| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,234,328 | G→A | G99D (GGC→GAC) | preT → | NAD‑dependent dihydropyrimidine dehydrogenase subunit PreT |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,234,328 | 0 | G | A | 100.0% | 67.7 / NA | 20 | G99D (GGC→GAC) | preT | NAD‑dependent dihydropyrimidine dehydrogenase subunit PreT |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (11/9); total (11/9) | |||||||||||
AGAGTGTGCCCGACGGAGAAATTATGCCAAAGCGGTTGTACCCGTGCCGGTGTTGATGCTCCCATTGATATCGGCCGCTTACAGCGTTTTGTTACTGATTTTGAACAACAAACCGGAATGGAAATTTATCAGCCCGGTACTAAAACGCTCG > NC_000913/2234255‑2234405 | agagTGTGCCCGACGGAGAGATTATGCCAAAGCGGTTGTACCCGTGCCGGTGTTGATGCTCCCATTGATATCGACCGCTTa > 2:379966/1‑81 (MQ=255) tgtgCCCGACGGAGAAATTATGCCAAAGCGGTTGTACCCGTGCCGGTGTTGATGCTCCCATTGATATCGACCGCTTACAGc < 2:379946/81‑1 (MQ=255) tgCCCGACGGAGAAATTATGCCAAAGCGGTTGTACCCGTGCCGGTGTTGATGCTCCCATTGATATCGACCGCTTACAGCg < 1:379947/80‑1 (MQ=255) cccGACGGAGAAATTATGCCAAAGCGGTTGTACCCGTGCCGGTGTTGATGCTCCCATTGATATCGACCGCTTACAGCGttt < 2:379948/81‑1 (MQ=255) aCGGAGAAATTATGCCAAAGCGGTTGTACCCGTGCCGGTGTTGATGCTCCCATTGATATCGACCGCTTACAGCGTTTTGt > 1:379981/1‑80 (MQ=255) gagaAATTATGCCAAAGCGGTTGTACCCGTGCCGGTGTTGATGCTCCCATTGATATCGACCGCTTACAGCGTTTTGTTACt > 2:379975/1‑81 (MQ=255) agaAATTATGCCAAAGCGGTTGTACCCGTGCCGGTGTTGATGCTCCCATTGATATCGACCGCTTACAGCGTTTTGTTACt > 2:379969/1‑80 (MQ=255) ccAAAGCGGTTGTACCCGTGCCGGTGTTGATGCTCCCATTGATATCGACCGCTTACAGCGTTTTGTTACTGATTTTGaaca < 1:379949/81‑1 (MQ=255) cAAAGCGGTTGTACCCGTGCCGGTGTTGATGCTCCCATTGATATCGACCGCTTACAGCGTTTTGTTACTGATTTTGaacaa < 2:379950/81‑1 (MQ=255) gTTGTACCCGTGCCGGTGTTGATGCTCCCATTGATATCGACCGCTTACAGCGTTTTGTTACTGATTTTGAACAACAAACCg > 2:379977/1‑81 (MQ=255) tGTACCCGTGCCGGTGTTGATGCTCCCATTGATATCGACCGCTTACAGCGTTTTGTTACTGATTTTGAACAACAAACCgg > 1:379984/1‑80 (MQ=255) cGTGCCGGTGTTGATGCTCCCATTGATATCGACCGCTTACAGCGTTTTGTTACTGATTTTGAACAACAAACCGGAATGGa > 1:379973/1‑80 (MQ=255) gCCGGTGTTGATGCTCCCATTGATATCGACCGCTTACAGCGTTTTGTTACTGATTTTGAACAACAAACCGGAATGGAAAtt > 2:379979/1‑81 (MQ=255) ggTGTTGATGCTCCCATTGATATCGACCGCTTACAGCGTTTTGTTACTGATTTTGAACAACAAACCGGAATGGAAATTTAt < 2:379951/81‑1 (MQ=255) cTCCCATTGATATCGACCGCTTACAGCGTTTTGTTACTGATTTTGAACAACAAACCGGAATGGAAATTTATCAGCCCGGTa < 1:379952/81‑1 (MQ=255) aTTGATATCGACCGCTTACAGCGTTTTGTTACTGATTTTGAACAACAAACCGGAATGGAAATTTATCAGCCCGGTACTaa > 2:379976/1‑80 (MQ=255) tatCGACCGCTTACAGCGTTTTGTTACTGATTTTGAACAACAAACCGGAATGGAAATTTATCAGCCCGGTACTAAAACGCt > 1:379978/1‑81 (MQ=255) atCGACCGCTTACAGCGTTTTGTTACTGATTTTGAACAACAAACCGGAATGGAAATTTATCAGCCCGGTACTAAAACGCTc > 2:379986/1‑81 (MQ=255) tCGACCGCTTACAGCGTTTTGTTACTGATTTTGAACAACAAACCGGAATGGAAATTTATCAGCCCGGTACTAAAACGCTCg < 2:379954/81‑1 (MQ=255) tCGACCGCTTACAGCGTTTTGTTACTGATTTTGAACAACAAACCGGAATGGAAATTTATCAGCCCGGTACTAAAACGCTCg < 1:379953/81‑1 (MQ=255) | AGAGTGTGCCCGACGGAGAAATTATGCCAAAGCGGTTGTACCCGTGCCGGTGTTGATGCTCCCATTGATATCGGCCGCTTACAGCGTTTTGTTACTGATTTTGAACAACAAACCGGAATGGAAATTTATCAGCCCGGTACTAAAACGCTCG > NC_000913/2234255‑2234405 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |