Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,222,467	G→A	D95N (GAT→AAT)	dld →	D‑lactate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,222,467	0	G	A	100.0%	136.6 / NA	39	D95N (GAT→AAT)	dld	D‑lactate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (27/12);  total (27/12)

ACAAAATTATTCTGATGCAGGCCGCCAATACAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCGATGTCGTTATCATCAGCACCCTGCGTCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAGGTGCTGGCCTAT  >  NC_000913/2222390‑2222541                                                                              |                                                                           aCAAAATTATTCTGATGCAGGCCGCCAATACAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCAATg                                                                         >  1:377927/1‑81 (MQ=255)  cAAAATTATTCTGATGCAGGCCGCCAATACAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCAATGt                                                                        <  1:377891/81‑1 (MQ=255)  cAAAATTATTCTGATGCAGGCCGCCAATACAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCAATGt                                                                        <  2:377890/81‑1 (MQ=255)   aaaaTTATTCTGATGCAGGCCGCCAATACAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCAATGTc                                                                       <  2:377892/81‑1 (MQ=255)      attattCTGATGCAGGCCTCCAATACAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCAATGTCGt                                                                     >  1:377921/1‑80 (MQ=255)        tattCTGATGCAGGCCGCCAATACAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCAATGTCGTTat                                                                  >  2:377940/1‑81 (MQ=255)          ttCTGATGCAGGCCGCCAATACAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCAATGTCGTTatc                                                                 <  1:377893/80‑1 (MQ=255)             tGATGCAGGCCGCCAATACAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCAATGTCGTTAtcatca                                                             >  1:377924/1‑81 (MQ=255)                    ggCCGCCAATACAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCAATGTCGTTATCATCAGCACCCt                                                      >  2:377929/1‑81 (MQ=255)                    ggCCGCCAATACAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCAATGTCGTTATCATCAGCACCCt                                                      <  2:377894/81‑1 (MQ=255)                      ccgccAATACAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCAATGTCGTTATCATCAGCACCCTGc                                                    >  1:377926/1‑81 (MQ=255)                       cgccAATACAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCAATGTCGTTATCATCAGCACCCTGCg                                                   >  2:377930/1‑81 (MQ=255)                           aaTACAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCAATGTCGTTATCATCAGCACCCTGCGtctc                                               >  2:377939/1‑81 (MQ=255)                              aCAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCAATGTCGTTATCATCAGCACCCTGCGTCTCGa                                             >  1:377935/1‑80 (MQ=255)                                aGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCAATGTCGTTATCATCAGCACCCTGCGTCTCGAc                                            >  2:377944/1‑79 (MQ=255)                                 ggCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCAATGTCGTTATCATCAGCACCCTGCGTCTCGACAAg                                         >  1:377957/1‑81 (MQ=255)                                  gCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCAATGTCGTTATCATCAGCATCCTGCGTCTCGACAAGc                                        >  1:377925/1‑81 (MQ=255)                                    cTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCAATGTCGTTATCATCAGCACCCTGCGTCTCGACAAGCTg                                      <  2:377895/81‑1 (MQ=255)                                      gACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCAATGTCGTTATCATCAGCACCCTGCGTCTCGACAAGCTGCa                                    >  2:377941/1‑81 (MQ=255)                                      gACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCAATGTCGTTATCATCAGCACCCTGCGTCTCGACAAGCTGCa                                    >  2:377931/1‑81 (MQ=255)                                      gACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCAATGTCGTTATCATCAGCACCCTGCGTCTCGACAAGCTGCa                                    >  1:377954/1‑81 (MQ=255)                                          gAAGGATCGACGCCAAACGGTAACGATTATGATCGCAATGTCGTTATCATCAGCACCCTGCGTCTCGACAAGCTGCACGtt                                >  1:377959/1‑81 (MQ=255)                                          gAAGGATCGACGCCAAACGGTAACGATTATGATCGCAATGTCGTTATCATCAGCACCCTGCGTCTCGACAAGCTGCACGtt                                >  2:377937/1‑81 (MQ=255)                                             ggATCGACGCCAAACGGTAACCATTATTATCGCAATGTCGTTATCATCAGCACCCTGCGTCTCGACAAGCTGCACGTTCtt                             >  2:377928/1‑81 (MQ=255)                                              gATCGACGCCAAACGGTAACGATTATGATCGCAATGTCGTTATCATCAGCACCCTGCGTCTCGACAAGCTGCACGTTCTTg                            <  2:377896/81‑1 (MQ=255)                                                tCGACGCCAAACGGTAACGATTATGATCGCAATGTCGTTATCATCAGCACCCTGCGTCTCGACAAGCTGCACGTTCTTgg                           >  1:377976/1‑80 (MQ=255)                                                tCGACGCCAAACGGTAACGATTATGATCGCAATGTCGTTATCATCAGCACCCTGCGTCTCGACAAGCTGCACGTTCTTgg                           >  1:377961/1‑80 (MQ=255)                                                tCGACGCCAAACGGTAACGATTATGATCGCAATGTCGTTATCATCAGCACCCTGCGTCTCGACAAGCTGCACGTTCTTGGc                          <  1:377897/81‑1 (MQ=255)                                                        aaaCGGTAACGATTATGATCGCAATGTCGTTATCATCAGCACCCTGCGTCTCGACAAGCTGCACGTTCTTGGCAAGGGCg                   >  1:377950/1‑80 (MQ=255)                                                         aaCGGTAACGATTATGATCGCAATGTCGTTATCATCAGCACCCTGCGTCTCGACAAGCTGCACGTTCTTGGCAAGGGCGaa                 >  2:377933/1‑81 (MQ=255)                                                            ggTAACGATTATGATCGCAATGTCGTTATCATCAGCACCCTGCGTCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAg              >  2:377952/1‑81 (MQ=255)                                                            ggTAACGATTATGATCGCAATGTCGTTATCATCAGCACCCTGCGTCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAg              >  2:377934/1‑81 (MQ=255)                                                              tAACGATTATGATCGCAATGTCGTTATCATCAGCACCCTGCGTCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAGGt            >  2:377938/1‑81 (MQ=255)                                                                 cGATTATGATCGCAATGTCGTTATCATCAGCACCCTGCGTCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAGGTGCt         <  1:377898/81‑1 (MQ=255)                                                                 cGATTATGATCGCAATGTCGTTATCATCAGCACCCTGCGTCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAGGTGCt         >  1:377949/1‑81 (MQ=255)                                                                   aTTATGATCGCAATGTCGTTATCATCAGCACCCTGCGTCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAGGTGCTgg       <  1:377899/81‑1 (MQ=255)                                                                    ttATGATCGCAATGTCGTTATCATCAGCACCCTGCGTCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAGGTGCTGGc      <  2:377900/81‑1 (MQ=255)                                                                      aTGATCGCAATGTCGTTATCATCAGCACCCTGCGTCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAGGTGCTGGcc     >  2:377948/1‑80 (MQ=255)                                                                         aTCGCAATGTCGTTATCATCAGCACCCTGCGTCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAGGTGCTGGCCTAt  <  2:377901/80‑1 (MQ=255)                                                                              |                                                                           ACAAAATTATTCTGATGCAGGCCGCCAATACAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCGATGTCGTTATCATCAGCACCCTGCGTCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAGGTGCTGGCCTAT  >  NC_000913/2222390‑2222541

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑