ttCAGTTGGGCAATCAGCTGGGTATGAGCTTGTTCCATTTGTCTTTTTCCTGCCGGGGAGTCTGGTGGGAGTATATCACTg                        >  2:668842/1‑81 (MQ=255)                                                     cAGTTGGGCAATCAGCTGGGTATGAGCTTGTTCCATTTGTCTTTTTCCTGCCGGGGAGTCTGGTGGGAGTATATCACTGct                      >  1:371272/1‑81 (MQ=255)                                                      aGTTGGGCAATCAGCTGGGTATGAGCTTGTTCCATTTGTCTTTTTCCTGCCGGGGAGTCTGGTGGGAGTATATCACTGctt                     >  1:371271/1‑81 (MQ=255)                                                       gTTGGGCAATCAGCTGGGTATGAGCTTGTTCCATTTGTCTTTTTCCTGCCGGGGAGTCTGGTGGGAGTATATCACTGcttc                    <  2:371248/81‑1 (MQ=255)                                                          gggCAATCAGCTGGGTATGAGCTTGTTCCATTTGTCTTTTTCCTGCCGGGGAGTCTGGTGGGAGTATATCACTGcttctt                  <  2:371250/80‑1 (MQ=255)                                                          gggCAATCAGCTGGGTATGAGCTTGTTCCATTTGTCTTTTTCCTGCCGGGGAGTCTGGTGGGAGTATATCACTGcttctt                  <  1:371249/80‑1 (MQ=255)                                                            gCAATCAGCTGGGTATGAGCTTGTTCCATTTGTCTTTTTCCTGCCGGGGAGTCTGGTGGGAGTATATCACTGCTTCTTAtt               <  2:371251/81‑1 (MQ=255)                                                             cAATCAGCTGGGTATGAGCTTGTTCCATTTGTCTTTTTCCTGCCGGGGAGTCTGGTGGGAGTATATCACTGCTTCTTAttt              <  2:371252/81‑1 (MQ=255)                                                                tCAGCTGGGTATGAGCTTGTTCCATTTGTCTTTTTCCTGCCGGGGAGTCTGGTGGGAGTATATCACTGCTTCTTATTTAAg           <  2:371253/81‑1 (MQ=255)                                                                   gCTGGGTATGAGCTTGTTCCATTTGTCTTTTTCCTGCCGGGGAGTCTGGTGGGAGTATATCACTGCTTCTTATTTAAGGCg        <  1:371254/81‑1 (MQ=255)                                                                    cTGGGTATGAGCTTGTTCCATTTGTCTTTTTCCTGCCGGGGAGTCTGGTGGGAGTATATCACTGCTTCTTATTTAAGGCGt       >  1:371278/1‑81 (MQ=255)                                                                       ggTATGAGCTTGTTCCATTTGTCTTTTTCCTGCCGGGGAGTCTGGTGGGAGTATATCACTGCTTCTTATTTAAGGCGttt     <  2:371255/80‑1 (MQ=255)                                                                       ggTATGAGCTTGTTCCATTTGTCTTTTTCCTGCCGGGGAGTCTGGTGGGAGTATATCACTGCTTCTTATTTAAGGCGTTTc    >  1:371280/1‑81 (MQ=255)                                                                          aTGAGCTTGTTCCATTTGTCTTTTTCCTGCCGGGGAGTCTGGTGGGAGTATATCACTGCTTCTTATTTAAGGCGTTTCtt  >  1:371274/1‑80 (MQ=255)                                                                             |                                                                             CGGCAAACTTAATATAAAGCGGCGTGTTATCCGCCGCTAAAATACGCTCGTTCAGTTGGGCAATCAGCTGGGTATGCGCTTGTTCCATTTGTCTTTTTCCTGCCGGGGAGTCTGGTGGGAGTATATCACTGCTTCTTATTTAAGGCGTTTCTT  >  NC_000913/2182694‑2182846

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑